cDNA Clone RSK2 (mouse)

• Synonyms: RPS6KA3, MAPKAP-K1b
• Plasmid: pCMV5-HA (mouse) RSK2
• Parent Plasmid: pCMV5 HA1
• Genbank: NM_148945.1 GI:22507356
• Tag: HA
• Species: Mouse

Sequence of Insert

ggatccatgccgctggcgcagctggcggacccgtggcagaagatggctgt
ggagagcccttccgacagcgcggagaatggacagcaaattatggatgaac
ctatgggagaggaggagattaacccacaaactgaagaaggcagtatcaaa
gaaattgcaatcacacatcatgtgaaggaaggacatgaaaaggcagatcc
ttcccagtttgaacttttaaaagtattagggcagggatcatttggaaagg
ttttcttagttaaaaaaatctcaggctctgatgctagacagctttatgcc
atgaaagtattaaagaaggccacgctgaaagttcgagaccgtgttcggac
aaaaatggaacgtgatatcttggtagaagtcaatcaccctttcattgtca
aattgcattacgcttttcaaacggaagggaagttgtatcttattttggat
tttctcaggggcggagacttgtttacacgcttatccaaagaggtgatgtt
cacagaggaagatgtcaaattctacttggctgaacttgcacttgctttag
accatcttcatagcctgggaataatctatagagacttaaaaccagaaaac
atacttcttgatgaagaaggtcacatcaagttaactgattttggcttaag
taaggaatctattgatcatgagaagaaggcttattctttttgtggcactg
tggaatacatggctccagaagtagttaaccgcagaggtcacactcagagt
gcggactggtggtcctttggtgtgttgatgtttgaaatgctcactggtac
actacctttccaaggaaaagatcgtaaagaaacaatgactatgattctta
aagccaaactcgggatgccacagtttctgagtcctgaagcccagagtctt
ttacgaatgcttttcaaacggaatcctgcaaacagattaggtgctggacc
agatggagttgaagaaattaaaagacattcatttttctcaacaatagact
ggaataaactatatagaagagagattcacccaccttttaagcctgcaact
ggcagacctgaagatacattttattttgatcctgagtttactgcaaaaac
tcccaaagattcaccgggcattccacctagtgctaacgcacatcagcttt
ttcgggggtttagttttgttgctattacctcagatgatgaaagccaagct
atgcagacagttggtgtgcattcaattgttcagcaattacacagaaacag
tattcagtttactgatggatatgaagtaaaagaggatattggcgttggct
catactccgtttgtaagagatgtatacataaagctacaaacatggagttt
gccgtgaagattattgataaaagcaagagagacccaacagaagagattga
aattcttcttcgctatggacagcatccaaacatcattaccctaaaggatg
tgtatgatgatggaaaatatgtgtatgtagtaacagaacttatgaaagga
ggtgaattgctggataagattcttagacagaagtttttctcagagcgaga
ggccagcgctgtcctgtttactataaccaaaactgttgagtatcttcatg
cacaaggggtggttcacagagacttgaaacctagcaacattctttatgtg
gatgagtctggtaatccagaatctattcgaatttgtgattttggctttgc
aaaacaactgagagcagaaaatggtcttctcatgactccttgttatactg
caaattttgttgcaccagaggttttaaaacggcaaggttatgatgctgcc
tgtgatatatggagtcttggcgtcctcctttatacaatgcttactggtta
cactccatttgcaaatggccctgatgatactccagaggaaatactggcac
gaataggtagtggaaaattctcactcagtggtggttactggaattctgtt
tcagacacagcaaaggacctggtgtcaaagatgcttcatgtagatcctca
tcagagactgacggctgctctggtgctcagacatccttggattgtccact
gggaccaactaccacaataccaactaaacagacaggatgcgccgcatctc
gtaaagggtgccatggcagctacgtactctgctttaaaccgcaatcagtc
cccagtcttggaaccagtgggccgctccactcttgctcagcggagaggga
ttaaaaaaatcacctcaacagccctgtgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEI
NPQTEEGSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKI
SGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQ
TEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLG
IIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPE
VVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMP
QFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRR
EIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFV
AITSDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKR
CIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKY
VYVVTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHR
DLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPE
VLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKF
SLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQY
QLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITST
AL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16125
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 85050 Daltons