cDNA Clone RAD54 (yeast)

• Plasmid: pRS413 Pro-RAD54 ATG=Nco1
• Parent Plasmid: pRS413
• Species: Yeast

Sequence of Insert

ACTAGTTAATGGTGCGTGGTTCCAGCTTCATGTGCTTGCATGTGATGTCCTGCAGATGGTAAGAAGATTCTGAAAGCCGCGCTAGGAGAAAAATATTCTGCTCGAAGATCTGTCCTCTTAAGTAGAAAGCGTGAAATTGTTGCGTTCTTGCATTACTACTCAACGCGTACGCAAATGCGTCTACTGCACCTGCATGATAAAGCTTATGTATCAAAAATTTAACATCTTGAAAATACACAAGTGGTGCAAAGATGTGTCACGTTCTGGACCTGAGTGGTGCCATGTATGCTATTTAACATGCAAAGG
GGAAGACCCTTCCGCCTTACTGCAATAATAAAAAGTATTTTACGCGTTAC
CCAATATAGCAAAGTTTCGCGCAAAAAAAAAAATAAAAAACAATTACAAA
CAAAAAGAAAAAAAAGGAAATAATAGAAGATCTAACTGAAGCGAAGGCCA
AAACTCTTCTCACTTGACGTAATAGCCGATACAAAATCTAGAGCAGCAAC
TTTTCTCTTTCTTCACTAAAGCTGCTACGAAAGTATAGAAAAATCAAACG
CTCAGAACTTAGCTCTATTTCAAGGTACCATATATATTTCCTTATAACCC
ATGGCAAGACGCAGATTACCAGACAGACCACCAAATGGAATAGGAGCCGG
TGAACGGCCGAGACTGGTACCTAGGCCTATTAACGTACAAGACTCGGTGA
ACCGACTAACGAAACCGTTCAGGGTCCCGTACAAGAACACGCACATCCCG
CCCGCTGCTGGTAGAATCGCCACCGGGTCTGATAATATCGTAGGAGGAAG
GAGCTTGAGGAAAAGATCAGCGACTGTATGTTATTCCGGCTTGGATATAA
ATGCGGACGAAGCAGAGTACAACAGCCAAGACATAAGTTTTTCTCAGTTG
ACTAAACGACGGAAGGATGCTCTTAGTGCTCAAAGGTTGGCCAAGGATCC
GACAAGACTGAGTCATATCCAGTACACTTTGAGAAGATCTTTCACTGTGC
CAATCAAGGGATATGTACAAAGACACAGTCTGCCATTAACGTTAGGAATG
AAAAAAAAAATTACTCCAGAACCTCGACCTCTACATGACCCTACAGACGA
ATTTGCCATAGTGCTTTATGACCCGTCCGTTGACGGTGAAATGATTGTCC
ATGACACATCTATGGATAACAAGGAAGAAGAATCAAAGAAAATGATCAAA
AGCACACAGGAAAAGGATAATATTAACAAGGAAAAGAATAGCCAGGAAGA
AAGACCCACACAAAGAATAGGACGCCATCCAGCTTTGATGACAAATGGTG
TGAGAAACAAACCGTTACGTGAGCTACTCGGTGATTCGGAAAACTCTGCG
GAAAACAAGAAGAAATTTGCTAGTGTCCCTGTGGTTATTGATCCAAAACT
GGCCAAGATTTTAAGACCCCATCAAGTCGAAGGGGTGAGATTCTTATACC
GTTGTGTCACAGGGCTTGTCATGAAAGATTACTTAGAAGCTGAGGCGTTC
AACACTTCAAGCGAAGATCCCTTAAAGAGCGACGAGAAGGCACTTACTGA
ATCTCAAAAGACTGAACAAAACAATAGAGGGGCCTACGGCTGCATCATGG
CCGATGAAATGGGGCTGGGTAAGACATTGCAGTGTATAGCACTTATGTGG
ACATTATTAAGACAGGGCCCGCAGGGTAAAAGACTTATCGACAAATGTAT
CATTGTTTGTCCATCTTCTTTAGTCAACAACTGGGCTAATGAATTGATAA
AATGGTTGGGTCCCAATACTCTAACTCCGTTAGCGGTTGATGGGAAAAAA
TCTTCAATGGGTGGTGGTAATACAACAGTTTCGCAAGCTATTCATGCCTG
GGCTCAGGCACAGGGAAGAAATATTGTTAAGCCCGTTTTAATCATATCGT
ATGAAACATTACGTCGAAATGTTGATCAATTGAAAAACTGCAATGTGGGT
TTAATGCTGGCAGATGAAGGGCACCGTTTGAAAAACGGTGACTCTTTAAC
ATTCACAGCTTTGGATAGCATCAGTTGTCCTAGAAGAGTTATATTATCTG
GTACACCCATTCAAAACGATCTTTCCGAATATTTTGCTCTACTGAGTTTT
TCGAATCCTGGCTTATTAGGTTCCAGAGCAGAATTTAGGAAAAATTTTGA
