cDNA Clone PGC-1

• Plasmid: pEBG-2T-PGC-1
• Parent Plasmid: pEBG2T
• Expressed: GST-PGC-1
• Genbank: NM_013261
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGCGTGGGACATGTGCAACCAGGACTCTGAGTCTGTATGGAGTGA
CATCGAGTGTGCTGCTCTGGTTGGTGAAGACCAGCCTCTTTGCCCAGATC
TTCCTGAACTTGATCTTTCTGAACTAGATGTGAACGACTTGGATACAGAC
AGCTTTCTGGGTGGACTCAAGTGGTGCAGTGACCAATCAGAAATAATATC
CAATCAGTACAACAATGAGCCTTCAAACATATTTGAGAAGATAGATGAAG
AGAATGAGGCAAACTTGCTAGCAGTCCTCACAGAGACACTAGACAGTCTC
CCTGTGGATGAAGACGGATTGCCCTCATTTGATGCGCTGACAGATGGAGA
CGTGACCACTGACAATGAGGCTAGTCCTTCCTCCATGCCTGACGGCACCC
CTCCACCCCAGGAGGCAGAAGAGCCGTCTCTACTTAAGAAGCTCTTACTG
GCACCAGCCAACACTCAGCTAAGTTATAATGAATGCAGTGGTCTCAGTAC
CCAGAACCATGCAAATCACAATCACAGGATCAGAACAAACCCTGCAATTG
TTAAGACTGAGAATTCATGGAGCAATAAAGCGAAGAGTATTTGTCAACAG
CAAAAGCCACAAAGACGTCCCTGCTCGGAGCTTCTCAAATATCTGACCAC
AAACGATGACCCTCCTCACACCAAACCCACAGAGAACAGAAACAGCAGCA
GAGACAAATGCACCTCCAAAAAGAAGTCCCACACACAGTCGCAGTCACAA
CACTTACAAGCCAAACCAACAACTTTATCTCTTCCTCTGACCCCAGAGTC
ACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATTGAACGCA
CCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCACCCACCACT
CCTCCTCATAAAGCCAACCAAGATAACCCTTTTAGGGCTTCTCCAAAGCT
GAAGTCCTCTTGCAAGACTGTGGTGCCACCACCATCAAAGAAGCCCAGGT
ACAGTGAGTCTTCTGGTACACAAGGCAATAACTCCACCAAGAAAGGGCCG
GAGCAATCCGAGTTGTATGCACAACTCAGCAAGTCCTCAGTCCTCACTGG
TGGACACGAGGAAAGGAAGACCAAGCGGCCCAGTCTGCGGCTGTTTGGTG
ACCATGACTATTGCCAGTCAATTAATTCCAAAACGGAAATACTCATTAAT
ATATCACAGGAGCTCCAAGACTCTAGACAACTAGAAAATAAAGATGTCTC
CTCTGATTGGCAGGGGCAGATTTGTTCTTCCACAGATTCAGACCAGTGCT
ACCTGAGAGAGACTTTGGAGGCAAGCAAGCAGGTCTCTCCTTGCAGCACA
AGAAAACAGCTCCAAGACCAGGAAATCCGAGCCGAGCTGAACAAGCACTT
CGGTCATCCCAGTCAAGCTGTTTTTGACGACGAAGCAGACAAGACCGGTG
AACTGAGGGACAGTGATTTCAGTAATGAACAATTCTCCAAACTACCTATG
TTTATAAATTCAGGACTAGCCATGGATGGCCTGTTTGATGACAGCGAAGA
TGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACGCAATCCTATTCAT
TGTTCAATGTGTCTCCTTCTTGTTCTTCTTTTAACTCTCCATGTAGAGAT
TCTGTGTCACCACCCAAATCCTTATTTTCTCAAAGACCCCAAAGGATGCG
CTCTCGTTCAAGGTCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCAT
ATTCCAGGTCAAGATCAAGGTCTCCAGGCAGTAGATCCTCTTCAAGATCC
TGCTATTACTATGAGTCAAGCCACTACAGACACCGCACGCACCGAAATTC
TCCCTTGTATGTGAGATCACGTTCAAGATCGCCCTACAGCCGTCGGCCCA
GGTATGACAGCTACGAGGAATATCAGCACGAGAGGCTGAAGAGGGAAGAA
TATCGCAGAGAGTATGAGAAGCGAGAGTCTGAGAGGGCCAAGCAAAGGGA
GAGGCAGAGGCAGAAGGCAATTGAAGAGCGCCGTGTGATTTATGTCGGTA
AAATCAGACCTGACACAACACGGACAGAACTGAGGGACCGTTTTGAAGTT
TTTGGTGAAATTGAGGAGTGCACAGTAAATCTGCGGGATGATGGAGACAG
CTATGGTTTCATTACCTACCGTTATACCTGTGATGCTTTTGCTGCTCTTG
AAAATGGATACACTTTGCGCAGGTCAAACGAAACTGACTTTGAGCTGTAC
TTTTGTGGACGCAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATTC
AAACTCAGATGACTTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTC
TGGATTTTGATAGTTTACTGAAAGAAGCTCAGAGAAGCTTGCGCAGGTAA
gcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1092
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag; Change to NM_013261 ORF sequence: ; a1584g, silent in T528