cDNA Clone TAPP1

• Plasmid: pGEX-full TAPP1
• Parent Plasmid: pGEX4T1
• Expressed: GST-TAPP1
• Genbank: NM_021622
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGCCTTATGTGGATCGTCAGAATCGCATTTGTGGTTTTCTAGACATTGAAGAAAATGAAAACAGTGGGAAATTTCTTCGAAGGTACTTCATACTGGATACCAGAGAAGATAGTTTCGTGTGGTACATGGATAATCCACAGAACCTACCTTCTGGATCATCACGTGTTGGAGCCATTAAGCTTACCTACATTTCAAAGGTTAGCGATGCTACTAAGCTAAGGCCAAAGGCGGAGTTCTGTTTTGTTATGAATGCAGGAATGAGGAAGTACTTCCTACAAGCCAATGATCAGCAGGACCTAGTGGAATGGGTAAATGTGTTAAACAAAGCTATAAAAATTACAGTACCAAAGCAGTCAGACTCACAGCCTAATTCTGATAACCTAAGTCGCCATGGTGAATGTGGGAAAAAGCAAGTGTCTTACAGAACTGATATTGTTGGTGGCGTACCCATCATTACTCCCACTCAGAAAGAAGAAGTAAATGAATGTGGTGAAAGTATTGACAGAAATAATCTGAAACGGTCACAAAGCCATCTTCCTTACTTTACTCCTAAACCACCTCAAGATAGTGCGGTTATCAAAGCTGGATATTGTGTAAAACAAGGAGCAGTGATGAAAAACTGGAAGAGAAGATATTTTCAATTGGATGAAAACACAATAGGCTACTTCAAATCTGAACTGGAAAAGGAACCTCTTCGCGTAATACCACTTAAAGAGGTTCATAAAGTCCAGGAATGTAAGCAAAGCGACATAATGATGAGGGACAACCTCTTTGAAATTGTAACAACGTCTCGAACTTTCTATGTGCAGGCTGATAGCCCTGAAGAGATGCACAGTTGGATTAAAGCAGTCTCTGGCGCCATTGTAGCACAGCGGGGTCCCGGCAGATCTGCGTCTTCTGAGCATCCCCCCGGTCCTTCAGAATCCAAACACGCTTTCCGTCCTACCAACGCAGCCGCCGCCACCTCACATTCCACAGCCTCTCGCAGCAACTCTTTGGTCTCAACCTTTACCATGGAGAAGCGAGGATTTTACGAGTCTCTTGCCAAGGTCAAGCCAGGGAACTTCAAGGTCCAGACTGTCTCTCCAAGAGAACCAGCTTCCAAAGTGACTGAACAAGCTCTGTTAAGACCTCAAAGTAAAAATGGCCCTCAGGAAAAAGATTGTGACCTAGTAGACTTGGACGATGCGAGCCTTCCGGTCAGTGACGTGTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSATMDYKDDDDKPYVDRQNRICGFLDIEENENSGKFLRRYFILDTREDSFVWYMDNPQNLPSGSSRVGAIKLTYISKVSDATKLRPKAEFCFVMNAGMRKYFLQANDQQDLVEWVNVLNKAIKITVPKQSDSQPNSDNLSRHGECGKKQVSYRTDIVGGVPIITPTQKEEVNECGESIDRNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYFQLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQADSPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAAAATSHSTASRSNSLVSTFTMEKRGFYESLAKVKPGNFKVQTVSPREPASKVTEQALLRPQSKNGPQEKDCDLVDLDDASLPVSDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU10815
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dowler,S., Currie,R.A., Campbell,D.G., Deak,M., Kular,G.,Downes,C.P. and Alessi,D.R.; Identification of pleckstrin-homology-domain-containing proteins; with novel phosphoinositide-binding specificities; JOURNAL Biochem. J. 351 (PT 1), 19-31 (2000)