cDNA Clone PPM1K

• Plasmid: pGEX6P-1-PPM1K human
• Parent Plasmid: pGEX6P1
• Expressed: GST-PPM1K human
• Genbank: NP_689755
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCAACAGCTGCCTTAATTACTTTGGTCAGAAGTGGTGGGAA
CCAGGTGAGAAGGAGAGTGCTGCTAAGCTCCCGCCTGCTGCAGGACGACA
GGCGGGTGACACCCACGTGCCACAGCTCCACTTCAGAGCCTAGGTGTTCT
CGGTTTGACCCAGATGGTAGTGGGAGTCCAGCTACCTGGGACAATTTTGG
GATCTGGGATAACCGCATTGATGAGCCAATTCTGCTGCCACCCAGCATTA
AGTATGGCAAGCCAATTCCCAAAATCAGCTTGGAAAAGGTGGGGTGCGCC
TCACAGATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTCGCTCA
GCTGACAGATGAGGTCCTGTACTTTGCAGTGTATGATGGACACGGTGGAC
CTGCAGCAGCTGATTTCTGTCATACCCACATGGAGAAATGTATTATGGAT
TTGCTTCCTAAGGAGAAGAACTTGGAAACTCTGTTGACCTTGGCTTTTCT
AGAAATAGATAAAGCCTTTTCGAGTCATGCCCGCCTGTCTGCTGATGCAA
CTCTTCTGACCTCTGGGACTACTGCAACAGTAGCCCTATTGCGAGATGGT
ATTGAACTGGTTGTAGCCAGTGTTGGGGACAGCCGGGCTATTTTGTGTAG
AAAAGGAAAACCCATGAAGCTGACCATTGACCATACTCCAGAAAGAAAAG
ATGAAAAAGAAAGGATCAAGAAATGTGGTGGTTTTGTAGCTTGGAATAGT
TTGGGGCAGCCTCACGTAAATGGCAGGCTTGCAATGACAAGAAGTATTGG
AGATTTGGACCTTAAGACCAGTGGTGTCATAGCAGAACCTGAAACTAAGA
GGATTAAGTTACATCATGCTGATGACAGCTTCCTGGTCCTCACCACAGAT
GGAATTAACTTCATGGTGAATAGTCAAGAGATTTGTGACTTTGTCAATCA
GTGCCATGATCCCAACGAAGCAGCCCATGCGGTGACTGAACAGGCAATAC
AGTACGGTACTGAGGATAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCC
TGGGGAAAATATAAGAACTCTGAAATCAACTTCTCATTCAGCAGAAGCTT
TGCCTCCAGTGGACGATGGGCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTAALITLVRSGGNQVRR
RVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDN
RIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDE
VLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDK
AFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKP
MKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDL
KTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDP
NEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSG
RWA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8045
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 67792 Daltons