cDNA Clone Pu1 (mouse)

• Plasmid: pCMV5-6His Pu1
• Parent Plasmid: pCMV5
• Genbank: NM_011355
• Tag: 6His

Sequence of Insert

GAATTCGCCACCATGCACCATCACCATCACCATATGTTACAGGCGTGCAAAATGGAAGGGTTTTCCCTCACCGCCCCTCCATCGGATGACTTGGTTACTTACGATTCAGAGCTATACCAACGTCCAATGCATGACTACTACTCCTTCGTGGGCAGCGATGGAGAAAGCCATAGCGATCACTACTGGGATTTCTCCGCACACCATGTCCACAACAACGAGTTTGAGAACTTCCCTGAGAACCACTTCACAGAGCTGCAGAGTGTGCAGCCCCCGCAGCTACAGCAGCTCTATCGCCACATGGAGCTGGAACAGATGCACGTCCTCGATACTCCCATGGTGCCACCCCACACCGGCCTCAGTCACCAGGTTTCCTACATGCCCCGGATGTGCTTCCCTTATCAAACCTTGTCCCCAGCCCACCAGCAGAGCTCAGATGAGGAGGAGGGTGAGAGGCAGAGCCCTCCCCTGGAGGTGTCTGATGGAGAAGCTGATGGCTTGGAGCCTGGGCCAGGTCTTCTGCACGGGGAGACAGGCAGCAAGAAAAAGATTCGCCTGTACCAGTTCCTGCTGGACCTGCTGCGCAGCGGCGACATGAAGGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACCTTCCAGTTCTCGTCCAAGCACAAGGAGGCGCTGGCGCACCGCTGGGGCATCCAGAAGGGCAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGCTGCGCAACTACGGCAAGACAGGCGAGGTGAAGAAAGTCAAGAAGAAGCTCACCTACCAGTTCAGCGGCGAGGTGCTGGGCCGTGGGGGCCTGGCCGAGCGGCGCCTCCCGCCCCACTGAGGATCC

Amino Acid Sequence

MHHHHHHMLQACKMEGFSLTAPPSDDLVTYDSELYQRPMHDYYSFVGSDG
ESHSDHYWDFSAHHVHNNEFENFPENHFTELQSVQPPQLQQLYRHMELEQ
MHVLDTPMVPPHTGLSHQVSYMPRMCFPYQTLSPAHQQSSDEEEGERQSP
PLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWW
VDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEV
KKVKKKLTYQFSGEVLGRGGLAERRLPPH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5742
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Nucleotide sequence difference compared with NM_011355:; c228t (silent S76); ; Estimated MW of expressed protein : 32302