cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA slit-robo-GAP1 isoform 2 G468-K684
• Parent Plasmid: pCMV5 HA1
• Expressed: HA slit-robo-GAP1 isoform 2 G468-K684
• Genbank: AB037725*
• Tag: HA
• Species: Human

Sequence of Insert

ggatccggagaaggtcatagagctgaatatatgactacaagccgaggacgaagaaactcgcacacaagacatcaggactcaggacaggttattcccctcattgtggaaagctgtattcggttcatcaatctctatggtcttcagcatcaggggattttcagagtgtctggttcccaggtggaagtcaatgatattaaaaattcatttgagagaggtgaaaatcctttggctgatgaccagagtaaccatgatattaactcagttgctggcgttctgaagctctatttccgtgggctggaaaacccc
ctctttcctaaggaaagatttaacgatctgatttcttgtatcagaataga
taatctctatgagagggcgcttcacatccgcaaactcctcctgactttgc
ccaggtcggtccttatagtgatgaggtacctctttgccttcctcaatcat
ctatcacagtacagcgatgagaatatgatggacccttataacctggccat
ttgctttggcccaacattgatgcctgtcccagaaatacaggatcaagtgt
cttgccaggcacatgtgaatgaaattatcaaaaccatcatcatccaccat
gagactattttcccagatgctaaagagctggatggccctgtttatgagaa
ataagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSGEGHRAEYMTTSRGRRNSHTRHQDSGQVIPLIVESCIR
FINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAG
VLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVL
IVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAH
VNEIIKTIIIHHETIFPDAKELDGPVYEK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11329
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. Numbering for this clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein : 26320 Daltons