cDNA Clone SLX4 (yeast)

• Plasmid: pGEX6P-3-SLX4
• Parent Plasmid: pGEX6P3
• Genbank: Z73307
• Species: Yeast

Sequence of Insert

GGATCCGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTA
TCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACA
AAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATA
AAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSELQRAQRNLKFLQNEDYVN
VTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSP
MTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLD
DAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKT
KPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNE
GNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQ
KFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQ
SAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGN
VVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIV
SDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGI
IDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKN
LTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEE
ETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIASPEK
FCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNK
GEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSA
EPFVSQIDEMTIREWADVQGICLRNDKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5655
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 111052 Daltons