cDNA Clone TSC2

• Plasmid: pRK7 FLAG TSC2 (N.B. from Blenis lab)
• Genbank: NM_000548.3 (see note below)
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACATGGACTATAAGGACGATGATGACAAAGCCAAACCAACAAGCAA
AGATTCAGGCTTGAAGGAGAAGTTTAAGATTCTGTTGGGACTGGGAACAC
CGAGGCCAAATCCCAGGTCTGCAGAGGGTAAACAGACGGAGTTTATCATC
ACCGCGGAAATACTGAGAGAACTGAGCATGGAATGTGGCCTCAACAATCG
CATCCGGATGATAGGGCAGATTTGTGAAGTCGCAAAAACCAAGAAATTTG
AAGAGCACGCAGTGGAAGCACTCTGGAAGGCGGTCGCGGATCTGTTGCAG
CCGGAGCGGCCGCTGGAGGCCCGGCACGCGGTGCTGGCTCTGCTGAAGGC
CATCGTGCAGGGGCAGGGCGAGCGTTTGGGGGTCCTCAGAGCCCTCTTCT
TTAAGGTCATCAAGGATTACCCTTCCAACGAAGACCTTCACGAAAGGCTG
GAGGTTTTCAAGGCCCTCACAGACAATGGGAGACACATCACCTACTTGGA
GGAAGAGCTGGCTGACTTTGTCCTGCAGTGGATGGATGTTGGCTTGTCCT
CGGAATTCCTTCTGGTGCTGGTGAACTTGGTCAAATTCAATAGCTGTTAC
CTCGACGAGTACATCGCAAGGATGGTTCAGATGATCTGTCTGCTGTGCGT
CCGGACCGCGTCCTCTGTGGACATAGAGGTCTCCCTGCAGGTGCTGGACG
CCGTGGTCTGCTACAACTGCCTGCCGGCTGAGAGCCTCCCGCTGTTCATC
GTTACCCTCTGTCGCACCATCAACGTCAAGGAGCTCTGCGAGCCTTGCTG
GAAGCTGATGCGGAACCTCCTTGGCACCCACCTGGGCCACAGCGCCATCT
ACAACATGTGCCACCTCATGGAGGACAGAGCCTACATGGAGGACGCGCCC
CTGCTGAGAGGAGCCGTGTTTTTTGTGGGCATGGCTCTCTGGGGAGCCCA
CCGGCTCTATTCTCTCAGGAACTCGCCGACATCTGTGTTGCCATCATTTT
ACCAGGCCATGGCATGTCCGAACGAGGTGGTGTCCTATGAGATCGTCCTG
TCCATCACCAGGCTCATCAAGAAGTATAGGAAGGAGCTCCAGGTGGTGGC
GTGGGACATTCTGCTGAACATCATCGAACGGCTCCTTCAGCAGCTCCAGA
CCTTGGACAGCCCGGAGCTCAGGACCATCGTCCATGACCTGTTGACCACG
GTGGAGGAGCTGTGTGACCAGAACGAGTTCCACGGGTCTCAGGAGAGATA
CTTTGAACTGGTGGAGAGATGTGCGGACCAGAGGCCTGAGTCCTCCCTCC
TGAACCTGATCTCCTATAGAGCGCAGTCCATCCACCCGGCCAAGGACGGC
TGGATTCAGAACCTGCAGGCGCTGATGGAGAGATTCTTCAGGAGCGAGTC
CCGAGGCGCCGTGCGCATCAAGGTGCTGGACGTGCTGTCCTTTGTGCTGC
TCATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCAGTGGTCATC
TCGCAGCTCTCCCACATCCCCGAGGATAAAGACCACCAGGTCCGAAAGCT
GGCCACCCAGTTGCTGGTGGACCTGGCAGAGGGCTGCCACACACACCACT
TCAACAGCCTGCTGGACATCATCGAGAAGGTGATGGCCCGCTCCCTCTCC
CCACCCCCGGAGCTGGAAGAAAGGGATGTGGCCGCATACTCGGCCTCCTT
GGAGGATGTGAAGACAGCCGTCCTGGGGCTTCTGGTCATCCTTCAGACCA
AGTTGTACACCCTGCCTGCAAGCCACGCCACGCGTGTGTATGAGATGCTG
GTCAGCCACATTCAGCTCCACTACAAGCACAGCTACACCCTGCCAATCGC
GAGCAGCATCCGGCTGCAGGCCTTTGACTTCCTGTTGCTGCTGCGGGCCG
ACTCACTGCACCGCCTGGGCCTGCCCAACAAGGATGGAGTCGTGCGGTTC
AGCCCCTACTGCGTCTGCGACTACATGGAGCCAGAGAGAGGCTCTGAGAA
GAAGACCAGCGGCCCCCTTTCTCCTCCCACAGGGCCTCCTGGCCCGGCGC
CTGCAGGCCCCGCCGTGCGGCTGGGGTCCGTGCCCTACTCCCTGCTCTTC
CGCGTCCTGCTGCAGTGCTTGAAGCAGGAGTCTGACTGGAAGGTGCTGAA
GCTGGTTCTGGGCAGGCTGCCTGAGTCCCTGCGCTATAAAGTGCTCATCT
TTACTTCCCCTTGCAGTGTGGACCAGCTGTGCTCTGCTCTCTGCTCCATG
CTTTCAGGCCCAAAGACACTGGAGCGGCTCCGAGGCGCCCCAGAAGGCTT
CTCCAGAACTGACTTGCACCTGGCCGTGGTTCCAGTGCTGACAGCATTAA
TCTCTTACCATAACTACCTGGACAAAACCAAACAGCGCGAGATGGTCTAC
TGCCTGGAGCAGGGCCTCATCCACCGCTGTGCCAGCCAGTGCGTCGTGGC
