cDNA Clone SASH1

• Plasmid: pCMV5-HA SASH1 Q715-M1247(end)
• Parent Plasmid: pCMV5 HA2
• Expressed: HA SASH1 Q715-M1247(end)
• Genbank: NM_015278.3 GI:45935384*
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCCTTGTGCGGCCGCTACATGGCCTcagggcctgagtggatgc
tcaccccgagactcaggatgctacgaaagcagtgagaacctggaaaacgg
caagactcggaaagctagcctcctatctgccaagtcatccaccgagccca
gcttgaagtcttttagcagaaaccagttgggcaattacccaacattgcct
ttaatgaaatcaggggatgcactgaagcagggacaggaggagggcaggct
gggtggtggccttgccccagacacgtccaagagctgtgacccacctggtg
tgactggtttgaataaaaaccgaagaagcctcccagtttccatctgccgg
agctgtgagaccctggagggcccccagactgtggacacttggccccgatc
ccattccctggatgaccttcaagtggagcctggtgctgagcaagacgtgc
ctaccgaggtgacagaaccgccccctcagattgtacctgaagtgccacag
aagacgaccgcctcttccacgaaggcccagcccctggagcGagactctgc
tgtcgacaatgcattgctactgacccaaagcaagagattttctgaacctc
agaaattgacaactaagaaactggagggctcaatcgcagcctctggtcgc
ggcctgtcaccccctcagtgtttgcccagaaactatgatgctcagcctcc
tggagctaaacacggtttagcaaggacgcctctggagggccacagaaaag
gacacgagtttgaaggaacacaccatcccctgggcaccaaagaaggggta
gatgctgagcagagaatgcagcccaaaattccatcacagcctccacctgt
tcctgccaaaaagagcagagaacgccttgctaacggactccaccctgttc
ccatgggccccagtggggccctccccagtcccgatgcgccatgcctgcca
gtgaaaaggggcagccccgccagccccaccagccctagcgactgtccccc
agcactggctcccaggcctctctcagggcaggcgcctggcagcccaccaa
gcacaaggccgcccccctggctctcagagctccccgagaacacaagcctc
caggagcacggtgtgaagctgggcccggctttgaccaggaaggtctcctg
tgcccggggagtggatctagaaacgctcactgaaaacaagctgcacgctg
aaggcatcgatctcacggaggagccgtattctgataagcatggccgctgt
gggattcctgaagccctggtgcagagatacgcagaggacttggatcagcc
cgagcgggacgtcgccgccaacatggaccagatccgggtgaagcagcttc
ggaagcagcaccgcatggcgattccaagtggtggactcacggaaatctgc
cgaaagcccgtctctcctgggtgcatttcgtctgtgtcagattggctcat
ttccatcggtctgcccatgtacgccggcaccctctccaccgcgggcttca
gcacactgagccaagtgccttctctgtctcacacttgccttcaggaggcc
ggcatcacagaggagagacacataagaaagctcctatctgcagccagact
cttcaaactgccgccaggccctgaggccatgtagcggccgc

Amino Acid Sequence

MYPYDVPDYAGIPGIRPCAAATWPQGLSGCSPRDSGCYESSENLENGKTR
KASLLSAKSSTEPSLKSFSRNQLGNYPTLPLMKSGDALKQGQEEGRLGGG
LAPDTSKSCDPPGVTGLNKNRRSLPVSICRSCETLEGPQTVDTWPRSHSL
DDLQVEPGAEQDVPTEVTEPPPQIVPEVPQKTTASSTKAQPLERDSAVDN
ALLLTQSKRFSEPQKLTTKKLEGSIAASGRGLSPPQCLPRNYDAQPPGAK
HGLARTPLEGHRKGHEFEGTHHPLGTKEGVDAEQRMQPKIPSQPPPVPAK
KSRERLANGLHPVPMGPSGALPSPDAPCLPVKRGSPASPTSPSDCPPALA
PRPLSGQAPGSPPSTRPPPWLSELPENTSLQEHGVKLGPALTRKVSCARG
VDLETLTENKLHAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLDQPERD
VAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGCISSVSDWLISIG
LPMYAGTLSTAGFSTLSQVPSLSHTCLQEAGITEERHIRKLLSAARLFKL
PPGPEAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11633
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015278.3 CDS:; a492g (sil), a632t (K211M) (Neither applicable in this clone) ; a2651g (Q884R); Coding diffs present in other published seqs (match AAH63279.1); ; Estimated MW of expressed protein : 59557 Daltons