cDNA Clone PTIP

• Plasmid: pETBlue2.6His hPTIP 590-1069 (end)
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His-PTIP 590-1069 (end)
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCCAGCTTTTTGGACATGATCCAGCAGTGGAGATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGAAGAAAATGGTACCGCCG
CACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATG
TTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGACAGAGATG
ACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTA
TGCCGCAGCAACACAGTCCTCATCTGTAAAGAACCAACTGGTTTAAAGTA
TGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCCAGTGGCTTG
GCGACATTCTTCTGGGAAACTTTGAGGCACTGAGGCAGATTCAGTATAGT
CGCTACACGGCATTCAGTCTGCAGGATCCATTTGCCCCTACCCAGCATTT
AGTTTTAAATCTTTTAGATGCTTGGAGAGTTCCCTTAAAAGTGTCTGCAG
AGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAGAATGAAGTA
GCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACGTACCACC
TCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTG
GATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTACATTCTT
GGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAG
CAAGGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTCGTGAAGC
ACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTC
ATTGATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAGTACTTTT
CTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCT
TTAAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCC
ACTATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCA
GCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGG
AAATAATTTTAATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATAT
TTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGT
GCTCACTCAAACGCTGGACTATGAATCATATAAGTTTAACTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSPEFQLFGHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQL
LATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRERKRCVTAH
WLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLK
LMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDI
LLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELL
MSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFE
PVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTAISVVKHIV
TPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKA
KYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKLMEHKQNSSLSEII
LISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5835
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of N-terminal 6His-tagged protein.