cDNA Clone Rad1 (yeast)

• Plasmid: pETDuet 6His.Rad10/Rad1
• Parent Plasmid: pETDuet1
• Genbank: DQ331482 (Rad10) M15435.1 GI:172324 (Rad1)
• Tag: 6His
• Species: Yeast

Sequence of Insert

DNA sequence of Rad10 insert :
GGATCCGATGAACAATACTGATCCTACTTCATTTGAAAGTATATTGGCTGGTGTGGCCAAATTGAGAAAGGAAAAGAGTGGTGCAGATACTACTGGATCTCAATCGTTAGAAATAGATGCATCAAAACTCCAGCAGCAGGAACCGCAAACATCAAGACGCATAAATTCAAACCAGGTCATAAACGCCTTCAATCAGCAAAAACCGGAAGAATGGACCGACTCAAAGGCTACAGATGACTATAATCGAAAGAGACCCTTCAGGAGTACTCGACCGGGAAAAACTGTACTAGTGAACACTACCCAAAAAGAAAATCCACTCCTGAACCACTTAAAGAGCACCAATTGGAGATATGTATC
TTCAACAGGAATTAATATGATATACTACGACTATCTAGTTCGTGGAAGAA
GTGTACTGTTCTTAACTTTGACTTATCACAAATTATATGTCGATTATATC
TCTAGGAGAATGCAGCCCTTATCTAGAAACGAAAATAATATACTGATATT
CATAGTAGACGATAACAACTCTGAAGATACCCTTAATGACATTACAAAAC
TATGTATGTTCAACGGATTTACTCTACTTTTAGCATTTAATTTTGAACAA
GCTGCAAAATATATTGAATATTTGAATTTATGAGGATCC


DNA sequence of Rad1 insert :
catATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGA
GGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAA
ATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAA
GATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAA
TGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGC
CAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTA
GAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACT
AGGATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAA
CATCCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCA
AAACCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTG
GTTCTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATG
ATGAACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGC
ATTGAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTAC
TTCTAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATA
GGGTTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCG
AATGAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTT
TATTAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCAC
CCCTCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGG
CCGAGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGAC
GTCACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGT
CTCAGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAG
TTAAGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAA
TGTCCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGA
ACTGGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGA
TTCCTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTT
TTTTGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCC
GAAAATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTC
ATATCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGA
AGAAAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCAC
ATGAGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCC
GACAACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAA
AAAAAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAA
AACAGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGAC
ACTTTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGA
ACAAGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGG
CCGTTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTT
TTTGAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGA
TTTGGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTG
AAATACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAA
TTTGACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCAC
AACCCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAAT
ATGTTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAAT
GACAATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGA
ACCAGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGA
AGGATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAA
GAGCAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAA
GTTGATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAG
ATCTTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAA
TTACGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAA
TCTTACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCAT
TACCAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTG
ACAGTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATC
GATTTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAAT
GTAAAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTT
GATGAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTA
TAAGAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCC
AGGATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACT
TTAAAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCT
AGAGTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATAT
TGGGAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTG
AAGGATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGG
AGTGTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCA
ATAAGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGAC
GAAGATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGA
ACAAGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCA
CTGATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCT
GTGTAActcgag

Amino Acid Sequence

Amino acid sequence of expressed 6His-Rad10 protein :
MGSSHHHHHHSQDPMNNTDPTSFESILAGVAKLRKEKSGADTTGSQSLEI
DASKLQQQEPQTSRRINSNQVINAFNQQKPEEWTDSKATDDYNRKRPFRS
TRPGKTVLVNTTQKENPLLNHLKSTNWRYVSSTGINMIYYDYLVRGRSVL
FLTLTYHKLYVDYISRRMQPLSRNENNILIFIVDDNNSEDTLNDITKLCM
FNGFTLLLAFNFEQAAKYIEYLNL*

Amino acid sequence of expressed Rad1 protein :
MSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDSNHLNEVENED
SKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTLPLPFQQKVVE
NSLITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLKRALVLVLNAK
PIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELFERPFNVVTADSLSI
EKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTGMLVLNADSLRHNSN
ESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRTKMKELRLKNVLLWP
RFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQFGLMECLKKCIAEL
SRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYESKQLVKDIRF
LRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPWLLVDEAQLVI
SYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHLQGPTLVACSD
NLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQREETKKLVKEVQSQDT
FPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEKLRNAGTNVDMEVVF
EDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQEQENEILIDDGDAEF
DNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDRQDEILISTFKSLND
NCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVYFMYYGESIEE
QSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNLAERKLKLSKL
RKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRYGIRVIPCMLT
VGDYVITPDICLERKSISDLIGSLQNNRLANQCKKMLKYYAYPTLLIEFD
EGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEIQLKLAKLVLRFPTL
KIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTNKVRSDFNSTAKGLK
DGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQKLSWNEINELINDE
DLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNALHDHHNDVPEAPV
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dual expression vector for co-expression in bacteria of 6His-tagged Rad10 and untagged Rad1.; ; All Rad1 plasmids MUST be grown at 30 degrees C or less to prevent recombination.