cDNA Clone RTT101 (yeast)

• Plasmid: pRS413 Pro-15HA-RTT101
• Parent Plasmid: pRS413
• Genbank: Z49322
• Tag: HA
• Species: Yeast

Sequence of Insert

ACTAGTTCCAATTGTGAAGCAGACATGATCTGTTTGGCATATCCAATATTATAGAAATTGTACAAGTCTTTATTAAAAGCACTGATATCTTCTTCCATAGAGCTGGCCTTCGTCGTATCATAATCAGATTTACCTTCATCCTGCAAAATTGAAACGTTATCATTTGAAGGTGTAAACATGTTTTCATACATTGAGTTTATATCATGATATTTATCGTCTGCGTCTTTTGCCCATCGTGTAAGTGTGTGAAATAAAGTGGTGCAATGTATGAGGTTTCGCTGTCCCACATATCGAGGGGAGAGTGCC
CAATTACTTAGCATCATAGACGTAATGTAAACATCAAAGAAAAGTCTAGT
GTTTTCTTCTTTTGTATTCATCAAAAATTCTACACACGTGAGCTCGGAGA
TTTTTGGATGTTCAATATTCCTGATTAAGTATGACGAAGCGGAACGCATA
GCTATCCTTTGCGTAATGATGAATATGAACAATGCGAATGAAGATCATCG
ATATCTTTGCACTAAAAGACTACTTTGAAGGAATCCTCAAGATATTAAAC
CCACCAATCTTTTTTACTGGTATAAATTCTCGTACGGGTTCACAGGAACCATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCCATGG
TAAATGAGAGCGTTTCCAAGAGAGAAGGATTCCACGAGTCTATAAGCAGAGAGACGAGTGCAAGCAACGCTTTAGGACTATACAATAAATTTAACGATGAGCGAAATCCCAGGTATCGAACGATGATTGCTGAGCTGCACGAGTTTTTTCACCTTACTTTAGCTGAAACTATAACTGAAACTGACGTGAAAGAATTGGAGTGTAATAAAGAAAAGGCCGCTAAATTTAGAAAGCTAATGCCTAAGATGTTGAACAATTGCCGTGAATTAACGCAAAGGAAATCATACATTCCTTACAACTCAGAATTTAATGGGAATGACGAAAAGCAAAAGAAGTTTCAGCTGCTACATCAACACCAAATTGTGCTGAGTTTTCAAGAATTTTGTGATGAATTGGCAAAGTTAATAATTGACGCGCACGTCCTTAGCTTTTTGACCAGATGCGACTATTCTTATGAAATTATACCCAAAAATTGGACCTCTTTTTACAAGCTATTTCAGTACGTAATGGGTGCCGTTGGTCCTATTATATCGTATGTGCCGGTAAATTATCCCATGATAAGAAAAGAACTAGGATTTGAGACCTTAACGATATTTCAGTATTATGATTCCAAACTTTTCGAATGTATGAAGTCACATTTTGGAAGGGAATTTTCCACCCTTGTAAGCGCTACTATTCATCACTATATTCATATGTTTCCAATAACGAATACAATGTTAGAGAAGGAAGTTCCTATGCTCCGTATAATGTCTAACTGCAATTTTAGTATAGAGGGCTTGAGCCCCAAAGATTTTTATATGAAAACTCTCAGACAATATTACTGTGAGGAATCAAATTTAAGGCCGAGGCTGGAAACTTTCAAGAATTTTAAGGTACTATTGACGAGGAATGCATTATTAGCATCTTTATTTTCCCCTGAATGGGTCAGCGATGCAAATGACCTATTTATAAGCCATCTACTGTTGAACAAGAAGTCTATTAGCGAATACATAGAAATTGGGAAGGACACTTATGATGAAGAAAAAGAGAGATATTTCAAAACTGAGACACACTTTAGCTTATTGATGTTTAGAAATGCTTTTGAAGCAAAAAATATGCTGTCAAAATTCAAGGAATTCTGTGATGATGCCGTTTCAGAAAAGTTAAAGGCAGCATATGGATCCAATCATGATACTGAAAGGCTATTTGATGAGGTAGTTCAGCTAGCTAATGTAGATCACCTCAAAATATACTCCGATTCCATCGAGTACCATTTATGTAATTTACTTGGAAGTACCAGCAAAGCCATAGAACAGTATGTCAAATATTTTGAGTCACATTTATTCATCATCGTCAGAAAGATAAAAACAACTAAAAAAGACCTACCCAGAGATATGAAAATAAAGTATCTCAACGAAAATCTTCCTATATTGAGACTGAAGTTCGTTAACCTTCCCACATTTCCTAATTTTTTCGAAAGATCAATTTTCAGAAAGACGATCTTACAAAGTGATCAAAATTCCTCATTTATTAAGGATATATTACCTGTTTACAAAGACAGCCTCATGGAACTTTTCAAACAAAGAATCATAACAAATGTCTCACAAGAGGATGAAATGCGATACCGAGATCAATATCAACCCTATTTATCGCAATTCTTTCAGCCGGTAGAGGTTATGGCAGATCTGAGAATCAAATATGCATCGTTCTTGTCGTTTTATGAGAATATCGAGGCTGCTGTGAAGTTCGGGAAGACATACAATGAAAACAACAGCAAGTCATTTTTCCCACTTATCTTTGATAGAGAAAGAATTCCCAAAGTTTTTCAGCAATCCAACGAAGTAAAGAAAAATTTTGTCCTACCTCAAGAGATGGATGATACGTGGAACCAATTTCTCCGTAATTATCACGAGCAGAATAAAGTGGAAGATTCAGATGCTTCCAAAAAAGAGCTATATCCGATGTGGAACTTGCACCACTGCGAAGTGGAGTCGCCTTATATCATACAGGATGGAACTAACCTAATATTTGAATTGACCCTTTTCCAAACATGTGTGCTGACTTTATTCAACGAAAGCGATCATTTGACCTTGCAAGTTATATCAGAGCAAACGAAACTGGCGTACAAAGACTTAGCGCTAGTCTTAAAATCCTTCTGCAATTATAAAATTCTGACAAGAGATATTGACAACACATACAGTATCAACGAGAGCTTCAAACCTGATATGAAAAAAGTGAAAAATGGGAAACTACGTGTGGTTCTACCGAGAACCGCGTCTCTCCAAAGTAGCAACACAGGCGGTGAAAGAACCAGTTCTGCACACCACGAAGGAAGTAATTCCCAATGGACCCAAGAATTACTAAAGG
CATGCATAACTAGATCCGTTAAGAGCGAGAGGAATGGGCTAGACTACGAC
CATTTATTTGAAACGGTAAAGCAGCAGATAAAAGGCTTTAGTGTTGGTGA
GTTCAAAGACGCCTTGGCCAAGTTGTTACGAGACAAATTCATAACTAGGG
ACGAATCAACAGCAACTTACAAGTACTAAGCGGCCGC

