Plasmid
GFP-TAP SCYL3
Parent Plasmid
pEGFPC2
Expressed
GFP-TAP-SCYL3
Genbank
HS97P201
Tag
GFP
TAP
Species
Human
Sequence of Insert
View Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTCGATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACGCGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGATCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTCCAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGGATCAGAGAACAGTGCTTTAAAGAGCTATACACTGAGAGAACCACCATTTACCTTACCCTCTGGACTTGCTGTTTATCCCGCTGTACTGCAAGATGGCAAATTTGCTTCAGTTTTTGTGTATAAGAGAGAAAATGAAGACAAGGTTAATAAAGCTGCCAAGCATTTGAAGACACTTCGTCACCCTTGCTTGCTAAGATTTTTATCTTGTACTGTGGAAGCGGATGGCATTCATCTTGTCACTAAGCGAGTACAGCCCCTGGAAGTGGCTTTGGAAACATTGTCTTCTGCAGAGGTCTGTGCTGGGATCTATGACATATTGCTGGCTCTTATCTTCCTTCATGACAGAGGACACCTAACACACAATAATGTCTGTTTATCATCTGTGTTTGTGAGTGAAGATGGACACTGGAAGCTAGGAGGAATGGAAACTGTTTGTAAAGTTTCTCAGGCCACACCAGAGTTTCTGAGGAGTATTCAGTCAATAAGAGACCCAGCATCTATCCCTCCTGAAGAGATGTCTCCAGAATTCACAACTCTCCCAGAGTGTCATGGACATGCCCGGGATGCCTTTTCATTTGGAACATTGGTGGAAAGTTTGCTCACAATCTTAAATGAACAGGTTTCAGCGGATGTTCTCTCCAGCTTTCAACAGACCTTGCACTCAACTTTGCTGAATCCCATTCCAAAATGTCGGCCAGCGCTCTGCACCTTACTATCTCATGACTTCTTCAGAAATGATTTTCTGGAAGTTGTGAATTTCTTGAAAAGTTTAACATTGAAGAGTGAAGAGGAGAAAACGGAATTCTTTAAATTTCTGCTGGACAGAGTCAGCTGCTTGTCAGAGGAATTGATAGCTTCAAGGTTGGTGCCTCTTCTGCTTAATCAGTTGGTGTTTGCAGAGCCAGTGGCTGTTAAGAGTTTTCTTCCTTATCTGCTTGGCCCCAAAAAAGATCATGCGCAGGGAGAAACTCCTTGCTTGCTCTCACCAGCCCTGTTCCAGTCACGGGTGATCCCCGTGCTTCTCCAGTTGTTTGAAGTTCATGAAGAGCATGTGCGGATGGTGCTGCTGTCTCACATCGAGGCCTACGTGGAGCACTTCACTCAGGAGCAGCTGAAGAAAGTCATCTTGCCACAGGTTTTGCTGGGCATGCGTGATACTAGCGATTCCATTGTGGCAATTACTCTGCATAGCCTAGCAGTGCTGGTCTCTCTGCTTGGACCAGAGGTGGTTGTGGGAGGAGAACGAACCAAGATCTTCAAACGCACTGCCCCAAGTTTTACTAAAAATACTGACCTTTCTCTAGAAGGCGATCCATTTTCTCAGCCTATTAAATTTCCCATAAATGGACTCTCAGATGTAAAAAATACTTCGTAGGACAGTGAAAACTTCCCATCAAGTTCTAAAAAGTCTGAGGAGTGGCCTGACTGGAGTGAACCTGAGGAGCCTGAAAATCAAACTGTCAACATACAGATTTGGCCTAGAGAACCTTGTGATGATGTCAAGTCCCAGTGCACTACCTTGGATGTGGAAGAGTCATCTTGGGATGACTGCGAGCCCAGCAGCTTAGATACTAAAGTAAACCCAGGAGGTGGAATCACTGCTACAAAACCTGTTACCTCAGGGGAGCAGAAGCCTATTCCTGCTTTGCTTTCACTCACTGAAGAGTCTATGCCTTGGAAATCAAGCTTACCCCAAAAGATTAGCCTTGTACAAAGGGGGGATGACGCAGACCAAATCGAGCCGCCAAAAGTGTCATCACAAGAAAGGCCCCTTAAGGTTCCATCAGAACTTGGTTTAGGAGAGGAATTCACCATTCAAGTAAAAAAGAAGCCAGTAAAAGATCCTGAGATGGATTGGTTTGCTGATATGATCCCAGAAATTAAGCCTTCTGCTGCTTTTCTTATATTACCTGAACTGAGGACAGAAATGGTCCCAAAAAAGGATGATGTCTCCCCAGTGATGCAGTTTTCCTCAAAATTTGCTGCAGCAGAAATTACTGAGGGAGAGGCTGAAGGCTGGGAAGAAGAAGGGGAGCTGAACTGGGAAGATAATAACTGGTGA
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFDIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSATMYPYDVPDYAMGSENSALKSYTLREPPFTLPSGLAVYPAVLQDGKFASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLSCTVEADGIHLVTKRVQPLEVALETLSSAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKLGGMETVCKVSQATPEFLRSIQSIRDPASIPPEEMSPEFTTLPECHGHARDAFSFGTLVESLLTILNEQVSADVLSSFQQTLHSTLLNPIPKCRPALCTLLSHDFFRNDFLEVVNFLKSLTLKSEEEKTEFFKFLLDRVSCLSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKDHAQGETPCLLSPALFQSRVIPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGMRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEGDPFSQPIKFPINGLSDVKNTS*
Vector Type
Mammalian
Antibiotic
Kan
DU Number
DU2726
Unit Source
Location
PPU
MTA
Notes
Amplified from EST NCBI Acc. BI603430 IMAGE:5299347
Using primers :
1.GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGGATCAGAGAACAGTGCTTTAAAG
2.GCGGCCGCTCACCAGTTATTATCTTCCCAGTTCAGC
Compared to NCBI HS97P201 seq. this EST contained the following differences:
E83-is a K here
L385 is an M
There is a stop cod. At AA460
Using primers :
1.GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGGATCAGAGAACAGTGCTTTAAAG
2.GCGGCCGCTCACCAGTTATTATCTTCCCAGTTCAGC
Compared to NCBI HS97P201 seq. this EST contained the following differences:
E83-is a K here
L385 is an M
There is a stop cod. At AA460
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