cDNA Clone 53BP1 (mouse)

• Synonyms: TP53BP1
• Plasmid: pHR-SIN-CSGW HA (mouse) 53BP1 (lent)
• Parent Plasmid: pHR SIN CSGW
• Genbank: NM_013735
• Tag: HA
• Species: Mouse

Sequence of Insert

ACTAGTGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGACCCTACTGGAAGTCAATTGGATTCAGATTTCTCTCAGCAAGACACTCCTTGCCTGATAATAGAAGATTCTCAGCCTGAAAGCCAGGTTCTGGAAGAAGATGCAGGCTCTCACTTCAGCGTGCTATCTCGACACCTTCCTAATCTGCAGATGCACAAAGAGAACCCCGTGTTGGATATTGTATCAAATCCGGAACAATCTGCTGTAGAACAAGGAGACAGTAATAGCTCATTCAATGAACATCTGAAAGAAAAGAAAGCTTCAGATCCTGTGGAGTCTTCTCATTTGGGTACCAGTGGTTCCATCAGTCAGGTCATTGAACGGTTACCTCAGCCAAACAGGACAAGCAGTGCTCTGGCAGTGACAGTAGAAGCTGCTTCTCTCCCAGAGGAGGAGAAGGAAGAAGAGGAGTTAGAGGAGAAGGAAGGGGTGGGAGCAAACGCTCCCGGTGCTGACTCCCTTGCTGCTGAAGATTCTGCTTCATCACAGTTGGGCTTTGGAGTTCTGGAACTGTCCCAGAGCCAGGATGTTGAAGAACATACAGTGCCATATGATGTCAACCAAGAGCATCTGCAGTTGGTGACCACTAACTCGGGTTCTAGCCCGCTATCTGATGTGGATGCGAGCACTGCAATTAAATGTGAAGAACAGCCCACTGAAGATATTGCCATGATAGAACAGCCCAGCAAAGACATCCCTGTTACAGTACAGCCCGGTAAAGGTATCCATGTGGTAGAAGAACAAAATCTACCACTTGTAAGGTCCGAAGACCGGCCGTCCAGTCCTCAAGTTTCTGTTGCTGCTGTGGAAACAAAGGAACAGGTACCTGCCCGGGAGCTGCTGGAAGAGGGGCCGCAGGTTCAGCCGTCATCAGAGCCTGAGGTTTCCTCAACCCAGGAGGACTTGTTTGACCAGAGTAGTAAAACAGCTTCTGATGGTTGTTCTACTCCTTCAAGGGAGGAAGGTGGGTGCTCTCCGGTTTCCACACCTGCTACCACCCTGCAGCTCCTGCAGCTCTCTGGTCAGAAGCCCCTTGTTCAGGAGAGTCTTTCCACGAATTCCTCAGATCTTGTTGCTCCTTCCCCTGATGCTTTCCGATCTACCCCTTTTATCGTTCCTAGCAGTCCCACAGAGCAAGGAGGGAGAAAAGATGAGCCCATGGATATGTCAGTGATACCTGCAGGAGGGGAGCCTTTCCAGAAGCTTCATGATGACGAAGCAATGGAGACAGAAAAACCCCTTCTCCCGTCTCAGCCTACTGTGTCACCGCAAGCATCAACACCAGTGTCTCGGAGCACGCCAGTTTTCACTCCTGGCTCTCTTCCCATCCCGTCCCAGCCCGAGTTCTCTCATGACATTTTCATTCCATCACCAAGTCTGGAAGAACCATCAGATGATGTGAAGAAAGGTGGAGGTTTACATAGCTCATCTCTTACTGTTGAGTGTTCCAAGACTTCAGAGAGTGAACCAAAGAATTTCACTGATGACCTTGGGCTCTCCATGACAGGGGATTCTTGCAAACTGATGCTTTCTACAAGTGAGTATAGTCAGTCCTCAAAGATGGAGAGCTTGGGTTCTCCCAGGACTGAGGAAGACAGAGAGAACACACAGATTGACGATACTGAACCTTTGTCTCCAGTTAGCAATTCTAAACTTCCTGCTGACAGTGAGAATGTCCTGGTGACTCCATCGCAGGACGACCAGGTAGAAATGAGTCAGAATGTAGATAAAGCAAAAGAGGATGAAACGGAGGACAGAGGTGACTGTAAAGGCAGAGAAGACGCGGTTGCTGAAGATGTTTGCATCGACCTCACTTGTGATTCTGGGAGTCAGGCAGTTCCTTCTCCAGCTACCCGTTCGGAGGCACTTTCCAGTGTCTTAGATCAGGAGGAAGCTATGGACACTAAAGAACACCATCCAGAGGAAGGGTTTTCGGGATCTGAAGTAGAAGAAGTCCCTGAGACTCCCTGTGGAAGTCACAGAGAGGAGCCCAAAGAAGAAGCGATGGAAAGTATCCCACTGCACCTTTCTCTGACTGAAACCCAGTCTGAGGCATTGTGTCTGCAGAAGGAAGCCCCCAAAGAGGAATGCCCAGAA
