cDNA Clone UBA6

• Plasmid: pCMV5-HA2 UBA6
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-UBA6
• Genbank: NM_018227.5
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGA
GGCGTCCTGTTCTTCCTGGGGGACTGGCAGCACAAATAAAAATTTGCCCA
TTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGACAG
AGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGT
TTTCTTAAGTGGGATGGGTGGTCTTGGTTTGGAAATTGCAAAGAATCTTG
TTCTTGCAGGGATTAAGGCAGTTACAATTCATGATACAGAAAAATGCCAA
GCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAA
TAAGAGAAACAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATC
CATACGTTCATGTCACATCATCTTCTGTTCCTTTCAATGAGACCACAGAT
CTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAGATGAAACT
TCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAA
TTAAGTTTATCAGTGCAGATGTACATGGAATTTGGTCAAGGTTATTTTGT
GATTTCGGTGATGAATTTGAAGTTTTAGATACAACAGGAGAAGAACCAAA
AGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACTT
GCCTTGAAAATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTT
CGAGAAATTAATGGAATGACAGGTTTAAATGGATCTATACAACAAATAAC
GGTGATATCGCCATTTTCTTTTAGTATTGGTGACACCACAGAACTGGAAC
CATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTT
TTTTTTGAATCACTGGAGAGGCAGTTAAAACATCCAAAGTGCCTTATTGT
GGATTTTAGCAACCCTGAGGCACCTTTAGAGATTCACACAGCTATGCTTG
CCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTTGGATGC
CAACAAGATTCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAAC
CTTGGAAGAGAAGCCTGATGTAAATGCTGACATTGTGCATTGGCTCTCTT
GGACTGCCCAAGGCTTTTTATCTCCACTTGCTGCAGCAGTAGGAGGTGTT
GCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTG
CCAGTGGTTATATCTTGAAGCAGCAGATATTGTTGAATCACTAGGCAAAC
CTGAATGTGAAGAATTTCTCCCACGAGGAGATAGATATGATGCCTTAAGA
GCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAATTTAAACATCTT
CTTAGTAGGGTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTT
TACTTGGTGTTGGCACAAGCAAAGAGAAAGGAATGATTACAGTTACAGAT
CCTGACTTGATAGAGAAATCCAACTTAAATAGACAGTTCCTATTTCGTCC
TCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACTCTGA
AAATAAATTCTCAAATAAAGATAGATGCACACCTGAACAAAGTATGTCCA
ACCACTGAGACCATTTACAATGATGAGTTCTATACTAAACAAGATGTAAT
TATTACAGCATTAGATAATGTGGAAGCCAGGAGATACGTAGACAGTCGTT
GCTTAGCAAATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAG
GGACACACTGAAGTTATTGTACCGCATTTGACTGAGTCTTACAATAGTCA
TCGGGATCCCCCAGAAGAGGAAATACCATTTTGTACTCTAAAATCCTTTC
CAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAGTTTGAAAGT
TCCTTTTCCCACAAACCTTCATTGTTTAACAAATTTTGGCAAACCTATTC
ATCTGCAGAAGAAGTCTTACAGAAGATACAGAGTGGACACAGTTTAGAAG
GCTGTTTTCAAGTTATAAAGTTACTTAGCAGAAGACCTAGAAATTGGTCC
CAGTGTGTAGAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCATAA
GGCTCTTCAGCTTCTTCACTGTTTCCCTCTGGACATACGATTAAAAGATG
GCAGTTTATTTTGGCAGTCACCAAAGAGGCCACCCTCTCCAATAAAATTT
GATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAGAATGCTGCAAAACT
ATATGCTACAGTATATTGTATTCCATTTGCAGAAGAGGACTTATCAGCAG
ATGCCCTCTTGAATATTCTTTCAGAAGTAAAGATTCAGGAATTCAAGCCT
TCCAATAAGGTTGTTCAAACAGATGAAACTGCAAGGAAACCAGACCATGT
TCCTATTAGCAGTGAAGATGAGAGGAATGCAATTTTCCAACTAGAAAAGG
CTATTTTATCTAATGAAGCCACCAAAAGTGACCTTCAGATGGCAGTGCTT
TCATTTGAAAAAGATGATGATCATAATGGACACATAGATTTCATCACAGC
TGCATCAAATCTTCGTGCCAAAATGTACAGCATTGAACCAGCTGACCGTT
TCAAAACAAAGCGCATAGCTGGTAAAATTATACCTGCTATAGCAACAACC
ACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATCAAAGTAACTGG
TGGCTATCCATTTGAAGCTTACAAAAATTGTTTTCTTAACTTAGCCATTC
CAATTGTAGTATTTACAGAGACAACTGAAGTAAGGAAAACTAAAATCAGA
AATGGAATATCATTTACAATTTGGGATCGATGGACCGTACATGGAAAAGA
AGATTTCACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGAA
TTGAGCCAACAATGGTGGTACAGGGAGTCAAAATGCTTTATGTTCCTGTA
ATGCCTGGTCATGCAAAAAGATTGAAGTTAACAATGCATAAACTTGTAAA
ACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTCATTTGCTCCAG
ACATTGATGGAGATGAAGATTTGCCGGGACCTCCAGTAAGATACTACTTC
AGTCATGACACTGATTAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMEGSEPVAAHQGEEASCSSWGTGSTNK
NLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIA
KNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIA
ELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQ
CPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPG
IVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTT
ELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHT
AMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVH
WLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVES
LGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLK
NFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAAD
ATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYV
DSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTL
KSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGH
SLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIR
LKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEED
LSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQ
LEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEP
ADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLN
LAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKE
KYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVS
FAPDIDGDEDLPGPPVRYYFSHDTD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11581
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 120330 Daltons