cDNA Clone TRB3

• Plasmid: pGEX6P-1-TRB3
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRB3
• Genbank: NM_021158
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAGGAA
GAAGCGGTTGGAGTTGGATGACAACTTAGATACCGAGCGTCCCGTCCAGA
AACGAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGCCTGTTGCCC
CTGAGCCCACCTACTGCTCCAGATCGTGCAACTGCTGTGGCCACTGCCTC
CCGTCTTGGGCCCTATGTCCTCCTGGAGCCCGAGGAGGGCGGGCGGGCCT
ACCAGGCCCTGCACTGCCCTACAGGCACTGAGTATACCTGCAAGGTGTAC
CCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTATGCGCGGCTGCCCCC
GCACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCC
TCTACGCCTTTTTCACTCGGACCCATGGGGACATGCACAGCCTGGTGCGA
AGCCGCCACCGTATCCCTGAGCCTGAGGCTGCCGTGCTCTTCCGCCAGAT
GGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTGCGTGATC
TCAAGCTGTGTCGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTGGTG
CTGGAGAACCTGGAGGACTCCTGCGTGCTGACTGGGCCAGATGATTCCCT
GTGGGACAAGCACGCGTGCCCAGCCTACGTGGGACCTGAGATACTCAGCT
CACGGGCCTCATACTCGGGCAAGGCAGCCGATGTCTGGAGCCTGGGCGTG
GCGCTCTTCACCATGCTGGCCGGCCACTACCCCTTCCAGGACTCGGAGCC
TGTCCTGCTCTTCGGCAAGATCCGCCGCGGGGCCTACGCCTTGCCTGCAG
GCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGGGAG
CCAGCTGAACGGCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCTGCG
ACAGGACCCGATGCCCTTAGCCCCAACCCGATCCCATCTCTGGGAGGCTG
CCCAGGTGGTCCCTGATGGACTGGGGCTGGACGAAGCCAGGGAAGAGGAG
GGAGACAGAGAAGTGGTTCTGTATGGCTAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYQALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion