cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA slit-robo-GAP1 isoform 2 M1-L467
• Parent Plasmid: pCMV5 HA1
• Expressed: HA slit-robo-GAP1 isoform 2 M1-L467
• Genbank: AB037725*
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatgtccaccccgagccgattcaagaaggacaaagagatcatagccgagtatgaaagtcaagtcaaagaaattcgagctcaactggtagaacaacaaaaatgcctggagcagcaaacggagatgcgagttcagcttctccaggatctgcaagatttcttccgaaaaaaagctgaaattgagacggaatattcccggaatctagagaagttagcagaaaggttcatggcaaaaacaagaagcactaaggatcatcaacaatacaagaaagaccagaacctgttgtctccagtgaactgctgg
tatttgctcctgaaccaagtaaggagagaaagcaaagaccatgcaacctt
gagtgacatctatctgaacaatgtgattatgcggttcatgcagataagtg
aggattctaccaggatgtttaaaaagagcaaagagattgcattccaactt
catgaggatttaatgaaggttcttaatgagctttatacggtgatgaaaac
ataccatatgtatcatgcagagagcatcagtgcagagagcaagctgaaag
aggccgaaaaacaagaggaaaagcaaattgggagatctggtgatccagtc
ttccatattcgactagaggagagacatcaacggcgaagctctgtaaagaa
aattgaaaaaatgaaagaaaaaagacaagcaaaatattcagaaaataagc
taaaatcaattaaggcacggaacgaatatctcctaacacttgaagccacc
aatgcctcagttttcaagtactatattcatgatctttctgatttaattga
ttgctgtgatcttggctaccatgcaagtctgaacagagccctaagaacat
atctgtctgcggagtacaaccttgaaacctccagacatgagggcttagac
attattgagaatgcagttgataatttagagcccaggagcgataagcagag
attcatggagatgtaccctgctgcgttctgtccaccaatgaagtttgagt
ttcagtctcacatgggtgatgaggtgtgccaggtcagtgcccagcagcca
gtccaggcagagctcatgctcaggtaccaacagttgcagtcccgccttgc
cacgctcaaaatcgagaatgaagaggttaagaaaacgactgaagccacct
tgcagacgatacaagatatggtcaccatcgaggactatgatgtttctgaa
tgcttccagcacagtcgttccacagaatctgtgaagtccactgtctctga
aacctacctgagtaaacccagcatcgccaagagaagagccaaccagcagg
aaactgaacagttctacttcatgaaactcagagaatatttggaaggcagt
aatctcatcacaaaacttcaagccaaacatgacttgctgcagaggaccct
gtaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQ
TEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYK
KDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTR
MFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQ
EEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIK
ARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAE
YNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHM
GDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQ
DMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQF
YFMKLREYLEGSNLITKLQAKHDLLQRTL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. This clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein: 56620 Daltons