GCTATGGAAGTTGAAACCAGTGTGATTAGTATTGACTCCCCCCAGAAACT
GCAAGTACTTGACCAGGAATTAGAGCATAAGGACCCAGACACCTGGGAAG
AAGCTACTTCTGAGGACTCCAGTGTTGTTATCGTTGATGTGAAGGAGCCC
TCTCCTCGAGCTGATGTTTCCTGTGAACCTTTGGAGGAGGTGGAGAAATG
CTCTGACTCCCAGTCCTGGGAAGGTGTGGCTCCAGAGGAAGAACCATGTG
CTGAGAATAGATTAGATACTCCAGAAGAAAAGCGTATAGAATGTGATGGA
GATTCAAAAGCAGAGACCACAGAAAAGGACGCTGTAACAGAAGACTCTCC
ACAACCTCCTTTGCCTTCAGTGAGAGACGAACCTGTCAGACCCGATCAGG
AGACACAGCAGCCCCAAGTTCAAGAGAAAGAGAGCCCAGTGACTGTAGAT
GCAGAAGTGGCTGATGACAAGCAGCTGGGACCAGAGGGCGCATGCCAGCA
GCTTGAGAAGGCCCCTGCCTGTGCCTCACAGAGTTTCTGTGAAAGTTCTA
GTGGGATTAGCAACTATCCAGAAAGCACCATAGCAACCAGTGATGTCACA
TCTGAAAGCATGGTGGAGATCAATGATCCTCTACTTGGGAATGAAAAAGG
GGATTCTGAGTCTGCCCCAGAAATGGATGGAAAACTGTCTCTGAAAATGA
AACTGGTTAGTCCTGAGACAGAGGCCAGTGAAGAATCTTTGCAATTTAGC
CTGGAAAAGCCTACAACTGCTGAGAGAAAAAATGGATCTACTGCCATTGC
AGAGCCTGTTGCCAGTCTCCAGAAGCCGGTGCCTGTGTTTGGCTGCATCT
ATGAAGCCCAGCAAGAGAAGGAGGCTCAAAGTGAGGCTCCTCCCTCTGCA
CCCGACAGGGCGAATTTGCTCCATTTTCCAAGTGCTCAAGAAGAAGACAA
AGAAAGACCAGATGTTACCCCAAAACTCAGGCAGAGTGAACAGCCTGTGA
AGCCTGTTGGGCCTGTCATGGACGATGCTGCTCCCGAGGACTCAGCTTCT
CCTGTTTCGCAGCAGAGGGCCTCACAGGAGCCCTTCAGTCCTGCAGAGGA
CGTGATGGAAACAGACCTGCTGGAAGGACTGGCTGCTAACCAGGACAGAC
CTAGTAAGATGTTGATGGACAGGCCCACCCAGAGTAACATCGGGATCCAG
ACCGTGGACCATTCCCTGTGTGCCCCAGAAACTGTCTCGGCAGCAACGCA
GACTGTGAAGAGTGTGTGTGAGCAGGGGACCAGTACAGCGGAGCAGAACT
CTCGGAAACAAGATGCCACTGTGCAGACTGAGAGGGGGAGTGGAGAGAAA
CCTGCCAGTGCTCCCGTGGACGACACAGAGTCCCTCCACAGCCAGGGGGA
AGAGGAATTTGAAATGCCCCAGCCTCCGCATGGCCATGTCTTGCATCGCC
ACATGAGAACCATTCGAGAAGTCCGTACACTCGTCACCCGCGTCATCACA
GATGTTTATTATGTGGATGGGACAGAAGTGGAAAGGAAAGTAACGGAGGA
GACTGAAGAACCAATCGTAGAGTGTCAGGAATGTGAAACAGAAGTTTCCC
CTTCACAGACTGGAGGTTCCTCTGGGGACCTGGGGGACATCAGCTCCTTC
TCCTCCAAAGCTTCCAGCTCACACCACACATCCAGTGGAACAAGTCTCTC
AGCCATACACAGCAGTGGCAGCTCAGGGCGAGGAGCTGGGCCACTCAAAG
GAAAAGCCAGTGGGACAGAAGCTGCAGATTTTGCCTTACCCAGTTCCCGA
GGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGATCAGTCAGACAGGGGC
ACCGGTGTGTGAGGAAGATGGTGATGCAGGCCTTGGCATCAGACAGGGAG
GGAAGGCTCCAGTTACACCTCGTGGGCGTGGGCGAAGGGGCCGCCCACCT
TCTCGGACCACTGGAACCAGAGAAACAGTTGTCTCTGGTCCGTTGGGCGT
AGAAGATATTTCACCTAGCATGTCACCAGATGACAAGTCCTTCACCCGCA
TTATGCCTCGTGTGCCAGATTCTACCAAACGGACGGATGCCAGTTCTAGT
ACTTTGCGGCGGAGTGATTCTCCAGAGATTCCTTTTCAGGCTGCTACTGG
TTCCTCTGATGGCTTGGATTCCTCATCTTCAGGAAACAGCTTTGTGGGTC
TCCGTGTTGTAGCTAAGTGGTCATCCAATGGCTACTTTTACTCTGGGAAG
ATCACCCGAGATGTGGGGGCTGGGAAGTACAAGCTGCTCTTTGATGATGG
GTACGAATGTGACGTGCTGGGCAAAGACATTCTCCTGTGTGACCCCATCC
CCCTGGACACTGAAGTGACAGCCCTCTCAGAAGATGAATATTTCAGTGCA
GGAGTGGTGAAAGGACATAGGAAGGAGTCTGGGGAGCTGTATTACAGCAT
TGAAAAAGAAGGCCAAAGGAAGTGGTATAAACGAATGGCGGTCATTCTGT
CCTTGGAGCAAGGAAACAGACTGAGAGAGCAATATGGGCTTGGCCCATAT
GAAGCTGTCACACCGCTCACGAAGGCAGCAGATATCAGCTTAGACAATTT
GGTGGAAGGAAAGCGGAAACGGCGCAGTAACATCAGCTCCCCAGTCACCC
CCACTGCCGCCAGTAGCAGCAGCACAACGCCCACACGTAAAGCCACAGAG
AGTCCCCGTGCTTCCACGGGAGTCCCGTCAGGCAAAAGGAAACTTCCGAC
TTCTGAAGAGGAACGCTCTCCTGCTAAGCGAGGTCGAAAGTCTGCCACCG
TGAAACCTGGAACAGTGGGGGCAGCAGAGTTTGTGAGTCCCTGTGAGACT
GGAGACAACATAGGTGAGCCTTCTGTCCTAGAAGAGCCAAGAGGGCCTTT
GCCCCTCAACAAGACCTTGTTTCTGGGCTATGCCTTTCTCCTCACCATGG
CTACAACTAGTGACAAGCTGGCCAGTCGCTCTAAGCTGCTAGATGGTCCT
ACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTAGAAATTCCTCCTTTCAA
CAAGCAGTATACAGAATGCCAGCTTCGAGCAGGAGCTGGGTATATCCTTG
AAGACTTCAATGAAGCCCAGTGTAACACAGCCTACCAGTGTCTCCTAATT
GCGGACCAGCACTGTCGAACCCGGAAGTACTTCCTGTGCCTTGCCAGTGG
CATTCCTTGTGTGTCTCATGTCTGGGTCCATGACAGTTGCCATGCCAACC
AACTTCAAAACTATCGTAATTATCTGCTGCCTGCTGGGTATAGCCTTGAA
GAGCAACGAATTCTGGATTGGCAACCCCGTGAAAACCCTTTCCAGAATCT
GAAGGTCCTCTTGGTGTCAGATCAACAACAGAACTTCTTGGAGCTCTGGT
CTGAGATCCTCATGACTGGAGGGGCAGCCTCTGTGAAGCAGCACCATTCA
AGTGCCCACAACAAAGACATTGCTTTAGGGGTATTTGATGTGGTGGTGAC
AGACCCCTCATGCCCAGCCTCGGTGCTCAAGTGTGCTGAAGCCTTGCAAC
TGCCTGTGGTATCACAAGAATGGGTGATCCAGTGCCTCATTGTTGGGGAG
AGAATTGGATTCAAGCAGCATCCAAAATATAAACATGATTATGTTTCTCA
CTAAACGCGT

Amino Acid Sequence

MYPYDVPDYAMDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEEDAGSHF
SVLSRHLPNLQMHKENPVLDIVSNPEQSAVEQGDSNSSFNEHLKEKKASD
PVESSHLGTSGSISQVIERLPQPNRTSSALAVTVEAASLPEEEKEEEELE
EKEGVGANAPGADSLAAEDSASSQLGFGVLELSQSQDVEEHTVPYDVNQE
