cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA slit-robo-GAP1 isoform 2 C685-S842
• Parent Plasmid: pCMV5 HA1
• Expressed: HA slit-robo-GAP1 isoform 2 C685-S842
• Genbank: AB037725*
• Tag: HA
• Species: Human

Sequence of Insert

ggatcctgtatggctggagatgactattgcgacagcccatacagtgagcacggtacattggaggaagtggaccaagatgctggtacagagccccacacaagtgaagatgaatgtgagccaatagaagcaatagccaagtttgactatgttgggcggtccgccagagaactatccttcaagaagggtgcctccctgctgctgtatcaccgtgcatctgaggactggtgggaaggcaggcacaacgggattgacgggctggtgcctcaccagtatatagtggtgcaggatatggatgatacgttttca
gacactctgagccaaaaagccgacagtgaggccagcagtgggccagtcacggaagacaagtcctcatccaaggacatgaactccccgacagaccgtcatcctgacggctatttagccaggcaacgaaaaagaggagagccaccccctccagtaaggcgtcctggcaggaccagttaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEP
IEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQ
YIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYL
ARQRKRGEPPPPVRRPGRTS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11330
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. Numbering for this clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein : 18960 Daltons