cDNA Clone RAD51

• Plasmid: pGEX6P-3-RAD51
• Parent Plasmid: pGEX6P3
• Expressed: GST-RAD51
• Genbank: NM_002875
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggcaatgcagatgcagcttgaagcaaatgcagatacttcagtggaagaagaaagctttggcccacaacccatttcacggttagagcagtgtggcataaatgccaacgatgtgaagaaattggaagaagctggattccatactgtggaggctgttgcctatgcgccaaagaaggagctaataaatattaagggaattagtgaagccaaagctgataaaattctggctgaggcagctaaattagttccaatgggtttcaccactgcaactgaattccaccaaaggcggtcagagatcata
cagattactactggctccaaagagcttgacaaactacttcaaggtggaat
tgagactggatctatcacagaaatgtttggagaattccgaactgggaaga
cccagatctgtcatacgctagctgtcacctgccagcttcccattgaccgg
ggtggaggtgaaggaaaggccatgtacattgacactgagggtacctttag
gccagaacggctgctggcagtggctgagaggtatggtctctctggcagtg
atgtcctggataatgtagcatatgctcgagcgttcaacacagaccaccag
acccagctcctttatcaagcatcagccatgatggtagaatctaggtatgc
actgcttattgtagacagtgccaccgccctttacagaacagactactcgg
gtcgaggtgagctttcagccaggcagatgcacttggccaggtttctgcgg
atgcttctgcgactcgctgatgagtttggtgtagcagtggtaatcactaa
tcaggtggtagctcaagtggatggagcagcgatgtttgctgctgatccca
aaaaacctattggaggaaatatcatcgcccatgcatcaacaaccagattg
tatctgaggaaaggaagaggggaaaccagaatctgcaaaatctacgactc
tccctgtcttcctgaagctgaagctatgttcgccattaatgcagatggag
tgggagatgccaaagactgaggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAMQMQLEANADTSVEEES
FGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEA
KADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIET
GSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPE
RLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALL
IVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQV
VAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPC
LPEAEAMFAINADGVGDAKD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5615
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 63787 Daltons