cDNA Clone 14-3-3 beta

• Synonyms: YWHAB
• Plasmid: pGEX6P-14-3-3 beta
• Parent Plasmid: pGEX6P1
• Expressed: GST-14-3-3 beta
• Genbank: NM_139323.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACAATGGATAAAAGTGAGCTGGTACAGAAAGCCAAACTCGC
TGAGCAGGCTGAGCGATATGATGATATGGCTGCAGCCATGAAGGCAGTCA
CAGAACAGGGGCATGAACTCTCCAACGAAGAGAGAAATCTGCTCTCTGTT
GCCTACAAGAATGTGGTAGGCGCCCGCCGCTCTTCCTGGCGTGTCATCTC
CAGCATTGAGCAGAAAACAGAGAGGAATGAGAAGAAGCAGCAGATGGGCA
AAGAGTACCGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGAT
GTTCTGGAGCTGTTGGACAAATATCTTATTCCCAATGCTACACAACCAGA
AAGTAAGGTGTTCTACTTGAAAATGAAAGGAGATTATTTTAGGTATCTTT
CTGAAGTGGCATCTGGAGACAACAAACAAACCACTGTGTCGAACTCCCAG
CAGGCTTACCAGGAAGCATTTGAAATTAGTAAGAAAGAAATGCAGCCTAC
ACACCCAATTCGTCTTGGTCTGGCACTAAATTTCTCAGTCTTTTACTATG
AGATTCTAAACTCTCCTGAAAAGGCCTGTAGCCTGGCAAAAACGGCATTT
GATGAAGCAATTGCTGAATTGGATACGCTGAATGAAGAGTCTTATAAAGA
CAGCACTCTGATCATGCAGTTACTTAGGGACAATCTCACTCTGTGGACAT
CGGAAAACCAGGGAGACGAAGGAGACGCTGGGGAGGGAGAGAACTAAGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTMDKSELVQKAKLAEQAE
RYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQ
KTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVF
YLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIR
LGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLI
MQLLRDNLTLWTSENQGDEGDAGEGEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11309
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 54904 Daltons