CTTGTCCATCTGCAGCGTGGAGATGCCTGACATCATCATCAAGGCGCTGC
CTGTTCTGGTGGTGAAGCTCACGCACATCTCAGCCACAGCCAGCATGGCC
GTCCCACTGCTGGAGTTCCTGTCCACTCTGGCCAGGCTGCCGCACCTCTA
CAGGAACTTTGCCGCGGAGCAGTATGCCAGTGTGTTCGCCATCTCCCTGC
CGTACACCAACCCCTCCAAGTTTAATCAGTACATCGTGTGTCTGGCCCAT
CACGTCATAGCCATGTGGTTCATCAGGTGCCGCCTGCCCTTCCGGAAGGA
TTTTGTCCCTTTCATCACTAAGGGCCTGCGGTCCAATGTCCTCTTGTCTT
TTGATGACACCCCCGAGAAGGACAGCTTCAGGGCCCGGAGTACTAGTCTC
AACGAGAGACCCAAGAGTCTGAGGATAGCCAGACCCCCCAAACAAGGCTT
GAATAACTCTCCACCCGTGAAAGAATTCAAGGAGAGCTCTGCAGCCGAGG
CCTTCCGGTGCCGCAGCATCAGTGTGTCTGAACATGTGGTCCGCAGCAGG
ATACAGACGTCCCTCACCAGTGCCAGCTTGGGGTCTGCAGATGAGAACTC
CGTGGCCCAGGCTGACGATAGCCTGAAAAACCTCCACCTGGAGCTCACGG
AAACCTGTCTGGACATGATGGCTCGATACGTCTTCTCCAACTTCACGGCT
GTCCCGAAGAGGTCTCCTGTGGGCGAGTTCCTCCTAGCGGGTGGCAGGAC
CAAAACCTGGCTGGTTGGGAACAAGCTTGTCACTGTGACGACAAGCGTGG
GAACCGGGACCCGGTCGTTACTAGGCCTGGACTCGGGGGAGCTGCAGTCC
GGCCCGGAGTCGAGCTCCAGCCCCGGGGTGCATGTAAGACAGACCAAGGA
GGCGCCGGCCAAGCTGGAGTCCCAGGCTGGGCAGCAGGTGTCCCGTGGGG
CCCGGGATCGGGTCCGTTCCATGTCGGGGGGCCATGGTCTTCGAGTTGGC
GCCCTGGACGTGCCGGCCTCCCAGTTCCTGGGCAGTGCCACTTCTCCAGG
ACCACGGACTGCACCAGCCGCGAAACCTGAGAAGGCCTCAGCTGGCACCC
GGGTTCCTGTGCAGGAGAAGACGAACCTGGCGGCCTATGTGCCCCTGCTG
ACCCAGGGCTGGGCGGAGATCCTGGTCCGGAGGCCCACAGGGAACACCAG
CTGGCTGATGAGCCTGGAGAACCCGCTCAGCCCTTTCTCCTCGGACATCA
ACAACATGCCCCTGCAGGAGCTGTCTAACGCCCTCATGGCGGCTGAGCGC
TTCAAGGAGCACCGGGACACAGCCCTGTACAAGTCACTGTCGGTGCCGGC
AGCCAGCACGGCCAAACCCCCTCCTCTGCCTCGCTCCAACACAGACTCCG
CCGTGGTCATGGAGGAGGGAAGTCCGGGCGAGGTTCCTGTGCTGGTGGAG
CCCCCAGGGTTGGAGGACGTTGAGGCAGCGCTAGGCATGGACAGGCGCAC
GGATGCCTACAGCAGGTCGTCCTCAGTCTCCAGCCAGGAGGAGAAGTCGC
TCCACGCGGAGGAGCTGGTTGGCAGGGGCATCCCCATCGAGCGAGTCGTC
TCCTCGGAGGGTGGCCGGCCCTCTGTGGACCTCTCCTTCCAGCCCTCGCA
GCCCCTGAGCAAGTCCAGCTCCTCTCCCGAGCTGCAGACTCTGCAGGACA
TCCTCGGGGACCCTGGGGACAAGGCCGACGTGGGCCGGCTGAGCCCTGAG
GTTAAGGCCCGGTCACAGTCAGGGACCCTGGACGGGGAAAGTGCTGCCTG
GTCGGCCTCGGGCGAAGACAGTCGGGGCCAGCCCGAGGGTCCCTTGCCTT
CCAGCTCCCCCCGCTCGCCCAGTGGCCTCCGGCCCCGAGGTTACACCATC
TCCGACTCGGCCCCATCACGCAGGGGCAAGAGAGTAGAGAGGGACGCCTT
AAAGAGCAGAGCCACAGCCTCCAATGCAGAGAAAGTGCCAGGCATCAACC
CCAGTTTCGTGTTCCTGCAGCTCTACCATTCCCCCTTCTTTGGCGACGAG
TCAAACAAGCCAATCCTGCTGCCCAATGAGTCACAGTCCTTTGAGCGGTC
GGTGCAGCTCCTCGACCAGATCCCATCATACGACACCCACAAGATCGCCG
TCCTGTATGTTGGAGAAGGCCAGAGCAACAGCGAGCTCGCCATCCTGTCC
AATGAGCATGGCTCCTACAGGTACACGGAGTTCCTGACGGGCCTGGGCCG
GCTCATCGAGCTGAAGGACTGCCAGCCGGACAAGGTGTACCTGGGAGGCC
TGGACGTGTGTGGTGAGGACGGCCAGTTCACCTACTGCTGGCACGATGAC
ATCATGCAAGCCGTCTTCCACATCGCCACCCTGATGCCCACCAAGGACGT
GGACAAGCACCGCTGCGACAAGAAGCGCCACCTGGGCAACGACTTTGTGT
CCATTGTCTACAATGACTCCGGTGAGGACTTCAAGCTTGGCACCATCAAG
GGCCAGTTCAACTTTGTCCACGTGATCGTCACCCCGCTGGACTACGAGTG
CAACCTGGTGTCCCTGCAGTGCAGGAAAGACATGGAGGGCCTTGTGGACA
