cDNA Clone USP2 (rat)

• Plasmid: pEX USP2 rat 271-618 GST
• Parent Plasmid: pEX C-GST
• Expressed: USP2 rat 271-618 GST
• Genbank: BC085719.1
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCT
TGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGTCTGAGCAACACCC
GCGAGTTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGC
CATACCAGCAGTGCACACACAGCTCTCATGGAAGAGTTTGCAAAACTAAT
CCAGACCATATGGACGTCATCCCCCAATGATGTGGTGAGCCCATCTGAGT
TCAAGACCCAGATCCAGAGATATGCACCACGCTTCGTTGGCTATAATCAG
CAGGATGCTCAGGAATTCCTCCGTTTCCTTCTGGATGGCCTCCACAACGA
GGTGAACCGGGTGGCAGCAAGGCCTAAGCCCAGCCCTGAGAGCCTTGATC
ATCTCCCTGATGAAGAGAAAGGGCGACAGATGTGGAGGAAGTATCTAGAA
AGGGAAGACAGTCGGATTGGGGATCTCTTCGTTGGGCAGCTAAAGAGCTC
CCTGACGTGCACCGACTGCGGCTACTGCTCTACAGTGTTCGATCCCTTCT
GGGACCTCTCGTTGCCCATTGCAAAGAGAGGCTATCCTGAAGTGACGTTA
ATGGATTGTATGAGGCTCTTCACCAAAGAGGATGTGTTGGATGGGGATGA
GAAACCAACATGCTGCCGCTGCCGGGCCAGAAAACGATGTATAAAGAAGT
TCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGCTCCACCTGAAGCGGTTC
TCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCC
ACTAAGAGACCTGGACTTGAGAGAATTTGCTTCAGAAAACACCAACCATG
CTGTTTACAACCTGTATGCTGTGTCCAATCACTCCGGAACCACCATGGGA
GGTCACTATACAGCCTACTGCCGAAGTCCGGTTACGGGCGAATGGCACAC
TTTCAATGACTCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCA
GCGACGCCTATTTGCTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATG
GCGGCCGC

Amino Acid Sequence

MNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLGHT
SSAHTALMEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQD
AQEFLRFLLDGLHNEVNRVAARPKPSPESLDHLPDEEKGRQMWRKYLERE
DSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMD
CMRLFTKEDVLDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRFSE
SRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGH
YTAYCRSPVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRMAA
ALEVLFQGPLSRMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEG
DKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKE
RAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLC
HKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQ
IDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDL*

• Antibiotic: Amp
• DU Number: DU35693
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter GST for bacterial expression; ; Estimated MW of expressed protein : 67240 Daltons