cDNA Clone slit-robo-GAP 2

• Plasmid: pCMV5-HA slit-robo-GAP 2a
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-slit-robo-GAP 2a
• Genbank: NM_015326
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgatgacgtctccagccaaattcaaaaaggataaggagatcatagcagagtacgatactcaggtcaaagagatccgtgctcagctcacagagcagatgaaatgcctggaccagcagtgtgagcttcgggtgcaactgttgcaggacctccaggacttcttccgaaagaaggcagagattgagatggactactcccgcaacctggagaagctggcagaacgcttcctggccaagacacgcagcaccaaggaccagcaattcaagaaggatcagaatgttctctctccagtcaactgctggaatctcctcttaaaccaggtgaagcgggaaagcagggaccataccaccctgagtgacatctacctgaataatatcattcctcgatttgtacaagtcagcgaggactcaggaagactctttaaaaagagtaaagaagtcggccagcagctccaa
gatgatttgatgaaggtcctgaacgagctctactcggtcatgaagacata
tcacatgtacaatgccgacagcatcagtgctcagagcaaactaaaggagg
cggagaagcaggaggagaagcaaattggtaaatcggtaaagcaggaggac
cggcagaccccacgctcccctgactccacggccaatgttcgcattgagga
gaaacatgtccggaggagctcagtgaagaagattgagaagatgaaggaga
agcgccaagccaagtacacggagaataagctgaaggccatcaaagcccgg
aatgagtacttgctggctttggaggcaaccaatgcatctgtcttcaagta
ctacatccatgacctatctgaccttattgatcagtgttgtgacttaggct
accatgcaagtctgaaccgggctctacgcaccttcctctctgctgagtta
aacctggaacagtcgaagcatgagggtctggatgccatcgagaatgcagt
agaaaacctggatgccaccagtgacaagcagcgcctcatggagatgtaca
acaacgtcttctgcccccctatgaagtttgagtttcagccccacatgggg
gatatggcttcccagctctgtgcccagcagcctgtccagagtgagctggt
acagagatgccaacaactgcagtctcgcttatccactctaaagattgaaa
acgaagaggtaaagaagacaatggaggccaccctgcaaaccatccaggac
attgtgactgtcgaggactttgatgtgtctgactgcttccagtacagcaa
ctccatggagtccgtcaagtccacggtctctgaaaccttcatgagcaagc
ccagcattgctaagaggagagccaaccagcaagagacagagcagttttat
ttcacaaaaatgaaagagtacctggagggcaggaacctcatcaccaagtt
acaagccaagcatgaccttctgcagaaaaccctgggagaaagtcagcgga
cagattgcagtctagccaggcgcagctcaactgtgaggaaacaggactcc
agccaggcaattcctctagtggtggaaagctgtatccggtttatcagcag
acacggactacagcatgaaggaattttccgggtgtcaggatcccaggtgg
aagtgaatgacatcaaaaatgcctttgagagaggagaggaccccctggct
ggggaccagaacgaccatgacatggattccatagctggtgtcctgaagct
ttacttccgggggctggaacaccctctcttccccaaggacatctttcatg
acctgatggcctgcgtcacaatggacaacctgcaggagagagctctgcac
atccggaaagtcctcctagtcctgcccaagaccactctgattatcatgag
atacctctttgccttcctcaatcatttatcacagttcagtgaagagaaca
tgatggacccctacaacctcgccatctgcttcgggccctcgctaatgtca
gtgccagagggccacgaccaggtgtcctgccaagcccacgtgaatgagct
gatcaaaaccatcatcatccagcatgagaacatcttcccaagccccaggg
agctggagggccctgtctacagcagaggaggaagcatggaggattactgt
gatagccctcatggagagactacctcggttgaagactcaacccaggatgt
gaccgcagagcaccacacgagcgatgacgaatgtgagcccatcgaggcca
ttgccaagtttgactacgtgggccggacagcccgagagctgtcctttaag
aagggagcatccctgctgctttaccagcgggcttccgacgactggtggga
aggccggcacaatggcatcgacggactcatcccccatcagtacatcgtgg
tccaagacaccgaggacggtgtcgtggagaggtccagccccaagtctgag
attgaggtcatttctgagccacctgaagaaaaggtgacagccagagcggg
ggccagctgtcccagtgggggtcatgtagccgatatttatcttgcaaaca
tcaacaagcaaaggaagcgtccagaatctgggagcatccggaaaactttt
cggagtgacagccacgggctgagcagttccctgactgactcctcctcccc
aggggtgggggctagctgccgcccatcctcccagcccatcatgagccaga
gcctccccaaagaagggccagataagtgttccatcagtgggcacgggagc
ctcaactccatcagccgccactcatccctgaagaatcggctggatagtcc
acagatccggaagactgccacagcgggaaggtcaaaaagcttcaataacc
atcggcccatggaccctgaggtcattgctcaggatattgaggcaacaatg
aactcggccctgaatgagctacgggaactagaacggcagagcagtgtcaa
acacacccctgacgtggttctggacaccttggagcccctcaaaacctccc
cagtggtggcccccacgtcagagccctccagccctctgcacacccagctc
ctcaaggaccccgagcccgccttccagcgcagcgccagtactgctgggga
catcgcctgcgccttccggcctgtcaagtctgtcaagatggctgccccgg
tcaaaccaccagccacacggcccaagcccactgtcttccccaaaacaaat
gccactagccctggtgtcaactcatcaacttccccacagtctactgacaa
gtcttgtactgtctgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMTSPAKFKKDKEIIAEYDTQVKEIRAQ
LTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAK
TRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPR
FVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISA
QSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKK
IEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLID
QCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQ
RLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRL
STLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVS
ETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKT
LGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFR
VSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLF
PKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLS
QFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHEN
IFPSPRELEGPVYSRGGSMEDYCDSPHGETTSVEDSTQDVTAEHHTSDDE
CEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLI
PHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVA
DIYLANINKQRKRPESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSS
QPIMSQSLPKEGPDKCSISGHGSLNSISRHSSLKNRLDSPQIRKTATAGR
SKSFNNHRPMDPEVIAQDIEATMNSALNELRELERQSSVKHTPDVVLDTL
EPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKS
VKMAAPVKPPATRPKPTVFPKTNATSPGVNSSTSPQSTDKSCTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015326 CDS: g489c, c636t, t705c, g1518a, a1830g, t2565c - all silent.; ; Estimated MW of expressed protein : 123231 Daltons