cDNA Clone SACM1L

• Plasmid: pEBG-2T-SACM1L
• Parent Plasmid: pEBG2T
• Expressed: GST-SACM1L
• Genbank: NM_014016
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGACGGCGGCCTACGAGCAGCTGAAGCTGCATATCACACCTGA
AAAATTTTATGTGGAAGCTTGTGATGATGGAGCAGATGACGTACTTACCA
TTGACCGTGTGTCCACAGAGGTTACCCTTGCAGTCAAGAAAGATGTTCCT
CCTTCAGCTGTCACAAGACCAATATTTGGTATACTGGGCACAATCCATCT
GGTGGCAGGTAATTATCTTATAGTCATTACCAAAAAGATAAAAGTAGGTG
AATTTTTCAGTCATGTAGTCTGGAAAGCAACAGATTTTGATGTCCTTTCT
TATAAGAAGACAATGTTGCACTTAACTGATATTCAGTTACAAGATAATAA
AACCTTCCTAGCGATGCTAAACCATGTCTTGAATGTGGATGGATTTTACT
TTTCAACAACATATGATTTGACCCATACTTTGCAGCGGCTATCCAACACT
AGTCCTGAATTCCAAGAAATGAGTCTCTTGGAAAGGGCAGATCAGCGGTT
TGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCAGAGGTTC
ATCGGTTTGCCCTTCCAGTGTTACATGGCTTTATTACCATGCATTCATGT
TCTATTAATGGAAAATACTTTGATTGGATTCTCATCTCGAGGAGGAGCTG
TTTCAGAGCTGGTGTGCGCTATTATGTAAGAGGAATTGATTCGGAAGGCC
ATGCAGCTAACTTTGTAGAAACAGAACAAATTGTGCACTACAATGGGAGC
AAAGCTTCGTTTGTACAGACTCGAGGATCAATACCTGTTTTCTGGTCCCA
AAGACCAAACCTCAAGTACAAACCACTGCCACAGATCAGCAAAGTAGCAA
ATCACATGGACGGTTTCCAAAGGCATTTTGATTCCCAAGTAATTATTTAT
GGAAAACAAGTTATAATCAATCTGATTAACCAGAAGGGCTCGGAGAAGCC
ACTTGAGCAGACATTTGCAACAATGGTGTCTTCCTTGGGAAGTGGAATGA
TGAGATACATTGCCTTTGACTTCCATAAGGAATGTAAAAATATGAGATGG
GATCGACTAAGTATTTTATTGGATCAGGTAGCAGAAATGCAAGATGAATT
AAGTTATTTTCTAGTGGACTCTGCTGGCCAGGTGGTGGCAAACCAGGAAG
GCGTGTTCCGAAGCAATTGCATGGATTGTCTAGATAGAACCAATGTGATC
CAGAGTTTGTTAGCTCGTCGTTCACTTCAGGCCCAACTTCAGAGACTAGG
AGTTTTGCATGTGGGACAAAAGCTTGAAGAACAAGATGAATTTGAGAAGA
TTTTCAAAAATGCCTGGGCTGACAACGCAAATGCTTGTGCCAAGCAATAT
GCGGGAACTGGTGCCTTGAAGACTGACTTTACCAGAACTGGAAAGAGAAC
TCATTTGGGACTTATAATGGATGGCTGGAACTCAATGATACGATATTATA
AGAACAACTTTTCCGATGGATTTAGACAAGATTCCATAGACTTATTTCTT
GGAAACTATTCAGTGGATGAATTAGAATCTCATAGTCCTTTAAGTGTTCC
AAGGGACTGGAAATTCCTGGCTTTGCCTATTATCATGGTTGTTGCCTTTT
CAATGTGCATTATCTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACA
CTGGCCTATGTGCTCTTCTGGGGAGTTGCAAGCATTGGAACATTTTTTAT
CATTCTTTACAATGGCAAAGATTTTGTCGATGCTCCCAGACTGGTCCAGA
AAGAAAAGATAGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIFKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1067
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tagChange to NM_014016 ORF sequence: ; a1301t leading to Y434F. ; This change present in many EST sequences for SACM1L – natural polymorphism.