cDNA Clone TAK1-S

• Plasmid: pFB TAP TAK1 45-303 TAB1 437-489
• Parent Plasmid: pFastbac 6His TAP 3C
• Expressed: 6His-TAP-TAK1 45-303 TAB1 437-489
• Genbank: NM_006116 and NM_003188.
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGA
TGTTGCTATTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTG
TAGAGCTTCGGCAGTTATCCCGTGTGAACCATCCTAATATTGTAAAGCTT
TATGGAGCCTGCTTGAATCCAGTGTGTCTTGTGATGGAATATGCTGAAGG
GGGCTCTTTATATAATGTGCTGCATGGTGCTGAACCATTGCCATATTATA
CTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGAGTGGCT
TATCTTCACAGCATGCAACCCAAAGCGCTAATTCACAGGGACCTGAAACC
ACCAAACTTACTGCTGGTTGCAGGGGGGACAGTTCTAAAAATTTGTGATT
TTGGTACAGCCTGTGACATTCAGACACACATGACCAATAACAAGGGGAGT
GCTGCTTGGATGGCACCTGAAGTTTTTGAAGGTAGTAATTACAGTGAAAA
ATGTGACGTCTTCAGCTGGGGTATTATTCTTTGGGAAGTGATAACGCGTC
GGAAACCCTTTGATGAGATTGGTGGCCCAGCTTTCCGAATCATGTGGGCT
GTTCATAATGGTACTCGACCACCACTGATAAAAAATTTACCTAAGCCCAT
TGAGAGCCTGATGACTCGTTGTTGGTCTAAAGATCCTTCCCAGCGCCCTT
CAATGGAGGAAATTGTGAAAATAATGACTCACTTGATGCGGTACTTTCCA
GGAGCAGATGAGCCATTACAGTATCCTTGTCAGGAATTCGGAGGAGGAGG
AGGACAAAGCCCGACCTTAACCCTGCAGTCCACCAACACGCACACGCAGA
GCAGCAGCTCCAGCTCTGACGGAGGCCTCTTCCGCTCCCGGCCCGCCCAC
TCGCTCCCGCCTGGCGAGGACGGTCGTGTTGAGCCCTATGTGGACTTTGC
TGAGTTTTACCGCTAATGGAGCGTGGACCATAAGGGCGAATTCCAGCACA
CTGGCGGCCGTTACTAGTGGATCC

Amino Acid Sequence

MHHHHHHENLYFQALATMDTSAHDEAVDNKFNKERQNAFYEILHLPNLNE
EQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNTFYEI
LHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAG
ASENLYFQGGRSMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEV
LFQGPLGSGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNH
PNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQ
CSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHM
TNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPA
FRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTH
LMRYFPGADEPLQYPCQEFGGGGGQSPTLTLQSTNTHTQSSSSSSDGGLF
RSRPAHSLPPGEDGRVEPYVDFAEFYR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8053
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His /Tap for baculovirus expression.; Estimated MW of expressed protein : 59030 Daltons