cDNA Clone Tiam1 (mouse)

• Plasmid: pEBG6P-1 Tiam1 M393-F1511
• Parent Plasmid: pEBG6P
• Genbank: U05245.1
• Species: Mouse

Sequence of Insert

actagtATGAGTACCACCAACAGTGAGAGCCTGGAGGAGGCTGGCTCTGCGCACAGCGATGAGCAGAGCAGCGGAACCCTCAGTTCCCCGGGCCAGTCGGACATCCTGCTAACGGCTGCGCAGGGCACTGTGCGGAAGGCTGGAGCGCTGGCAGTCAAGAACTTCCTGGTCCACAAGAAGAATAAGAAGGTGGAGTCGGCTACCCGGAGGAAGTGGAAGCACTACTGGGTGTCCCTGAAAGGTTGCACGCTCTTTTTCTATGAGACTGATGGCAGGTCTGGGATAGACCACAACAGTGTCCCCAAG
CACGCCGTCTGGGTGGAGAACAGCATCGTGCAGGCTGTGCCTGAGCACCC
CAAGAAAGACTTCGTCTTCTGCCTCAGCAACTCCCTGGGTGACGCCTTCC
TCTTCCAGACCACGAGCCAGACAGAGCTGGAGAACTGGATCACCGCCATC
CACTCTGCCTGCGCAGCTGCCGTCGCAAGGCACCACCACAAGGAGGACAC
GCTCCGCCTCCTCAAGTCGGAAATCAAGAAGCTGGAGCAGAAGATCGACA
TGGACGAGAAGATGAAGAAGATGGGGGAGATGCAGCTGTCTTCCGTCACC
GACTCGAAGAAGAAGAAGACCATCCTAGACCAGATCTTCGTTTGGGAGCA
GAACCTCGAACAGTTCCAGATGGACCTGTTCCGATTCCGCTGTTACCTCG
CCAGCCTCCAGGGCGGGGAGCTGCCAAACCCCAAAAGGCTGCTTGCTTTT
GCGAGCCGGCCCACGAAGGTGGCCATGGGCCGCCTCGGGATCTTCTCCGT
GTCATCTTTTCATGCCCTGGTGGCAGCCCGGACTGGTGAGATCGGAGTGA
GAAGGCGTACTCAGGCCATGTCCAGGTCTGCAAGCAAGCGAAGGAGCAGG
TTTTCTTCTCTCTGGGGCCTAGACACTACCTCCAAAAAGAAGCAGGGACG
CCCAACCATCAACCAGGTGTTTGGAGAGGGGACTGATGCTGTAAAGAGAT
CTTTAGAGGGAATATTTGATGACACTGTTCCAGATGGCAAGAGGGAGAAG
GAAGTGGTCTTACCCAGTGTCCACCAGCACAACCCCGACTGTGACATTTG
GGTCCATGAATATTTCACTCCATCCTGGTTCTGTCTACCCAACAACCAGC
CAGCCTTGACGGTTGTCCGGCCAGGGGACACTGCGAGGGACACCTTGGAG
CTCATTTGCAAGACACATCAACTGGATCATTCCGCCCATTACCTGCGCCT
GAAATTCCTAATGGAGAACAGAGTGCAGTTCTACATCCCGCAGCCCGAGG
AGGACATTTACGAGCTGCTTTACAAAGAAATTGAAATCTGTCCAAAAGTC
ACCCAGAATATCCACATTGAGAAGTCAGACGCGGCCGCTGATAATTACGG
GTTTTTGCTTTCTTCTGTGGATGAAGATGGCATTCGAAGGCTCTACGTGA
ACAGTGTCAAGGAAACCGGGTTAGCTTCCAAGAAAGGCCTGAAGGCGGGG
GATGAGATTCTCGAGATCAATAATCGTGCTGCTGGTACCCTGAACTCATC
TATGCTCAAAGATTTCCTCTCACAGCCCTCCCTGGGCCTCCTGGTGAGGA
CCTACCCTGAGCCAGAGGGAGGCGTGGAGCTGCTGGAGAACCCTCCGCAC
CGAGTGGACGGCCCTGTGGACCTTGGCGAGAGCCCCCTCGCCTTCCTCAC
CAGCAACCCAGGGCACAGCCTCTCCAGTGAGCAGGGCAGCAGTGCTGAGA
CAGCTCCAGAAGAGGGCGAAGGACCGGACTTGGAGTCTTCTGATGAGACA
GATCACAGCAGCAAGAGCACAGAACAGGTTGCAGCGTTTTGCCGGAGTCT
GCACGAGATGAGCCCCTCTGACTCAAGCCCGTCCCCTCAGGATGCCACAA
GCCCTCAGCTGGCAACCACACGACAGCTGTCGGATGCGGATAAGCTGCGC
AAGGTGATCTGTGAACTGCTGGAGACTGAACGCACCTATGTGAAAGACTT
AAACTGCCTCATGGAGAGATACCTGAAGCCCCTTCAGAAGGAGACCTTTC
TCACCCAGGATGAGCTTGATGTACTTTTTGGAAATTTAACCGAAATGGTG
GAGTTTCAAGTCGAGTTCCTTAAGACTCTAGAAGATGGAGTAAGACTGGT
CCCTGACTTGGAAAAGCTGGAGAAGGTTGACCAGTTCAAGAAAGTGCTCT
TCTCTCTGGGGGGATCCTTCCTGTACTACGCGGACCGCTTCAAGCTCTAC
AGTGCCTTCTGTGCGAGCCACACAAAAGTCCCCAAGGTCCTGGTGAAAGC
CAAGACGGACACAGCCTTCAAGGCGTTCCTCGATGCCCAGAACCCGAGGC
AGCAGCACTCATCCACGCTGGAGTCTTACCTCATCAAGCCCATCCAGAGG
GTCCTCAAGTACCCGCTTCTGCTCAGGGAGCTGTTTGCGCTGACCGATGC
GGAGAGCGAGGAACACTACCACCTGGACGTGGCCATCAAGACTATGAACA
AGGTCGCCAGTCACATCAATGAGATGCAGAAGATCCACGAAGAGTTTGGT
GCTGTGTTTGACCAGCTGATTGCTGAGCAGACGGGAGAGAAGAAAGAGGT
TGCAGATCTGAGCATGGGTGACCTGCTCTTGCACACCAGCGTCATCTGGC
TGAACCCACCCGCCTCACTGGGAAAGTGGAAAAAGGAGCCAGAATTAGCA
GCCTTCGTCTTCAAAACGGCCGTGGTCCTTGTATATAAAGACGGTTCCAA
GCAGAAGAAGAAACTTGTTGGCTCTCACAGGCTGTCAATCTACGAGGAGT
GGGACCCTTTCCGGTTTCGCCACATGATCCCTACCGAAGCTTTGCAGGTC
CGAGCTCTGCCCAGTGCAGATGCAGAGGCAAATGCGGTATGCGAAATTGT
CCACGTGAAATCAGAGTCAGAAGGGAGGCCCGAGCGGGTTTTCCACCTCT
GCTGCAGTTCCCCAGAGAGCAGAAAGGACTTTCTGAAGTCTGTGCATTCG
ATCCTGCGAGATAAACACAGAAGACAGCTCCTCAAAACGGAAAGCCTTCC
GTCAGCCCAGCAGTATGTCCCCTTTGGAGGCAAGAGATTGTGTGCGCTTA
AAGGCGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGCCCCAAGCAAGTCT
CTTGGGAGGAGGAGGCGGCGACTGGCCCGAAACAGGTTTACCATCGATTC
AGACGCCATCTCAGCCAGCAGCCCGGAGAAAGAGCCCCAGCAGCCCGCCG
GTGGTGGGGACACTGACCGATGGGTAGAGGAACAGTTCGATCTTGCTCAG
TACGAGGAGCAGGATGACATCAAGGAGACAGACATCCTCAGTGACGATGA
CGAATTCTGTAGACTAGT

