cDNA Clone PYK2

• Plasmid: pCMV5 PYK2
• Parent Plasmid: pCMV5
• Expressed: PYK2 (no tag)
• Genbank: HSU33284
• Tag: untagged

Sequence of Insert

gaattcatgtctggggtgtccgagcccctgagtcgagtaaagttgggcac
gttacgccggcctgaaggccctgcagagcccatggtggtggtaccagtag
atgtggaaaaggaggacgtgcgtatcctcaaggtctgcttctatagcaac
agcttcaatcctgggaaaaacttcaaactggtcaaatgcactgtccagac
ggagatccgggagatcatcacctccatcctgctgagcgggcggatcgggc
ccaacatccggttggctgagtgctatgggctgaggctgaagcacatgaag
tccgatgagatccactggctgcacccacagatgacagtgggtgaggtgca
ggacaagtatgagtgtctgcacgtggaagccgagtggaggtatgaccttc
aaatccgctacttgccagaagacttcatggagagcctgaaggaggacagg
accacgctgctctatttttaccaacagctccggaacgactacatgcagcg
ctacgccagcaaggtcagcgagggcatggccctgcagctgggctgcctgg
agctcaggcggttcttcaaggatatgccccacaatgcacttgacaagaag
tccaacttcgagctcctagaaaaggaagtggggctggacttgtttttccc
aaagcagatgcaggagaacttaaagcccaaacagttccggaagatgatcc
agcagaccttccagcagtacgcctcgctcagggaggaggagtgcgtcatg
aagttcttcaacactctcgccggcttcgccaacatcgaccaggagaccta
ccgctgtgaactcattcaaggatggaacattactgtggacctggtcattg
gccctaaagggatccgccagctgactagtcaggacgcaaagcccacctgc
ctggccgagttcaagcagatcaggtccatcaggtgcctcccgctggagga
gggccaggcagtacttcagctgggcattgaaggtgccccccaggccttgt
ccatcaaaacctcatccctagcagaggctgagaacatggctgacctcata
gacggctactgccggctgcagggtgagcaccaaggctctctcatcatcca
tcctaggaaagatggtgagaagcggaacagcctgccccagatccccatgc
taaacctggaggcccggcggtcccacctctcagagagctgcagcatagag
tcagacatctacgcagagattcccgacgaaaccctgcgaaggcccggagg
tccacagtatggcattgcccgtgaagatgtggtcctgaatcgtattcttg
gggaaggcttttttggggaggtctatgaaggtgtctacacaaatcataaa
ggggagaaaatcaatgtagctgtcaagacctgcaagaaagactgcactct
ggacaacaaggagaagttcatgagcgaggcagtgatcatgaagaacctcg
accacccgcacatcgtgaagctgatcggcatcattgaagaggagcccacc
tggatcatcatggaattgtatccctatggggagctgggccactacctgga
gcggaacaagaactccctgaaggtgctcaccctcgtgctgtactcactgc
agatatgcaaagccatggcctacctggagagcatcaactgcgtgcacagg
gacattgctgtccggaacatcctggtggcctcccctgagtgtgtgaagct
gggggactttggtctttcccggtacattgaggacgaggactattacaaag
cctctgtgactcgtctccccatcaaatggatgtccccagagtccattaac
ttccgacgcttcacgacagccagtgacgtctggatgttcgccgtgtgcat
gtgggagatcctgagctttgggaagcagcccttcttctggctggagaaca
aggatgtcatcggggtgctggagaaaggagaccggctgcccaagcctgat
ctctgtccaccggtcctttataccctcatgacccgctgctgggactacga
ccccagtgaccggccccgcttcaccgagctggtgtgcagcctcagtgacg
tttatcagatggagaaggacattgccatggagcaagagaggaatgctcgc
taccgaacccccaaaatcttggagcccacagccttccaggaacccccacc
caagccgagccgacctaagtacagaccccctccgcaaaccaacctcctgg
ctccaaagctgcagttccaggttcctgagggtctgtgtgccagctctcct
acgctcaccagccctatggagtatccatctcccgttaactcactgcacac
cccacctctccaccggcacaatgtcttcaaacgccacagcatgcgggagg
aggacttcatccaacccagcagccgagaagaggcccagcagctgtgggag
gctgaaaaggtcaaaatgcggcaaatcctggacaaacagcagaagcagat
ggtggaggactaccagtggctcaggcaggaggagaagtccctggacccca
tggtttatatgaatgataagtccccattgacgccagagaaggaggtcggc
tacctggagttcacagggcccccacagaagcccccgaggctgggcgcaca
gtccatccagcccacagctaacctggaccggaccgatgacctggtgtacc
tcaatgtcatggagctggtgcgggccgtgctggagctcaagaatgagctc
tgtcagctgccccccgagggctacgtggtggtggtgaagaatgtggggct
gaccctgcggaagctcatcgggagcgtggatgatctcctgccttccttgc
cgtcatcttcacggacagagatcgagggcacccagaaactgctcaacaaa
gacctggcagagctcatcaacaagatgcggctggcgcagcagaacgccgt
gacctccctgagtgaggagtgcaagaggcagatgctgacggcttcacaca
ccctggctgtggacgccaagaacctgctcgacgctgtggaccaggccaag
gttctggccaatctggcccacccacctgcagagtgatctaga

Amino Acid Sequence

MSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSF
NPGKNFKLVKCTVQTEIREIITSILLSGRIGPNIRLAECYGLRLKHMKSD
EIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTT
LLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSN
FELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKF
FNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDAKPTCLA
EFKQIRSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDG
YCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRSHLSESCSIESD
IYAEIPDETLRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGVYTNHKGE
KINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWI
IMELYPYGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDI
AVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFR
RFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLC
PPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYR
TPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTL
TSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAE
KVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYL
EFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQ
LPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDL
AELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVL
ANLAHPPAE*

• Vector Type: Mammalian
• DU Number: DU1020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This clone contains a number of silent nucleotide changes that differ from the published sequence and are also present in all available ESTs: c27t, a45g, g162a, g330a, c1341t, c2151g (counting from the first nucleotide of PYK2 ORF).