GAATCCAATTTTGCGAGGGCGTGATGCTGATGCTACTGATAAAGAAATCA
CAAAGGGCGAGGCGCAATTACAGAAGCTATCTACCATTGTCTCCAAATTT
ATTATCCGTCGTACTAACGATATTTTGGCCAAATATTTGCCTTGCAAGTA
TGAGCACGTTATTTTCGTAAACTTGAAGCCGTTGCAGAACGAGCTCTACA
ATAAATTAATTAAGTCAAGAGAAGTGAAAAAAGTAGTAAAAGGTGTTGGT
GGGTCTCAGCCCCTGAGGGCTATTGGTATTTTAAAGAAGCTATGTAATCA
CCCTAATCTCTTGAATTTTGAAGATGAATTTGACGATGAAGATGATTTGG
AACTACCTGACGATTATAATATGCCGGGTTCAAAAGCCAGGGACGTACAA
ACAAAATATTCAGCTAAATTTTCCATTTTGGAAAGATTTCTCCATAAGAT
AAAGACCGAATCCGATGATAAGATTGTTTTGATCTCCAACTATACCCAAA
CCTTGGACCTCATTGAAAAAATGTGTAGATATAAGCACTACAGTGCTGTA
CGGTTGGATGGTACAATGTCAATTAACAAAAGACAAAAATTGGTGGATAG
GTTCAACGATCCTGAAGGCCAAGAGTTCATCTTCCTCTTAAGTTCTAAAG
CAGGTGGGTGTGGTATCAATTTGATTGGTGCAAATCGATTAATTTTGATG
GATCCGGATTGGAATCCTGCTGCTGATCAACAAGCTTTGGCACGTGTTTG
GAGAGACGGTCAAAAAAAGGATTGTTTCATTTACAGATTCATATCGACTG
GTACCATAGAGGAAAAAATCTTTCAAAGACAATCTATGAAAATGAGTTTG
AGTTCATGCGTGGTCGATGCGAAAGAAGACGTTGAAAGGTTGTTTAGTTC
TGACAATTTAAGACAGTTGTTTCAAAAAAATGAGAATACGATATGTGAAA
CACACGAAACATACCATTGTAAACGTTGTAATGCTCAGGGTAAACAATTA
AAGAGAGCCCCTGCAATGTTATATGGTGATGCGACAACTTGGAATCATTT
GAACCATGACGCATTGGAAAAAACAAATGATCATCTACTAAAAAACGAGC
ATCATTACAATGATATCAGTTTTGCATTTCAATATATTTCACATTGAGCGGCCGC

Amino Acid Sequence

MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPYKNTHIP
PAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQL
TKRRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGM
KKKITPEPRPLHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIK
STQEKDNINKEKNSQEERPTQRIGRHPALMTNGVRNKPLRELLGDSENSA
ENKKKFASVPVVIDPKLAKILRPHQVEGVRFLYRCVTGLVMKDYLEAEAF
NTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMADEMGLGKTLQCIALMW
TLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLTPLAVDGKK
SSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVG
LMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSF
SNPGLLGSRAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKF
IIRRTNDILAKYLPCKYEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVG
GSQPLRAIGILKKLCNHPNLLNFEDEFDDEDDLELPDDYNMPGSKARDVQ
TKYSAKFSILERFLHKIKTESDDKIVLISNYTQTLDLIEKMCRYKHYSAV
RLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILM
DPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKMSL
SSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQL
KRAPAMLYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11380
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged Rad54.