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMVNESVSKREGFHESISRETSASNALGLYNK
FNDERNPRYRTMIAELHEFFHLTLAETITETDVKELECNKEKAAKFRKLM
PKMLNNCRELTQRKSYIPYNSEFNGNDEKQKKFQLLHQHQIVLSFQEFCD
ELAKLIIDAHVLSFLTRCDYSYEIIPKNWTSFYKLFQYVMGAVGPIISYV
PVNYPMIRKELGFETLTIFQYYDSKLFECMKSHFGREFSTLVSATIHHYI
HMFPITNTMLEKEVPMLRIMSNCNFSIEGLSPKDFYMKTLRQYYCEESNL
RPRLETFKNFKVLLTRNALLASLFSPEWVSDANDLFISHLLLNKKSISEY
IEIGKDTYDEEKERYFKTETHFSLLMFRNAFEAKNMLSKFKEFCDDAVSE
KLKAAYGSNHDTERLFDEVVQLANVDHLKIYSDSIEYHLCNLLGSTSKAI
EQYVKYFESHLFIIVRKIKTTKKDLPRDMKIKYLNENLPILRLKFVNLPT
FPNFFERSIFRKTILQSDQNSSFIKDILPVYKDSLMELFKQRIITNVSQE
DEMRYRDQYQPYLSQFFQPVEVMADLRIKYASFLSFYENIEAAVKFGKTY
NENNSKSFFPLIFDRERIPKVFQQSNEVKKNFVLPQEMDDTWNQFLRNYH
EQNKVEDSDASKKELYPMWNLHHCEVESPYIIQDGTNLIFELTLFQTCVL
TLFNESDHLTLQVISEQTKLAYKDLALVLKSFCNYKILTRDIDNTYSINE
SFKPDMKKVKNGKLRVVLPRTASLQSSNTGGERTSSAHHEGSNSQWTQEL
LKACITRSVKSERNGLDYDHLFETVKQQIKGFSVGEFKDALAKLLRDKFI
TRDESTATYKY*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11360
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for 15HA-tagged RTT101.; Sequence differences compared with ref Z49322:; a322g and t385c in promoter sequence - in all other rtt101 pro constructs.; I2V of RTT101 due to conversion of ATG to Nco1 site.