cDNA Clone slit-robo-GAP 3

• Plasmid: pGEX6P-1-srGAP3b
• Parent Plasmid: pGEX6P1
• Expressed: GST-srGAP3b
• Genbank: NM_001033117
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatgtcatctcaaactaagttcaagaaagacaaagagatcattgctgaatatgaagcccaaataaaagagatccgcacgcagctggtggagcagttcaaatgtctggagcagcaatcagagtcgcgactgcagctgcttcaagacctccaggagtttttccgccggaaagctgagattgagctcgagtactcccgcagcctggagaagctggctgagcgcttctcctccaaaatccgcagctcccgggagcaccagttcaagaaggaccagtacctcctctcgcctgtgaactgttggtat
ctggttctgcatcagacccggcgggagagccgagaccatgccaccctcaa
tgacatcttcatgaacaatgtcatcgtccgcctctcccagatcagtgagg
atgtcatcagactcttcaaaaagagcaaggagattggcctgcagatgcac
gaggagctcctgaaggtgaccaatgagctctacacagtcatgaaaaccta
ccacatgtaccatgcagagagcatcagtgcggaaagcaagctgaaggagg
ctgagaagcaggaggagaagcagttcaataagtcaggagacctcagcatg
aacctgctccggcacgaggaccggccccagcgccgcagctctgtgaagaa
gattgagaagatgaaggagaagaggcaggccaagtactctgagaacaagc
tgaaatgcacaaaggcccggaatgactacttgctcaatctggcagccacc
aacgcagctataagcaaatactacatccatgatgtctctgatctgatcga
ttgctgtgatttgggcttccatgccagcctggcccgcaccttccggacct
atctctcagctgaatacaacctggagacctctcgccacgaagggctggat
gtcattgagaatgcagtggacaacctggattcccgaagtgacaagcacac
agtcatggacatgtgcaatcaagtcttctgccctccactcaagttcgagt
tccagccccacatgggggatgaggtctgccaggtcagcgctcagcagccc
gtccagacagaactgctcatgcgttatcaccagctgcagtccagactggc
caccctcaagatagagaatgaggaggttaggaaaaccctggatgccacca
tgcagacattacaggacatgctgactgtggaggactttgatgtctccgat
gccttccaacacagtcgatcgacagagtccgtcaagtcggctgcctctga
gacctacatgagcaagatcaacattgccaagaggagagccaaccagcagg
aaacagaaatgttttattttacaaaatttaaagagtatgtgaatggcagt
aacctcatcaccaagctgcaggccaagcacgatttactcaagcagaccct
gggcgaaggggaaagagcagaatgcggcaccaccagaggaagaagaaatg
ctcgaaccaggaaccaggattcaggacaagctataccgcttgtagtcgag
agctgcatccgttacatcaatttatatggactccagcagcaggggatctt
cagagtgccaggatctcaggtggaagtcaatgacatcaaaaattcctttg
agagaggtgaagacccccttgtggacgatcaaaatgaacgagatatcaat
tcagtcgctggtgttttaaaactgtatttccgaggactggaaaacccact
ctttcctaaggaaaggtttcaagatttgatatctactattaaactggaga
acccagccgagagggtgcaccagatccaacaaatcctcgtcacccttccc
cgcgtggtcattgtggtcatgagatacctcttcgctttcctcaaccacct
ctcccagtatagcgacgagaacatgatggatccctacaacctggccatct
gcttcgggcctaccctcatgcacatccctgatgggcaggaccctgtgtcc
tgccaggcacacatcaatgaagtcatcaaaaccatcatcatccatcatga
agccatcttccccagcccccgggagctagagggacctgtgtatgaaaaat
gcatggctggaggggaagaatattgtgacagcccacacagcgagccaggg
gccatcgatgaagttgaccatgacaatggcactgagcctcataccagcga
tgaagaagtggagcagatcgaggctattgccaagtttgactacatggggc
ggtccccgcgtgagctatccttcaagaagggggcctcgctgctcctgtac
caccgcgcctcggaggactggtgggagggccggcacaacggcgtggatgg
actcatcccccatcagtacatagttgtacaggacatggatgatgccttct
ccgacagcctgagccagaaggctgacagcgaggccagcagtgggccattg
ctggatgacaaggcctcttccaaaaacgacctccagtcccccacggagca
catctcggattacggctttgggggggtgatgggccgagtgcggttacgat
ctgatggagcagccatccccagacgccgaagcgggggcgacacacacagc
ccgccccggggcctgggccccagcatagacacaccaccccgggctgctgc
ctgccccagcagcccccacaaaatccccctcacccgggggaggatcgaga
gccctgagaagcggaggatggcgacgttcgggagcgctggcagcatcaac
taccctgacaagaaggcgctctccgaagggcactcgatgaggtcgacctg
cggttccaccaggcacagcagcctaggggaccacaagtccctggaggccg
aggccctggcagaagacatcgagaagaccatgagcacggctctgcacgag
ttgcgggaactcgagaggcagaacacggtcaagcaggcgccagatgtggt
gctggacaccctggagcccctgaagaacccgccaggccccgtcagctcgg
agcccgccagtccccttcacaccatcgtcatccgcgaccccgatgccgcc
atgcgccgcagcagcagctcctccaccgagatgatgaccaccttcaagcc
agccctgtccgcccgcctggctggcgcccagctccgcccgccccccatgc
ggcccgtgcggccggtggtccagcaccggtccagcagcagcagcagctcg
ggcgtgggcagcccggccgtgacgcccaccgagaagatgttccccaacag
ctcagcggacaagtcgggcaccatgtgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSSQTKFKKDKEIIAE
YEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRS
LEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATL
NDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKT
YHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVK
KIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLI
DCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKH
TVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRL
ATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAAS
ETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQT
LGEGERAECGTTRGRRNARTRNQDSGQAIPLVVESCIRYINLYGLQQQGI
FRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENP
LFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVVMRYLFAFLNH
LSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHINEVIKTIIIHH
EAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPGAIDEVDHDNGTEPHTS
DEEVEQIEAIAKFDYMGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVD
GLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE
HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAA
ACPSSPHKIPLTRGRIESPEKRRMATFGSAGSINYPDKKALSEGHSMRST
CGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELERQNTVKQAPDV
VLDTLEPLKNPPGPVSSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFK
PALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPN
SSADKSGTM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11369
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 148973 Daltons