cDNA Clone Rad54 (yeast)

• Plasmid: pRS413 Pro-15HA-RAD54
• Parent Plasmid: pRS413
• Tag: 15HA
• Species: Yeast

Sequence of Insert

ACTAGTTAATGGTGCGTGGTTCCAGCTTCATGTGCTTGCATGTGATGTCCTGCAGATGGTAAGAAGATTCTGAAAGCCGCGCTAGGAGAAAAATATTCTGCTCGAAGATCTGTCCTCTTAAGTAGAAAGCGTGAAATTGTTGCGTTCTTGCATTACTACTCAACGCGTACGCAAATGCGTCTACTGCACCTGCATGATAAAGCTTATGTATCAAAAATTTAACATCTTGAAAATACACAAGTGGTGCAAAGATGTGTCACGTTCTGGACCTGAGTGGTGCCATGTATGCTATTTAACATGCAAAGG
GGAAGACCCTTCCGCCTTACTGCAATAATAAAAAGTATTTTACGCGTTAC
CCAATATAGCAAAGTTTCGCGCAAAAAAAAAAATAAAAAACAATTACAAA
CAAAAAGAAAAAAAAGGAAATAATAGAAGATCTAACTGAAGCGAAGGCCA
AAACTCTTCTCACTTGACGTAATAGCCGATACAAAATCTAGAGCAGCAAC
TTTTCTCTTTCTTCACTAAAGCTGCTACGAAAGTATAGAAAAATCAAACG
CTCAGAACTTAGCTCTATTTCAAGGTACCATATATATTTCCTTATAACCCATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCCATGG
CAAGACGCAGATTACCAGACAGACCACCAAATGGAATAGGAGCCGGTGAACGGCCGAGACTGGTACCTAGGCCTATTAACGTACAAGACTCGGTGAACCGACTAACGAAACCGTTCAGGGTCCCGTACAAGAACACGCACATCCCGCCCGCTGCTGGTAGAATCGCCACCGGGTCTGATAATATCGTAGGAGGAAGGAGCTTGAGGAAAAGATCAGCGACTGTATGTTATTCCGGCTTGGATATAAATGCGGACGAAGCAGAGTACAACAGCCAAGACATAAGTTTTTCTCAGTTGACTAAACGACGGAAGGATGCTCTTAGTGCTCAAAGGTTGGCCAAGGATCCGACAAGACTGAGTCATATCCAGTACACTTTGAGAAGATCTTTCACTGTGCCAATCAAGGGATATGTACAAAGACACAGTCTGCCATTAACGTTAGGAATGAAAAAAAAAATTACTCCAGAACCTCGACCTCTACATGACCCTACAGACGAATTTGCCATAGTGCTTTATGACCCGTCCGTTGACGGTGAAATGATTGTCCATGACACATCTATGGATAACAAGGAAGAAGAATCAAAGAAAATGATCAAAAGCACACAGGAAAAGGATAATATTAACAAGGAAAAGAATAGCCAGGAAGAAAGACCCACACAAAGAATAGGACGCCATCCAGCTTTGATGACAAATGGTGTGAGAAACAAACCGTTACGTGAGCTACTCGGTGATTCGGAAAACTCTGCGGAAAACAAGAAGAAATTTGCTAGTGTCCCTGTGGTTATTGATCCAAAACTGGCCAAGATTTTAAGACCCCATCAAGTCGAAGGGGTGAGATTCTTATACCGTTGTGTCACAGGGCTTGTCATGAAAGATTACTTAGAAGCTGAGGCGTTCAACACTTCAAGCGAAGATCCCTTAAAGAGCGACGAGAAGGCACTTACTGAATCTCAAAAGACTGAACAAAACAATAGAGGGGCCTACGGCTGCATCATGGCCGATGAAATGGGGCTGGGTAAGACATTGCAGTGTATAGCACTTATGTGGACATTATTAAGACAGGGCCCGCAGGGTAAAAGACTTATCGACAAATGTATCATTGTTTGTCCATCTTCTTTAGTCAACAACTGGGCTAATGAATTGATAAAATGGTTGGGTCCCAATACTCTAACTCCGTTAGCGGTTGATGGGAAAAAATCTTCAATGGGTGGTGGTAATACAACAGTTTCGCAAGCTATTCATGCCTGGGCTCAGGCACAGGGAAGAAATATTGTTAAGCCCGTTTTAATCATATCGTATGAAACATTACGTCGAAATGTTGATCAATTGAAAAACTGCAATGTGGGTTTAATGCTGGCAGATGAAGGGCACCGTTTGAAAAACGGTGACTCTTTAACATTCACAGCTTTGGATAGCATCAGTTGTCCTAGAAGAGTTATATTATCTGGTACACCCATTCAAAACGATCTTTCCGAATATTTTGCTCTACTGAGTTTTTCGAATCCTGGCTTATTAGGTTCCAGAGCAGAATTTAGGAAAAATTTTGAGAATCCAATTTTGCGAGGGCGTGATGCTGATGCTACTGATAAAGAAATCACAAAGGGCGAGGCGCAATTACAGAAGCTATCTACCATTGTCTCCAAATTTATTATCCGTCGTACTAACGATATTTTGGCCAAATATTTGCCTTGCAAGTATGAGCACGTTATTTTCGTAAACTTGAAGCCGTTGCAGAACGAGCTCTACAATAAATTAATTAAGTCAAGAGAAGTGAAAAAAGTAGTAAAAGGTGTTGGTGGGTCTCAGCCCCTGAGGGCTATTGGTATTTTAAAGAAGCTATGTAATCACCCTAATCTCTTGAATTTTGAAGATGAATTTGACGATGAAGATGATTTGGAACTACCTGACGATTATAATATGCCGGGTTCAAAAGCCAGGGACGTACAAACAAAATATTCAGCTAAATTTTCCATTTTGGAAAGATTTCTCCATAAGATAAAGACCGAATCCGATGATAAGATTGTTTTGATCTCCAACTATACCCAAACCTTGGACCTCATTGAAAAAATGTGTAGATATAAGCACTACAGTGCTGTACGGTTGGATGGTACAATGTCAATTAACAAAAGACAAAAATTGGTGGATAGGTTCAACGATCCTGAAGGCCAAGAGTTCATCTTCCTCTTAAGTTCTAAAGCAGGTGGGTGTGGTATCAATTTGATTGGTGCAAATCGATTAATTTTGATGGATCCGGATTGGAATCCTGCTGCTGATCAACAAGCTTTGGCACGTGTTTGGAGAGACGGTCAAAAAAAGGATTGTTTCATTTACAGATTCATATCGACTG
GTACCATAGAGGAAAAAATCTTTCAAAGACAATCTATGAAAATGAGTTTG
AGTTCATGCGTGGTCGATGCGAAAGAAGACGTTGAAAGGTTGTTTAGTTC
TGACAATTTAAGACAGTTGTTTCAAAAAAATGAGAATACGATATGTGAAA
CACACGAAACATACCATTGTAAACGTTGTAATGCTCAGGGTAAACAATTA
AAGAGAGCCCCTGCAATGTTATATGGTGATGCGACAACTTGGAATCATTT
GAACCATGACGCATTGGAAAAAACAAATGATCATCTACTAAAAAACGAGC
ATCATTACAATGATATCAGTTTTGCATTTCAATATATTTCACATTGAGCGGCCGC

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMARRRLPDRPPNGIGAGERPRLVPRPINVQD
SVNRLTKPFRVPYKNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGL
DINADEAEYNSQDISFSQLTKRRKDALSAQRLAKDPTRLSHIQYTLRRSF
TVPIKGYVQRHSLPLTLGMKKKITPEPRPLHDPTDEFAIVLYDPSVDGEM
IVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRIGRHPALMT
NGVRNKPLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQVEGVRF
LYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGC
IMADEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANE
LIKWLGPNTLTPLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLI
ISYETLRRNVDQLKNCNVGLMLADEGHRLKNGDSLTFTALDSISCPRRVI
LSGTPIQNDLSEYFALLSFSNPGLLGSRAEFRKNFENPILRGRDADATDK
EITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCKYEHVIFVNLKPLQNE
LYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFEDEFDDED
DLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNY
TQTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLS
SKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFI
STGTIEEKIFQRQSMKMSLSSCVVDAKEDVERLFSSDNLRQLFQKNENTI
CETHETYHCKRCNAQGKQLKRAPAMLYGDATTWNHLNHDALEKTNDHLLK
NEHHYNDISFAFQYISH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for 15HA-tagged Rad54.