cDNA Clone Pu1 (mouse)

• Plasmid: pGEX6P-1-Pu1.6His
• Parent Plasmid: pGEX6P1
• Genbank: NM_011355
• Tag: 6His
• Species: Mouse

Sequence of Insert

GGATCCATGTTACAGGCGTGCAAAATGGAAGGGTTTTCCCTCACCGCCCCTCCATCGGATGACTTGGTTACTTACGATTCAGAGCTATACCAACGTCCAATGCATGACTACTACTCCTTCGTGGGCAGCGATGGAGAAAGCCATAGCGATCACTACTGGGATTTCTCCGCACACCATGTCCACAACAACGAGTTTGAGAACTTCCCTGAGAACCACTTCACAGAGCTGCAGAGTGTGCAGCCCCCGCAGCTACAGCAGCTCTATCGCCACATGGAGCTGGAACAGATGCACGTCCTCGATACTCCC
ATGGTGCCACCCCACACCGGCCTCAGTCACCAGGTTTCCTACATGCCCCG
GATGTGCTTCCCTTATCAAACCTTGTCCCCAGCCCACCAGCAGAGCTCAG
ATGAGGAGGAGGGTGAGAGGCAGAGCCCTCCCCTGGAGGTGTCTGATGGA
GAAGCTGATGGCTTGGAGCCTGGGCCAGGTCTTCTGCACGGGGAGACAGG
CAGCAAGAAAAAGATTCGCCTGTACCAGTTCCTGCTGGACCTGCTGCGCA
GCGGCGACATGAAGGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACC
TTCCAGTTCTCGTCCAAGCACAAGGAGGCGCTGGCGCACCGCTGGGGCAT
CCAGAAGGGCAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGC
TGCGCAACTACGGCAAGACAGGCGAGGTGAAGAAAGTCAAGAAGAAGCTC
ACCTACCAGTTCAGCGGCGAGGTGCTGGGCCGTGGGGGCCTGGCCGAGCG
GCGCCTCCCGCCCCACCACCATCACCATCACCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLQACKMEGFSLTAPPSDD
LVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEFENFPEN
HFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVSYMPRMC
FPYQTLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSK
KKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQK
GNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRL
PPHHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5592
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express Pu1 with N-terminal GST and C-terminal 6His tags. ; N.B. Nucleotide sequence difference compared with NM_011355:; c228t (silent S76); ; Estimated MW of expressed protein : 58995 Daltons