cDNA Clone PLK1

• Plasmid: pEBG6P-PLK1 D194A
• Parent Plasmid: pEBG6P
• Expressed: GST PLK1 D194A
• Genbank: NM_005030
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACC
GGCCGACCCTGGGAAAGCCGGGGTCCCCGGAGTTGCAGCTCCCGGAGCTC
CGGCGGCGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCTAGTGGACCCA
CGCAGCCGGCGGCGCTATGTGCGGGGCCGCTTTTTGGGCAAGGGCGGCTT
TGCCAAGTGCTTCGAGATCTCGGACGCGGACACCAAGGAGGTGTTCGCGG
GCAAGATTGTGCCTAAGTCTCTGCTGCTCAAGCCGCACCAGAGGGAGAAG
ATGTCCATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCACGTCGT
AGGATTCCACGGCTTTTTCGAGGACAACGACTTCGTGTTCGTGGTGTTGG
AGCTCTGCCGCCGGAGGTCTCTCCTGGAGCTGCACAAGAGGAGGAAAGCC
CTGACTGAGCCTGAGGCCCGATACTACCTACGGCAAATTGTGCTTGGCTG
CCAGTACCTGCACCGAAACCGAGTTATTCATCGAGACCTCAAGCTGGGCA
ACCTTTTCCTGAATGAAGATCTGGAGGTGAAAATAGGGGCTTTTGGACTG
GCAACCAAAGTCGAATATGACGGGGAGAGGAAGAAGACCCTGTGTGGGAC
TCCTAATTACATAGCTCCCGAGGTGCTGAGCAAGAAAGGGCACAGTTTCG
AGGTGGATGTGTGGTCCATTGGGTGTATCATGTATACCTTGTTAGTGGGC
AAACCACCTTTTGAGACTTCTTGCCTAAAAGAGACCTACCTCCGGATCAA
GAAGAATGAATACAGTATTCCCAAGCACATCAACCCCGTGGCCGCCTCCC
TCATCCAGAAGATGCTTCAGACAGATCCCACTGCCCGCCCAACCATTAAC
GAGCTGCTTAATGACGAGTTCTTTACTTCTGGCTATATCCCTGCCCGTCT
CCCCATCACCTGCCTGACCATTCCACCAAGGTTTTCGATTGCTCCCAGCA
GCCTGGACCCCAGCAACCGGAAGCCCCTCACAGTCCTCAATAAAGGCTTG
GAGAACCCCCTGCCTGAGCGTCCCCGGGAAAAAGAAGAACCAGTGGTTCG
AGAGACAGGTGAGGTGGTCGACTGCCACCTCAGTGACATGCTGCAGCAGC
TGCACAGTGTCAATGCCTCCAAGCCCTCGGAGCGTGGGCTGGTCAGGCAA
GAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTG
GGTGGACTATTCGGACAAGTACGGCCTTGGGTATCAGCTCTGTGATAACA
GCGTGGGGGTGCTCTTCAATGACTCAACACGCCTCATCCTCTACAATGAT
GGTGACAGCCTGCAGTACATAGAGCGTGACGGCACTGAGTCCTACCTCAC
CGTGAGTTCCCATCCCAACTCCTTGATGAAGAAGATCACCCTCCTTAAAT
ATTTCCGCAATTACATGAGCGAGCACTTGCTGAAGGCAGGTGCCAACATC
ACGCCGCGCGAAGGTGATGAGCTCGCCCGGCTGCCCTACCTACGGACCTG
GTTCCGCACCCGCAGCGCCATCATCCTGCACCTCAGCAACGGCAGCGTGC
AGATCAACTTCTTCCAGGATCACACCAAGCTCATCTTGTGCCCACTGATG
GCAGCCGTGACCTACATCGACGAGAAGCGGGACTTCCGCACATACCGCCT
GAGTCTCCTGGAGGAGTACGGCTGCTGCAAGGAGCTGGCCAGCCGGCTCC
GCTACGCCCGCACTATGGTGGACAAGCTGCTGAGCTCACGCTCGGCCAGC
AACCGTCTCAAGGCCTCCTAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMSAA
VTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYV
RGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISI
HRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEAR
YYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGAFGLATKVEYD
GERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETS
CLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEF
FTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPER
PREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPA
CIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYI
ERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDE
LARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYID
EKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35606
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression