cDNA Clone Rad5 (yeast)

• Plasmid: pRS405 Pro-13Myc-Rad5 nt1-1485 wt
• Parent Plasmid: pRS405
• Tag: 13Myc
• Species: Yeast

Sequence of Insert

AAGCTTGTTCTAGATGCCTTTGAATTTGAAAAGCATGATGATCCCTTAAG
GGATAAATGGAACACTTTACAGTTTTTGGAAAAGAGCTTTGAATCTAAAT
TTGAATCTGCATCAGAGTTAATCCAAGGAGGCGAGCTAGCTGCTATTAAA
GAAAGAAATTTTCAATTAGCCAAACTAAATAATCTTTGCTTTCGGGTACG
AGAGTCCATCAAAAGAAGACAAGACCTGGAGAAAAAGTTAAGAACTTTAT
CGCAGGACACTGACAACGAATTGCTTTTTCTGATGTTGGAAAATGAGAGA
AGAAAGAAAAGTTCAGTGATAATAGAGTTTCTTTCTGAGATAATCAGAGA
GAAATCCAAAAGATTAACTGCAGAAGAGCAAGGCTTTGTAAATCAAAATG
AAGTAAAACCCCTCATTCTGGACCTTTCTGCACGGATAAATAGATTGAAT
TCTATTCTAGAAACAAAAAATACTTGTATTAGAAGATTAAGTAATCAATA
AGTAGTTAAATTTTCATAAATTATTACTTTCTTACATGTTAGTTGGCGCG
TCGTCTGGCTGAAAATTTTACGCGTCATAAACCCTTAACTTTTCCACACA
TTATTCCTTACTGCTAAGCGCATTGCTCACTTGAAAGTAAATTATCTACA
AAGTTACATTATCAAAAGGCCTTAGAAACACACCTAAAGTCTTACAGTAT
CACAATGGCGCGCCATGGCAGGTCGACGGATCCCCGGGTTAATTAACGGT
GAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAAT
CTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTG
AAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAAC
GGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGA
ACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCT
CCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTG
AACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGG
TGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAA
TCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCT
GAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAA
CGGTGGCGCGCCTATGAGTCATATTGAACAGGAAGAAAGGAAGAGGTTTT
TTAACGATGACCTTGACACTTCAGAAACATCGTTAAACTTCAAATCTGAG
AATAAAGAGTCGTTTTTATTTGCAAATAGTCATAATGATGATGATGATGA
TGTTGTTGTATCAGTGAGTGATACAACAGAAGGAGAGGGAGACCGATCTA
TTGTGCCAGTCAGGCGAGAAATAGAAGAAGAAGGCCAGAATCAGTTTATT
ACAGAGCTTTTAAGGATTATTCCAGAAATGCCAAAGGATCTTGTGATGGA
ACTTAATGAAAAATTCGGCAGTCAGGAGGAAGGGCTCTCTTTAGCATTAT
CACATTACTTTGATCACAATAGCGGGACGTCTATCAGCAAAATACCGTCT
TCCCCGAATCAACTAAATACACTCTCAGATACCTCAAATTCAACCTTGTC
CCCATCTTCATTCCATCCTAAAAGAAGAAGAATATACGGGTTCAGAAACC
AAACACGATTAGAAGATAAAGTTACTTGGAAAAGATTTATAGGTGCTTTG
CAAGTCACTGGTATGGCTACCAGACCCACCGTCAGGCCCTTGAAGTACGG
CTCTCAGATGAAGCTAAAGAGATCAAGTGAAGAGATTTCTGCTACTAAAG
TATATGACTCACGTGGTAGAAAGAAAGCGTCCATGGCTAGTTTGGTAAGA
ATTTTTGATATCCAATATGATAGAGAAATTGGCAGAGTTTCGGAAGACAT
TGCTCAAATACTATACCCTCTTTTAAGTTCACACGAAATAAGTTTTGAAG
TTACATTGATTTTTTGTGATAATAAACGGTTGAGTATAGGTGATAGCTTT
ATTCTACAATTGGATTGCTTTTTAACATCTCTCATTTTTGAGGAACGTAA
TGATGGAGAATCCTTGATGAAAAGAAGACGTACAGAGGGAGGAAATAAAA
GAGAGAAAGACAATGGAAATTTTGGAAGAACATTGACTGAAACTGATGAA
GAGCTAGAAAGCCGCTCGAAAAGACTGGCTCTACTAAAGTTATTTGATAA
ATTGAGGCTAAAGCCTATTTTGGACGAGCAGAAGGCATTAGAAAAGCATA
AAATAGAGCTTAATAGTGACCCCGAAATCATTGATTTAGATAACGACGAG
ATTTGCTCTAATCAAGTGACTGAAGTCCATAACAATCTCCGAGATACTCA
GCACGAAGAAGAAACAATGAACTTGAATCAATTGAAAACATTTTATAAGG
CCGCACAATCATCAGAATCTTTAAAAAGTTTGCCTGAAACAGAACCTTCT
CGCGATGTCTTCAAGCTAGAACTAAGAAATTATCAAAAGCAAGGTCTTAC
TTGGATGCTAAGGAGGGAGCAAGAGTTTGCCAAAGCAGCCTCTGATGGTG
AGGCTTCAGAAACGGGTGCTAATATGATAAACCCATTATGGAAGCAGTTC
AAATGGCCAAATGATATGTCGTGGGCAGCTCAAAATTTGCAGCAGGACGC
GGCCGC

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLN
GEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
LNGLDGEQKLISEEDLNGEQKLISEEDLNGGAPMSHIEQEERKRFFNDDL
DTSETSLNFKSENKESFLFANSHNDDDDDVVVSVSDTTEGEGDRSIVPVR
REIEEEGQNQFITELLRIIPEMPKDLVMELNEKFGSQEEGLSLALSHYFD
HNSGTSISKIPSSPNQLNTLSDTSNSTLSPSSFHPKRRRIYGFRNQTRLE
DKVTWKRFIGALQVTGMATRPTVRPLKYGSQMKLKRSSEEISATKVYDSR
GRKKASMASLVRIFDIQYDREIGRVSEDIAQILYPLLSSHEISFEVTLIF
CDNKRLSIGDSFILQLDCFLTSLIFEERNDGESLMKRRRTEGGNKREKDN
GNFGRTLTETDEELESRSKRLALLKLFDKLRLKPILDEQKALEKHKIELN
SDPEIIDLDNDEICSNQVTEVHNNLRDTQHEEETMNLNQLKTFYKAAQSS
ESLKSLPETEPSRDVFKLELRNYQKQGLTWMLRREQEFAKAASDGEASET
GANMINPLWKQFKWPNDMSWAAQNLQQDAA…..

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU16093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression construct for integration.