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMSTTNSESLEE
AGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKK
VESATRRKWKHYWVSLKGCTLFFYETDGRSGIDHNSVPKHAVWVENSIVQ
AVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARH
HHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQ
IFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLAFASRPTKVAMGR
LGIFSVSSFHALVAARTGEIGVRRRTQAMSRSASKRRSRFSSLWGLDTTS
KKKQGRPTINQVFGEGTDAVKRSLEGIFDDTVPDGKREKEVVLPSVHQHN
PDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTHQLDHS
AHYLRLKFLMENRVQFYIPQPEEDIYELLYKEIEICPKVTQNIHIEKSDA
AADNYGFLLSSVDEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAA
GTLNSSMLKDFLSQPSLGLLVRTYPEPEGGVELLENPPHRVDGPVDLGES
PLAFLTSNPGHSLSSEQGSSAETAPEEGEGPDLESSDETDHSSKSTEQVA
AFCRSLHEMSPSDSSPSPQDATSPQLATTRQLSDADKLRKVICELLETER
TYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLE
DGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVP
KVLVKAKTDTAFKAFLDAQNPRQQHSSTLESYLIKPIQRVLKYPLLLREL
FALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQT
GEKKEVADLSMGDLLLHTSVIWLNPPASLGKWKKEPELAAFVFKTAVVLV
YKDGSKQKKKLVGSHRLSIYEEWDPFRFRHMIPTEALQVRALPSADAEAN
AVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKSVHSILRDKHRRQLL
KTESLPSAQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARN
RFTIDSDAISASSPEKEPQQPAGGGDTDRWVEEQFDLAQYEEQDDIKETD
ILSDDDEFCRLVVPSMRPRL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11195
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Tiam1 M393-F1511 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 154988 Daltons