cDNA Clone slit-robo-GAP 1

• Plasmid: pGEX6P-2-slit-robo-GAP 1 isoform 2
• Parent Plasmid: pGEX6P2
• Expressed: GST-srGAP1 isoform 2
• Genbank: AB037725*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAACAAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGATCTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCGGAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTAAGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTTGAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTGAGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTCCAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGATGAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAGAGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGATCCAGTCTTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGTAAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAAATAAGCTAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAAGCCACCAATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTTAATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAAGAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGCTTAGACATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAAGCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGTTTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCAGTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCGCCTTGCCACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAGCCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTTTCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGTCTCTGAAACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACCAGCAGGAAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAAGGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAGGACCCTGGGAGAAGGTCATAGAGCTGAATATATGACTACAAGCCGAGGACGAAGAAACTCGCACACAAGACATCAGGACTCAGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATCTCTATGGTCTTCAGCATCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTCAATGATATTAAAAATTCATTTGAGAGAGGTGAAAATCCTTTGGCTGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGCTCTATTTCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAACGATCTGATTTCTTGTATCAGAATAGATAATCTCTATGAGAGGGCGCTTCACATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTGATGAGGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAATATGATGGACCCTTATAACCTGGCCATTTGCTTTGGCCCAACATTGATGCCTGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGCACATGTGAATGAAATTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAAGAGCTGGATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTGCGACAGCCCATACAGTGAGCACGGTACATTGGAGGAAGTGGACCAAGATGCTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGAAGCAATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAAGAAGGGTGCCTCCCTGCTGCTGTATCACCGTGCATCTGAGGACTGGTGGGAAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTCACCAGTATATAGTGGTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGACAGTGAGGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACAGACCGTCATCCTGACGGCTATTTAGCCAGGCAACGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGACCAGTGATGGCCATTGCCCGCTC
CACCCTCCACATGCCCTTTCTAACTCCTCAGTTGACCTAGGGTCCCCAAG
CCTTGCCAGTCACCCCCGGGGCCTGCTGCAGAACCGTGGCCTCAACAATG
ACAGTCCTGAGCGGAGGCGCAGGCCTGGCCATGGCAGCCTGACCAACATC
AGCCGGCACGACTCCCTCAAGAAGATCGACAGCCCTCCCATTAGAAGGTC
CACGTCATCAGGGCAATACACGGGCTTCAATGACCACAAGCCACTGGACC
CAGAGACAATTGCTCAGGATATTGAAGAAACGATGAACACAGCTTTGAAT
GAACTCCGAGAACTGGAGAGACAGAGCACAGCAAAGCATGCCCCTGATGT
GGTGCTGGATACCCTGGAGCAAGTGAAAAACTCTCCCACCCCTGCCACTT
CCACGGAATCTCTCAGCCCTTTGCACAACGTTGCCCTCAGGAGCTCCGAG
CCTCAGATTCGACGTAGCACGAGCTCCTCCAGTGACACAATGAGTACTTT
CAAGCCTATGGTGGCACCCAGAATGGGCGTGCAGCTGAAGCCTCCAGCCC
TTAGGCCAAAACCTGCTGTTCTTCCAAAAACAAATCCTACCATAGGACCT
GCCCCACCTCCCCAGGGTCCAACAGACAAGTCATGCACAATGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTPSRFKKDKEIIAEYES
QVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEK
LAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSD
IYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYH
MYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRSSVKKIE
KMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCC
DLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFM
EMYPAAFCPPMKFEFQSHMGDEVCQVSAQQPVQAELMLRYQQLQSRLATL
KIENEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETY
LSKPSIAKRRANQQETEQFYFMKLREYLEGSNLITKLQAKHDLLQRTLGE
GHRAEYMTTSRGRRNSHTRHQDSGQVIPLIVESCIRFINLYGLQHQGIFR
VSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLF
PKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFLNHLS
QYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHET
IFPDAKELDGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDE
CEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLV
PHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHPD
GYLARQRKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASH
PRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSG
QYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDT
LEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMV
APRMGVQLKPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11366
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Start codon not identified in AB037725. This clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein : 148414 Daltons