cDNA Clone SIK

• Plasmid: pCMV5-HA SIK
• Parent Plasmid: pCMV5
• Expressed: HA-SIK
• Genbank: NM_173354
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCGTTATCAT
GTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGC
CCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAAC
TTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGC
AATAAAAATAATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAA
TCTATCGTGAGGTTCAGCTGATGAAGCTTCTGAACCATCCACACATCATA
AAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTACATCGTCACTGA
ATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTCCAACGGGCACC
TGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGCCGTG
GAGTACTGTCACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAA
CCTCCTGCTGGATGGCAACATGGACATCAAGCTGGCAGATTTTGGATTTG
GGAATTTCTACAAGTCAGGAGAGCCTCTGTCCACGTGGTGTGGGAGCCCC
CCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAGGAGTATGAAGGCCCCCA
GCTGGACATCTGGAGCCTGGGCGTGGTGCTGTACGTCCTGGTCTGCGGTT
CTCTCCCCTTCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGTGCTG
GAGGGCCGCTTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCT
GATCCGCCGCATGCTGGTGGTGGACCCCGCCAGGCGCATCACCATCGCCC
AGATCCGGCAGCACCGGTGGATGCGGGCTGAGCCCTGCTTGCCGGGACCC
GCCTGCCCCGCCTTCTCCGCACACAGCTACACCTCCAACCTGGGCGACTA
CGATGAGCAGGCGCTGGGTATCATGCAGACCCTGGGCGTGGACCGGCAGA
GGACGGTGGAGTCACTGCAAAACAGCAGCTATAACCACTTTGCTGCCATT
TATTACCTCCTCCTTGAGCGGCTCAAGGAGTATCGGAATGCCCAGTGCGC
CCGCCCCGGGCCTGCCAGGCAGCCGCGGCCTCGGAGCTCGGACCTCAGTG
GTTTGGAGGTGCCTCAGGAAGGTCTTTCCACCGACCCTTTCCGACCTGCC
TTGCTGTGCCCGCAGCCGCAGACCTTGGTGCAGTCCGTCCTCCAGGCCGA
GATGGACTGTGAGCTCCAGAGCTCGCTGCAGTGGCCCTTGTTCTTCCCGG
TGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCCGGCCCGTGTCCCCAAGC
AGCCTGCTGGACACAGCCATCAGTGAGGAGGCCAGGCAGGGGCCGGGCCT
AGAGGAGGAGCAGGACACGCAGGAGTCCCTGCCCAGCAGCACGGGCCGGA
GGCACACCCTGGCCGAGGTCTCCACCCGCCTCTCCCCACTCACCGCGCCA
TGTATAGTCGTCTCCCCCTCCACCACGGCAAGTCCTGCAGAGGGAACCAG
CTCTGACAGTTGTCTGACCTTCTCTGCGAGCAAAAGCCCCGCGGGGCTCA
GTGGCACCCCGGCCACTCAGGGGCTGCTGGGCGCCTGCTCCCCGGTCAGG
CTGGCCTCGCCCTTCCTGGGGTCGCAGTCCGCCACCCCAGTGCTGCAGGC
TCAGGGGGGCTTGGGAGGAGCTGTTCTGCTCCCTGTCAGCTTCCAGGAGG
GACGGCGGGCGTCGGACACCTCACTGACTCAAGGGCTGAAGGCCTTTCGG
CAGCAGCTGAGGAAGACCACGCGGACCAAAGGGTTTCTGGGACTGAACAA
AATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGTCCCTGCCAGCCGGGCCA
GCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTGCACAGAGCCCAGGCCTG
CACGGCGGCGCAGCCGGCAGCCGGGAGGGCTGGAGCCTGCTGGAGGAGGT
GCTAGAGCAGCAGAGGCTGCTCCAGTTACAGCACCACCCGGCCGCTGCAC
CCGGCTGCTCCCAGGCCCCCCAGCCGGCCCCTGCCCCGTTTGTGATCGCC
CCCTGTGATGGCCCTGGGGCTGCCCCGCTCCCCAGCACCCTCCTCACGTC
GGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCAGACCGGCGCGTCCCCGG
TGGCCTCAGCGGCGCAGCTCCTGGACACACACCTGCACATTGGCACCGGC
CCCACCGCCCTCCCCGCTGTGCCCCCACCACGCCTGGCCAGGCTGGCCCC
AGGTTGTGAGCCCCTGGGGCTGCTGCAGGGGGACTGTGAGATGGAGGACC
TGATGCCCTGCTCCCTAGGCACGTTTGTCCTGGTGCAGTGAggatcc

Amino Acid Sequence

MYPYDVPDYAVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVV
KLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPHIIKLYQ
VMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCH
DHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKSGEPLSTWCGSPPYAA
PEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRF
RIPFFMSQDCESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPA
FSAHSYTSNLGDYDEQALGIMQTLGVDRQRTVESLQNSSYNHFAAIYYLL
LERLKEYRNAQCARPGPARQPRPRSSDLSGLEVPQEGLSTDPFRPALLCP
QPQTLVQSVLQAEMDCELQSSLQWPLFFPVDASCSGVFRPRPVSPSSLLD
TAISEEARQGPGLEEEQDTQESLPSSTGRRHTLAEVSTRLSPLTAPCIVV
SPSTTASPAEGTSSDSCLTFSASKSPAGLSGTPATQGLLGACSPVRLASP
FLGSQSATPVLQAQGGLGGAVLLPVSFQEGRRASDTSLTQGLKAFRQQLR
KTTRTKGFLGLNKIKGLARQVCQVPASRASRGGLSPFHAPAQSPGLHGGA
AGSREGWSLLEEVLEQQRLLQLQHHPAAAPGCSQAPQPAPAPFVIAPCDG
PGAAPLPSTLLTSGLPLLPPPLLQTGASPVASAAQLLDTHLHIGTGPTAL
PAVPPPRLARLAPGCEPLGLLQGDCEMEDLMPCSLGTFVLVQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5-HA SIK58.24 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct to express SIK with N-terminal HA tag.
• Low Temp: No