cDNA Clone Pu1 (mouse)

• Plasmid: pEBG6P-1 Pu1
• Parent Plasmid: pEBG6P
• Genbank: NM_011355
• Species: Mouse

Sequence of Insert

GGATCCATGTTACAGGCGTGCAAAATGGAAGGGTTTTCCCTCACCGCCCCTCCATCGGATGACTTGGTTACTTACGATTCAGAGCTATACCAACGTCCAATGCATGACTACTACTCCTTCGTGGGCAGCGATGGAGAAAGCCATAGCGATCACTACTGGGATTTCTCCGCACACCATGTCCACAACAACGAGTTTGAGAACTTCCCTGAGAACCACTTCACAGAGCTGCAGAGTGTGCAGCCCCCGCAGCTACAGCAGCTCTATCGCCACATGGAGCTGGAACAGATGCACGTCCTCGATACTCCC
ATGGTGCCACCCCACACCGGCCTCAGTCACCAGGTTTCCTACATGCCCCG
GATGTGCTTCCCTTATCAAACCTTGTCCCCAGCCCACCAGCAGAGCTCAG
ATGAGGAGGAGGGTGAGAGGCAGAGCCCTCCCCTGGAGGTGTCTGATGGA
GAAGCTGATGGCTTGGAGCCTGGGCCAGGTCTTCTGCACGGGGAGACAGG
CAGCAAGAAAAAGATTCGCCTGTACCAGTTCCTGCTGGACCTGCTGCGCA
GCGGCGACATGAAGGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACC
TTCCAGTTCTCGTCCAAGCACAAGGAGGCGCTGGCGCACCGCTGGGGCAT
CCAGAAGGGCAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGC
TGCGCAACTACGGCAAGACAGGCGAGGTGAAGAAAGTCAAGAAGAAGCTC
ACCTACCAGTTCAGCGGCGAGGTGCTGGGCCGTGGGGGCCTGGCCGAGCG
GCGCCTCCCGCCCCACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLQACKMEGFSLT
APPSDDLVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEF
ENFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVS
YMPRMCFPYQTLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLH
GETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAH
RWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGG
LAERRLPPH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5593
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Pu1 with N-terminal GST (contains preScission protease site); N.B. Nucleotide sequence difference compared with NM_011355:; c228t (silent S76); ; Estimated MW of expressed protein : 59588 Daltons