cDNA Clone slit-robo-GAP 2

• Plasmid: pGEX6P-2-slit-robo-GAP 2a
• Parent Plasmid: pGEX6P2
• Expressed: GST-srGAP2a
• Genbank: NM_015326
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgatgacgtctccagccaaattcaaaaaggataaggagatcatagcagagtacgatactcaggtcaaagagatccgtgctcagctcacagagcagatgaaatgcctggaccagcagtgtgagcttcgggtgcaactgttgcaggacctccaggacttcttccgaaagaaggcagagattgagatggactactcccgcaacctggagaagctggcagaacgcttcctggccaagacacgcagcaccaaggaccagcaattcaagaaggatcagaatgttctctctccagtcaactgctggaatctcctcttaaaccaggtgaagcgggaaagcagggaccataccaccctgagtgacatctacctgaataatatcattcctcgatttgtacaagtcagcgaggactcaggaagactctttaaaaagagtaaagaagtcggccagcagctccaa
gatgatttgatgaaggtcctgaacgagctctactcggtcatgaagacata
tcacatgtacaatgccgacagcatcagtgctcagagcaaactaaaggagg
cggagaagcaggaggagaagcaaattggtaaatcggtaaagcaggaggac
cggcagaccccacgctcccctgactccacggccaatgttcgcattgagga
gaaacatgtccggaggagctcagtgaagaagattgagaagatgaaggaga
agcgccaagccaagtacacggagaataagctgaaggccatcaaagcccgg
aatgagtacttgctggctttggaggcaaccaatgcatctgtcttcaagta
ctacatccatgacctatctgaccttattgatcagtgttgtgacttaggct
accatgcaagtctgaaccgggctctacgcaccttcctctctgctgagtta
aacctggaacagtcgaagcatgagggtctggatgccatcgagaatgcagt
agaaaacctggatgccaccagtgacaagcagcgcctcatggagatgtaca
acaacgtcttctgcccccctatgaagtttgagtttcagccccacatgggg
gatatggcttcccagctctgtgcccagcagcctgtccagagtgagctggt
acagagatgccaacaactgcagtctcgcttatccactctaaagattgaaa
acgaagaggtaaagaagacaatggaggccaccctgcaaaccatccaggac
attgtgactgtcgaggactttgatgtgtctgactgcttccagtacagcaa
ctccatggagtccgtcaagtccacggtctctgaaaccttcatgagcaagc
ccagcattgctaagaggagagccaaccagcaagagacagagcagttttat
ttcacaaaaatgaaagagtacctggagggcaggaacctcatcaccaagtt
acaagccaagcatgaccttctgcagaaaaccctgggagaaagtcagcgga
cagattgcagtctagccaggcgcagctcaactgtgaggaaacaggactcc
agccaggcaattcctctagtggtggaaagctgtatccggtttatcagcag
acacggactacagcatgaaggaattttccgggtgtcaggatcccaggtgg
aagtgaatgacatcaaaaatgcctttgagagaggagaggaccccctggct
ggggaccagaacgaccatgacatggattccatagctggtgtcctgaagct
ttacttccgggggctggaacaccctctcttccccaaggacatctttcatg
acctgatggcctgcgtcacaatggacaacctgcaggagagagctctgcac
atccggaaagtcctcctagtcctgcccaagaccactctgattatcatgag
atacctctttgccttcctcaatcatttatcacagttcagtgaagagaaca
tgatggacccctacaacctcgccatctgcttcgggccctcgctaatgtca
gtgccagagggccacgaccaggtgtcctgccaagcccacgtgaatgagct
gatcaaaaccatcatcatccagcatgagaacatcttcccaagccccaggg
agctggagggccctgtctacagcagaggaggaagcatggaggattactgt
gatagccctcatggagagactacctcggttgaagactcaacccaggatgt
gaccgcagagcaccacacgagcgatgacgaatgtgagcccatcgaggcca
ttgccaagtttgactacgtgggccggacagcccgagagctgtcctttaag
aagggagcatccctgctgctttaccagcgggcttccgacgactggtggga
aggccggcacaatggcatcgacggactcatcccccatcagtacatcgtgg
tccaagacaccgaggacggtgtcgtggagaggtccagccccaagtctgag
attgaggtcatttctgagccacctgaagaaaaggtgacagccagagcggg
ggccagctgtcccagtgggggtcatgtagccgatatttatcttgcaaaca
tcaacaagcaaaggaagcgtccagaatctgggagcatccggaaaactttt
cggagtgacagccacgggctgagcagttccctgactgactcctcctcccc
aggggtgggggctagctgccgcccatcctcccagcccatcatgagccaga
gcctccccaaagaagggccagataagtgttccatcagtgggcacgggagc
ctcaactccatcagccgccactcatccctgaagaatcggctggatagtcc
acagatccggaagactgccacagcgggaaggtcaaaaagcttcaataacc
atcggcccatggaccctgaggtcattgctcaggatattgaggcaacaatg
aactcggccctgaatgagctacgggaactagaacggcagagcagtgtcaa
acacacccctgacgtggttctggacaccttggagcccctcaaaacctccc
cagtggtggcccccacgtcagagccctccagccctctgcacacccagctc
ctcaaggaccccgagcccgccttccagcgcagcgccagtactgctgggga
catcgcctgcgccttccggcctgtcaagtctgtcaagatggctgccccgg
tcaaaccaccagccacacggcccaagcccactgtcttccccaaaacaaat
gccactagccctggtgtcaactcatcaacttccccacagtctactgacaa
gtcttgtactgtctgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMTSPAKFK
KDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAE
IEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKR
ESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLN
ELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPD
STANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALE
ATNASVFKYYIHDLSDLIDQCCDLGYHASLNRALRTFLSAELNLEQSKHE
GLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCA
QQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFD
VSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYL
EGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVV
ESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDM
DSIAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVL
PKTTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQV
SCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYCDSPHGETT
SVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLY
QRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVISEPP
EEKVTARAGASCPSGGHVADIYLANINKQRKRPESGSIRKTFRSDSHGLS
SSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISGHGSLNSISRHS
SLKNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELR
ELERQSSVKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAF
QRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNS
STSPQSTDKSCTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11367
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences compared with NM_015326 CDS: g489c, c636t, t705c, g1518a, a1830g, t2565c - all silent.; ; Estimated MW of expressed protein : 148741 Daltons