HLQLVTTNSGSSPLSDVDASTAIKCEEQPTEDIAMIEQPSKDIPVTVQPG
KGIHVVEEQNLPLVRSEDRPSSPQVSVAAVETKEQVPARELLEEGPQVQP
SSEPEVSSTQEDLFDQSSKTASDGCSTPSREEGGCSPVSTPATTLQLLQL
SGQKPLVQESLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQGGRKDEPMDM
SVIPAGGEPFQKLHDDEAMETEKPLLPSQPTVSPQASTPVSRSTPVFTPG
SLPIPSQPEFSHDIFIPSPSLEEPSDDVKKGGGLHSSSLTVECSKTSESE
PKNFTDDLGLSMTGDSCKLMLSTSEYSQSSKMESLGSPRTEEDRENTQID
DTEPLSPVSNSKLPADSENVLVTPSQDDQVEMSQNVDKAKEDETEDRGDC
KGREDAVAEDVCIDLTCDSGSQAVPSPATRSEALSSVLDQEEAMDTKEHH
PEEGFSGSEVEEVPETPCGSHREEPKEEAMESIPLHLSLTETQSEALCLQ
KEAPKEECPEAMEVETSVISIDSPQKLQVLDQELEHKDPDTWEEATSEDS
SVVIVDVKEPSPRADVSCEPLEEVEKCSDSQSWEGVAPEEEPCAENRLDT
PEEKRIECDGDSKAETTEKDAVTEDSPQPPLPSVRDEPVRPDQETQQPQV
QEKESPVTVDAEVADDKQLGPEGACQQLEKAPACASQSFCESSSGISNYP
ESTIATSDVTSESMVEINDPLLGNEKGDSESAPEMDGKLSLKMKLVSPET
EASEESLQFSLEKPTTAERKNGSTAIAEPVASLQKPVPVFGCIYEAQQEK
EAQSEAPPSAPDRANLLHFPSAQEEDKERPDVTPKLRQSEQPVKPVGPVM
DDAAPEDSASPVSQQRASQEPFSPAEDVMETDLLEGLAANQDRPSKMLMD
RPTQSNIGIQTVDHSLCAPETVSAATQTVKSVCEQGTSTAEQNSRKQDAT
VQTERGSGEKPASAPVDDTESLHSQGEEEFEMPQPPHGHVLHRHMRTIRE
VRTLVTRVITDVYYVDGTEVERKVTEETEEPIVECQECETEVSPSQTGGS
SGDLGDISSFSSKASSSHHTSSGTSLSAIHSSGSSGRGAGPLKGKASGTE
AADFALPSSRGGPGKLSPRKGISQTGAPVCEEDGDAGLGIRQGGKAPVTP
RGRGRRGRPPSRTTGTRETVVSGPLGVEDISPSMSPDDKSFTRIMPRVPD
STKRTDASSSTLRRSDSPEIPFQAATGSSDGLDSSSSGNSFVGLRVVAKW
SSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVT
ALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNR
LREQYGLGPYEAVTPLTKAADISLDNLVEGKRKRRSNISSPVTPTAASSS
STTPTRKATESPRASTGVPSGKRKLPTSEEERSPAKRGRKSATVKPGTVG
AAEFVSPCETGDNIGEPSVLEEPRGPLPLNKTLFLGYAFLLTMATTSDKL
ASRSKLLDGPTGSSEEEEEFLEIPPFNKQYTECQLRAGAGYILEDFNEAQ
CNTAYQCLLIADQHCRTRKYFLCLASGIPCVSHVWVHDSCHANQLQNYRN
YLLPAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILMTG
GAASVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQE
WVIQCLIVGERIGFKQHPKYKHDYVSH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5731
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid for lentiviral expression in mammalian cells; (SFFV promoter); N.B. Nucleotide sequence differences compared with NM_013735 CDS:; Delta nt 2654-2773 (delta aa 885-924). ; t3255c, c3441t, t3468g, a4947g (all silent).