cDNA Clone TAPP1

• Synonyms: PLEKHA1
• Plasmid: pGEX4T1 TAPP1 R211L (from S.Dowler)
• Parent Plasmid: pGEX4T1
• Expressed: GST-Thrombin-myc-TAPP1 R211L
• Genbank: NM_021622.4 GI:307344639 with silent change c699c and coding change a958g (T320A - common variant) (as well as R211L change)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGAACAAAAACTCATCTCAGAAGAGGATCTGATGCCTTATGTGGATCGTCAGAATCGCATTTGTGGTTTTCTAGACATTGAAGAAAATGAAAACAGTGGGAAATTTCTTCGAAGGTACTTCATACTGGATACCAGAGAAGATAGTTTCGTGTGGTACATGGATAATCCACAGAACCTACCTTCTGGATCATCACGTGTTGGAGCCATTAAGCTTACCTACATTTCAAAGGTTAGCGATGCTACTAAGCTAAGGCCAAAGGCGGAGTTCTGTTTTGTTATGAATGCAGGAATGAGGAAGTACTTCCTACAAGCCAATGATCAGCAGGACCTAGTGGAATGGGTAAATGTGTTAAACAAAGCTATAAAAATTACAGTACCAAAGCAGTCAGACTCACAGCCTAATTCTGATAACCTAAGTCGCCATGGTGAATGTGGGAAAAAGCAAGTGTCTTACAGAACTGATATTGTTGGTGGCGTACCCATCATTACTCCCACTCAGAAAGAAGAAGTAAATGAATGTGGTGAAAGTATTGACAGAAATAATCTGAAACGGTCACAAAGCCATCTTCCTTACTTTACTCCTAAACCACCTCAAGATAGTGCGGTTATCAAAGCTGGATATTGTGTAAAACAAGGAGCAGTGATGAAAAACTGGAAGAGATTATATTTTCAATTGGATGAAAACACAATAGGCTACTTCAAATCTGAACTGGAAAAGGAACCTCTTCGCGTAATACCACTTAAAGAGGTTCATAAAGTCCAGGAATGTAAGCAAAGCGACATAATGATGAGGGACAACCTCTTTGAAATTGTAACAACGTCTCGAACTTTCTATGTGCAGGCTGATAGCCCTGAAGAGATGCACAGTTGGATTAAAGCAGTCTCTGGCGCCATTGTAGCACAGCGGGGTCCCGGCAGATCTGCGTCTTCTGAGCATCCCCCCGGTCCTTCAGAATCCAAACACGCTTTCCGTCCTACCAACGCAGCCGCCGCCACCTCACATTCCACAGCCTCTCGCAGCAACTCTTTGGTCTCAACCTTTACCATGGAGAAGCGAGGATTTTACGAGTCTCTTGCCAAGGTCAAGCCAGGGAACTTCAAGGTCCAGACTGTCTCTCCAAGAGAACCAGCTTCCAAAGTGACTGAACAAGCTCTGTTAAGACCTCAAAGTAAAAATGGCCCTCAGGAAAAAGATTGTGACCTAGTAGACTTGGACGATGCGAGCCTTCCGGTCAGTGACGTGTGAGGCAGAAGCGCACGGAGCCTGCCTGCCTCTGCCGTCCTCAGTTACCTTTCATGAGGCTTCTAGCCAAAGATGATAAAGGGGGAAATGGTTTTTAGTGCGTATATTATACTGCCTCTTAGGTGTACTCTTTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMEQKLISEEDLMPYVDRQNRIC
GFLDIEENENSGKFLRRYFILDTREDSFVWYMDNPQNLPSGSSRVGAIKL
TYISKVSDATKLRPKAEFCFVMNAGMRKYFLQANDQQDLVEWVNVLNKAI
KITVPKQSDSQPNSDNLSRHGECGKKQVSYRTDIVGGVPIITPTQKEEVN
ECGESIDRNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRL
YFQLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIV
TTSRTFYVQADSPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKH
AFRPTNAAAATSHSTASRSNSLVSTFTMEKRGFYESLAKVKPGNFKVQTV
SPREPASKVTEQALLRPQSKNGPQEKDCDLVDLDDASLPVSDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU13852
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee