cDNA Clone PTIP

• Plasmid: pCMV5-FLAG PTIP S2-N1069(end) W676A
• Parent Plasmid: pCMV5
• Expressed: FLAG PTIP S2-N1069(end) W676A
• Genbank: NM_007349.3 GI:93141032 with 4 silent changes
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCGCCACCATGGACTACAAAGACGATGACGATAAATCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGGTCAAGTATTACGCGGTGGGCGACATCGACCCGCAGGTTATTCAGCTTCTCAAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACATAATCTCAGAGGATGGGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCTTTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGTGGAACTCTTCTGCCAGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTTTTTTGGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTGCCCTGTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTCAATAAGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATACGAATGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGGTTCTGGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCATCCTCGTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGAGGAAGTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATGAGAAGTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAGCAGTTTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTTTGATGATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACTGGACCCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCTCAGGGAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGTCCCAGGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTGGACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATGAACCGGCCATCAAATGTAGCACATATCCTACAGACTCTCTCAGCACCTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGGTTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAGCAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTCACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGCAACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCATCGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCAGCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCCCAGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATTCAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGATGCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGCATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCACCACCATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGAGATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACGCGTTAAACACAGTCTTAAAGAAGAAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGAACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTGAGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTCCCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAGCAAGGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCATATAAGTTTAACTGAAAGCTT

Amino Acid Sequence

MDYKDDDDKSDQAPKVPEEMFREVKYYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEAREVFDLPVVKPSWVILSVQCGTLLPVNGFS
PESCQIFFGITACLSQVSSEDRSALWALVTFYGGDCQLTLNKKCTHLIVPEPKGEKYECALKRASIKIVTPDWVLDCVSEKTKKDEAFYHPRLIIYEEEE
EEEEEEEEVENEEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEPGLINL
CANVPPVPGNILPPEVRGNLMAAGQNLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAVLFSQV
KVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQLQPFQ
QQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQQHQLF
GHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRERKRCVTAHALNTVLKKKKMVPPHRA
LHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYT
AFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGE
VAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMK
AIVECAGGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee