cDNA Clone SIK

• Plasmid: pEBG-2T-HA SIK
• Parent Plasmid: pEBG2T
• Expressed: GST-HA-SIK
• Genbank: NM_173354
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTACCCATACGATGTGCCAGATTACGCCGTTATCATGTCGGAGTT
CAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGCCCCTCCGGG
TGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAACTTCGCGGTG
GTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAAAAAT
AATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTG
AGGTTCAGCTGATGAAGCTTCTGAACCATCCACACATCATAAAGCTTTAC
CAGGTTATGGAAACAAAGGACATGCTTTACATCGTCACTGAATTTGCTAA
AAATGGAGAAATGTTTGATTATTTGACTTCCAACGGGCACCTGAGTGAGA
ACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGCCGTGGAGTACTGT
CACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAACCTCCTGCT
GGATGGCAACATGGACATCAAGCTGGCAGATTTTGGATTTGGGAATTTCT
ACAAGTCAGGAGAGCCTCTGTCCACGTGGTGTGGGAGCCCCCCGTATGCC
GCCCCGGAAGTCTTTGAGGGGAAGGAGTATGAAGGCCCCCAGCTGGACAT
CTGGAGCCTGGGCGTGGTGCTGTACGTCCTGGTCTGCGGTTCTCTCCCCT
TCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGTGCTGGAGGGCCGC
TTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCTGATCCGCCG
CATGCTGGTGGTGGACCCCGCCAGGCGCATCACCATCGCCCAGATCCGGC
AGCACCGGTGGATGCGGGCTGAGCCCTGCTTGCCGGGACCCGCCTGCCCC
GCCTTCTCCGCACACAGCTACACCTCCAACCTGGGCGACTACGATGAGCA
GGCGCTGGGTATCATGCAGACCCTGGGCGTGGACCGGCAGAGGACGGTGG
AGTCACTGCAAAACAGCAGCTATAACCACTTTGCTGCCATTTATTACCTC
CTCCTTGAGCGGCTCAAGGAGTATCGGAATGCCCAGTGCGCCCGCCCCGG
GCCTGCCAGGCAGCCGCGGCCTCGGAGCTCGGACCTCAGTGGTTTGGAGG
TGCCTCAGGAAGGTCTTTCCACCGACCCTTTCCGACCTGCCTTGCTGTGC
CCGCAGCCGCAGACCTTGGTGCAGTCCGTCCTCCAGGCCGAGATGGACTG
TGAGCTCCAGAGCTCGCTGCAGTGGCCCTTGTTCTTCCCGGTGGATGCCA
GCTGCAGCGGAGTGTTCCGGCCCCGGCCCGTGTCCCCAAGCAGCCTGCTG
GACACAGCCATCAGTGAGGAGGCCAGGCAGGGGCCGGGCCTAGAGGAGGA
GCAGGACACGCAGGAGTCCCTGCCCAGCAGCACGGGCCGGAGGCACACCC
TGGCCGAGGTCTCCACCCGCCTCTCCCCACTCACCGCGCCATGTATAGTC
GTCTCCCCCTCCACCACGGCAAGTCCTGCAGAGGGAACCAGCTCTGACAG
TTGTCTGACCTTCTCTGCGAGCAAAAGCCCCGCGGGGCTCAGTGGCACCC
CGGCCACTCAGGGGCTGCTGGGCGCCTGCTCCCCGGTCAGGCTGGCCTCG
CCCTTCCTGGGGTCGCAGTCCGCCACCCCAGTGCTGCAGGCTCAGGGGGG
CTTGGGAGGAGCTGTTCTGCTCCCTGTCAGCTTCCAGGAGGGACGGCGGG
CGTCGGACACCTCACTGACTCAAGGGCTGAAGGCCTTTCGGCAGCAGCTG
AGGAAGACCACGCGGACCAAAGGGTTTCTGGGACTGAACAAAATCAAGGG
GCTGGCTCGCCAGGTGTGCCAGGTCCCTGCCAGCCGGGCCAGCAGGGGCG
GCCTGAGCCCCTTCCACGCCCCTGCACAGAGCCCAGGCCTGCACGGCGGC
GCAGCCGGCAGCCGGGAGGGCTGGAGCCTGCTGGAGGAGGTGCTAGAGCA
GCAGAGGCTGCTCCAGTTACAGCACCACCCGGCCGCTGCACCCGGCTGCT
CCCAGGCCCCCCAGCCGGCCCCTGCCCCGTTTGTGATCGCCCCCTGTGAT
GGCCCTGGGGCTGCCCCGCTCCCCAGCACCCTCCTCACGTCGGGGCTCCC
GCTGCTGCCGCCCCCACTCCTGCAGACCGGCGCGTCCCCGGTGGCCTCAG
CGGCGCAGCTCCTGGACACACACCTGCACATTGGCACCGGCCCCACCGCC
CTCCCCGCTGTGCCCCCACCACGCCTGGCCAGGCTGGCCCCAGGTTGTGA
GCCCCTGGGGCTGCTGCAGGGGGACTGTGAGATGGAGGACCTGATGCCCT
GCTCCCTAGGCACGTTTGTCCTGGTGCAGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSYPYDVPDYAVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKSGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSNLGDYDEQALGIMQTLGVDRQRTVESLQNSSYNHFAAIYYLLLERLKEYRNAQCARPGPARQPRPRSSDLSGLEVPQEGLSTDPFRPALLCPQPQTLVQSVLQAEMDCELQSSLQWPLFFPVDASCSGVFRPRPVSPSSLLDTAISEEARQGPGLEEEQDTQESLPSSTGRRHTLAEVSTRLSPLTAPCIVVSPSTTASPAEGTSSDSCLTFSASKSPAGLSGTPATQGLLGACSPVRLASPFLGSQSATPVLQAQGGLGGAVLLPVSFQEGRRASDTSLTQGLKAFRQQLRKTTRTKGFLGLNKIKGLARQVCQVPASRASRGGLSPFHAPAQSPGLHGGAAGSREGWSLLEEVLEQQRLLQLQHHPAAAPGCSQAPQPAPAPFVIAPCDGPGAAPLPSTLLTSGLPLLPPPLLQTGASPVASAAQLLDTHLHIGTGPTALPAVPPPRLARLAPGCEPLGLLQGDCEMEDLMPCSLGTFVLVQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1081
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express SIK with N terminal GST and HA tags