cDNA Clone PYK2

• Synonyms: PKB; PTK; CAKB; FAK2; PTK2B; CADTK; FADK2; RAFTK
• Plasmid: pFastBac.HTa PYK2
• Parent Plasmid: pSC-A
• Expressed: 6His-PYK2
• Genbank: NM_173174.2
• Tag: 6His
• Species: Human

Sequence of Insert

gaattctctggggtgtccgagcccctgagtcgagtaaagttgggcacgtt
acgccggcctgaaggccctgcagagcccatggtggtggtaccagtagatg
tggaaaaggaggacgtgcgtatcctcaaggtctgcttctatagcaacagc
ttcaatcctgggaaaaacttcaaactggtcaaatgcactgtccagacgga
gatccgggagatcatcacctccatcctgctgagcgggcggatcgggccca
acatccggttggctgagtgctatgggctgaggctgaagcacatgaagtcc
gatgagatccactggctgcacccacagatgacagtgggtgaggtgcagga
caagtatgagtgtctgcacgtggaagccgagtggaggtatgaccttcaaa
tccgctacttgccagaagacttcatggagagcctgaaggaggacaggacc
acgctgctctatttttaccaacagctccggaacgactacatgcagcgcta
cgccagcaaggtcagcgagggcatggccctgcagctgggctgcctggagc
tcaggcggttcttcaaggatatgccccacaatgcacttgacaagaagtcc
aacttcgagctcctagaaaaggaagtggggctggacttgtttttcccaaa
gcagatgcaggagaacttaaagcccaaacagttccggaagatgatccagc
agaccttccagcagtacgcctcgctcagggaggaggagtgcgtcatgaag
ttcttcaacactctcgccggcttcgccaacatcgaccaggagacctaccg
ctgtgaactcattcaaggatggaacattactgtggacctggtcattggcc
ctaaagggatccgccagctgactagtcaggacgcaaagcccacctgcctg
gccgagttcaagcagatcaggtccatcaggtgcctcccgctggaggaggg
ccaggcagtacttcagctgggcattgaaggtgccccccaggccttgtcca
tcaaaacctcatccctagcagaggctgagaacatggctgacctcatagac
ggctactgccggctgcagggtgagcaccaaggctctctcatcatccatcc
taggaaagatggtgagaagcggaacagcctgccccagatccccatgctaa
acctggaggcccggcggtcccacctctcagagagctgcagcatagagtca
gacatctacgcagagattcccgacgaaaccctgcgaaggcccggaggtcc
acagtatggcattgcccgtgaagatgtggtcctgaatcgtattcttgggg
aaggcttttttggggaggtctatgaaggtgtctacacaaatcataaaggg
gagaaaatcaatgtagctgtcaagacctgcaagaaagactgcactctgga
caacaaggagaagttcatgagcgaggcagtgatcatgaagaacctcgacc
acccgcacatcgtgaagctgatcggcatcattgaagaggagcccacctgg
atcatcatggaattgtatccctatggggagctgggccactacctggagcg
gaacaagaactccctgaaggtgctcaccctcgtgctgtactcactgcaga
tatgcaaagccatggcctacctggagagcatcaactgcgtgcacagggac
attgctgtccggaacatcctggtggcctcccctgagtgtgtgaagctggg
ggactttggtctttcccggtacattgaggacgaggactattacaaagcct
ctgtgactcgtctccccatcaaatggatgtccccagagtccattaacttc
cgacgcttcacgacagccagtgacgtctggatgttcgccgtgtgcatgtg
ggagatcctgagctttgggaagcagcccttcttctggctggagaacaagg
atgtcatcggggtgctggagaaaggagaccggctgcccaagcctgatctc
tgtccaccggtcctttataccctcatgacccgctgctgggactacgaccc
cagtgaccggccccgcttcaccgagctggtgtgcagcctcagtgacgttt
atcagatggagaaggacattgccatggagcaagagaggaatgctcgctac
cgaacccccaaaatcttggagcccacagccttccaggaacccccacccaa
gccgagccgacctaagtacagaccccctccgcaaaccaacctcctggctc
caaagctgcagttccaggttcctgagggtctgtgtgccagctctcctacg
ctcaccagccctatggagtatccatctcccgttaactcactgcacacccc
acctctccaccggcacaatgtcttcaaacgccacagcatgcgggaggagg
acttcatccaacccagcagccgagaagaggcccagcagctgtgggaggct
gaaaaggtcaaaatgcggcaaatcctggacaaacagcagaagcagatggt
ggaggactaccagtggctcaggcaggaggagaagtccctggaccccatgg
tttatatgaatgataagtccccattgacgccagagaaggaggtcggctac
ctggagttcacagggcccccacagaagcccccgaggctgggcgcacagtc
catccagcccacagctaacctggaccggaccgatgacctggtgtacctca
atgtcatggagctggtgcgggccgtgctggagctcaagaatgagctctgt
cagctgccccccgagggctacgtggtggtggtgaagaatgtggggctgac
cctgcggaagctcatcgggagcgtggatgatctcctgccttccttgccgt
catcttcacggacagagatcgagggcacccagaaactgctcaacaaagac
ctggcagagctcatcaacaagatgcggctggcgcagcagaacgccgtgac
ctccctgagtgaggagtgcaagaggcagatgctgacggcttcacacaccc
tggctgtggacgccaagaacctgctcgacgctgtggaccaggccaaggtt
ctggccaatctggcccacccacctgcagagtgatctaga

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIREIITSILLSGRIGPNIRLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDAKPTCLAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDGYCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELYPYGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag. Compared to reference 2 silent changes at A960 (a2880g) and L968 (a2904g).
• Low Temp: No