CCAGCGTGGCCAAGATCGTGTCTGACCGCAACCTGCCCTTCGTGGCCCGC
CAGATGGCCCTGCACGCAAATATGGCCTCACAGGTGCATCATAGCCGCTC
CAACCCCACCGATATCTACCCCTCCAAGTGGATTGCCCGGCTCCGCCACA
TCAAGCGGCTCCGCCAGCGGATCTGCGAGGAAGCCGCCTACTCCAACCCC
AGCCTACCTCTGGTGCACCCTCCGTCCCATAGCAAAGCCCCTGCACAGAC
TCCAGCCGAGCCCACACCTGGCTATGAGGTGGGCCAGCGGAAGCGCCTCA
TCTCCTCGGTGGAGGACTTCACCGAGTTTGTGTGATCTAGA

Amino Acid Sequence

MDYKDDDDKAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITA
EILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPE
RPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEV
FKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLD
EYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVT
LCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLL
RGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSI
TRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVE
ELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWI
QNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQ
LSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPP
PELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVS
HIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSP
YCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRV
LLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLS
GPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCL
EQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVP
LLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHV
IAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNE
RPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQ
TSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVP
KRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGP
ESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGAL
DVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQ
GWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFK
EHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPP
GLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSS
EGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVK
ARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISD
SAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESN
KPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNE
HGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIM
QAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQ
FNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQM
ALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSL
PLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11409
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_000548.3 CDS:; c1723t (L575sil), g3306a (V1102sil), delta t3815-g3883 (delta V1272-A1294. Also deleted in some published sequences e.g. AAC342101, EAW85559)