cDNA Clone RFC4

• Plasmid: pCMV-HA-1 RFC4
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-RFC4
• Genbank: NP_002907
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCC
GCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGA
ACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGT
GTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATC
TTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAA
CTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCT
GAACTTTTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGG
AATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAACTGTGT
CAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATT
CTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACG
TACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACT
ATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGC
TTCAAGCCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGC
CAAGAAGGAAAATGTCAAAATTAGTGATGAGGGAATAGCTTATCTTGTTA
AAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACATTTCTTCAAAGCGCT
ACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAGACAT
TGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTC
AGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGAT
GAGGGTCATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGT
AGAAAATAACTTATCTGATAAACAGAAGTCTATTATCACAGAAAAACTTG
CCGAAGTTGACAAATGCCTAGCGGATGGTGCTGATGAACATTTGCAACTC
ATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTTAAGC
GGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPV
PWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTI
LAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDG
KPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIE
PLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDL
RKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDK
LEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCL
ADGADEHLQLISLCATVMQQLSQNC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; Estimated MW of expressed protein : 41015 Daltons