cDNA Clone TBC1D1

• Plasmid: pGADT7 TBC1D1
• Parent Plasmid: pGADT7
• Expressed: GAL4-AD TBC1D1
• Genbank: NM_015173
• Tag: GAL4 DNA-AD
• Species: Human

Sequence of Insert

ccatggaaccaataacattcacagcaaggaaacatctgctttctaacgaggtctcggtggattttggcctgcagctggtgggctccctgcctgtgcattccctgaccaccatgcccatgctgccctgggttgtggctgaggtgcgaagactcagcaggcagtccaccagaaaggaacctgtaaccaagcaagtccggctttgcgtttcaccctctggactgagatgtgaacctgagccagggagaagtcaacagtgggatcccctgatctattccagcatctttgagtgcaagcctcagcgtgttcacaaactgattcacaacagtcatgacccaagttactttgcttgtctgattaaggaagacgctgtccaccggcagagtatctgctatgtgttcaaagccgatgatcaaacaaaagtgcctgagatcatcagctccatccgtcaggcggggaagatcgcccggcaggaggagctgcactgcccgtccgagttcgacgacacgtttt
ccaagaagttcgaggtgctcttctgcggccgcgtgacggtggcgcacaag
aaggctccgccggccctgatcgacgagtgcatcgagaagttcaatcacgt
cagcggcagccgggggtccgagagcccccgccccaacccgccccatgccg
cgcccacagggagccaggagcctgtgcgcaggcccatgcgcaagtccttc
tcccagcccggcctgcgctcgctggcctttaggaaggagctgcaggatgg
gggcctccgaagcagcggcttcttcagctccttcgaggagagcgacattg
agaaccacctcattagcggacacaatattgtgcagcccacagatatcgag
gaaaatcgaactatgctcttcacgattggccagtctgaagtttacctcat
cagtcctgacaccaaaaaaatagcattggagaaaaattttaaggagatat
ccttttgctctcagggcatcagacacgtggaccactttgggtttatctgt
cgggagtcttccggaggtggcggctttcattttgtctgttacgtgtttca
gtgcacaaatgaggctctggttgatgaaattatgatgaccctgaaacagg
ccttcacggtggccgcagtgcagcagacagctaaggcgccagcccagctg
tgtgagggctgccccctgcaaagcctgcacaagctctgtgagaggataga
gggaatgaattcttccaaaacaaaactagaactgcaaaagcacctgacga
cattaacaaatcaggagcaggcgactatttttgaagaggttcagaaattg
agaccgagaaatgagcagcgagagaatgaattgattatttcttttctgag
atgtttatatgaagagaaacagaaagaacacatccatattggggagatga
agcagacatcgcagatggcagcagagaatattggaagtgaattaccaccc
agtgccactcgatttaggctagatatgctgaaaaacaaagcaaagagatc
tttaacagagtctttagaaagtattttgtcccggggtaataaagccagag
gcctgcaggaacactccatcagtgtggatctggatagctccctgtctagt
acattaagtaacaccagcaaagagccatctgtgtgtgaaaaggaggcctt
gcccatctctgagagctcctttaagctcctcggctcctcggaggacctgt
ccagtgactcggagagtcatctcccagaagagccagctccgctgtcgccc
cagcaggccttcaggaggcgagcaaacaccctgagtcacttccccatcga
atgccaggaacctccacaacctgcccgggggtccccgggggtttcgcaaa
ggaaacttatgaggtatcactcagtgagcacagagacgcctcatgaacga
aaggactttgaatccaaagcaaaccatcttggtgattctggtgggactcc
tgtgaagacccggaggcattcctggaggcagcagatattcctccgagtag
ccaccccgcagaaggcgtgcgattcttccagcagatatgaagattattca
gagctgggagagcttcccccacgatctcctttagaaccagtttgtgaaga
tgggccctttggccccccaccagaggaaaagaaaaggacatctcgtgagc
tccgagagctgtggcaaaaggctattcttcaacagatactgctgcttaga
atggagaaggaaaatcagaagctccaagcctctgaaaatgatttgctgaa
caagcgcctgaagctcgattatgaagaaattactccctgtcttaaagaag
taactacagtgtgggaaaagatgcttagcactccaggaagatcaaaaatt
aagtttgacatggaaaaaatgcactcggctgttgggcaaggtgtgccacg
tcatcaccgaggtgaaatctggaaatttctagctgagcaattccacctta
aacaccagtttcccagcaaacagcagccaaaggatgtgccatacaaagaa
ctcttaaagcagctgacttcccagcagcatgcgattcttattgaccttgg
gcgaacctttcctacacacccatacttctctgcccagcttggagcaggac
agctatcgctttacaacattttgaaggcctactcacttctagaccaggaa
gtgggatattgccaaggtctcagctttgtagcaggcattttgcttcttca
tatgagtgaggaagaggcgtttaaaatgctcaagtttctgatgtttgaca
tggggctgcggaaacagtatcggccagacatgattattttacagatccag
atgtaccagctctcgaggttgcttcatgattaccacagagacctctacaa
tcacctggaggagcacgagatcggccccagcctctacgctgccccctggt
tcctcaccatgtttgcctcacagttcccgctgggattcgtagccagagtc
tttgatatgatttttcttcagggaacagaggtcatatttaaagtggcttt
aagtctgttgggaagccataagcccttgattctgcagcatgaaaacctag
aaaccatagttgactttataaaaagcacgctacccaaccttggcttggta
cagatggaaaagaccatcaatcaggtatttgaaatggacatcgctaaaca
gttacaagcttatgaagttgagtaccacgtccttcaagaagaacttatcg
attcctctcctctcagtgacaaccaaagaatggataaattagagaaaacc
aacagcagcttacgcaaacagaaccttgacctccttgaacagttgcaggt
ggcaaatggtaggatccaaagccttgaggccaccattgagaagctcctga
gcagtgagagcaagctgaagcaggccatgcttaccttagaactggagcgg
tcggccctgctgcagacggtggaggagctgcggcggcggagcgcagagcc
cagcgaccgggagcctgagtgcacgcagcccgagcccacgggcgactgaccatgg

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAME
PITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSR
QSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQRVH
KLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQAGK
IARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNH
VSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQD
GGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEVYL
ISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCYVF
QCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERI
EGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFL
RCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKAKR
SLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEA
LPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPI
ECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSGGT
PVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPVCE
DGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQASENDLL
NKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVP
RHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDL
GRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLL
HMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLY
NHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVA
LSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAK
QLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQ
VANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAE
PSDREPECTQPEPTGD*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11214
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clontech MATCHMAKER vector for yeast Two-Hybrid screen.; Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422)