cDNA Clone YES1

• Plasmid: pGEX6P-3-YES1
• Parent Plasmid: pGEX6P3
• Expressed: GST-YES1
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAG
ATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAA
TCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCA
GATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAAAAGA
TGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGTATTTTCTTAG
TAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACT
TGACAATGGTGGATACTATATCACAACCAGAGCACAATTTGATACTCTGC
AGAAATTGGTGAAACACTACACAGAACATGCTGATGGTTTATGCCACAAG
TTGACAACTGTGTGTCCAACTGTGAAACCTCAGACTCAAGGTCTAGCAAA
AGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAGGTTAAACTAG
GACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTT
CCTTCAAGAAGCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTC
CACTATATGCTGTTGTTTCTGAAGAACCAATTTACATTGTCACTGAATTT
ATGTCAAAAGGAAGCTTATTAGATTTCCTTAAGGAAGGAGATGGAAAGTA
TTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATTGCTGATGGTA
TGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTT
AGCAAGGTTAATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAAT
TTCCAATCAAATGGACAGCTCCTGAAGCTGCACTGTATGGTCGGTTTACA
ATAAAGTCTGATGTCTGGTCATTTGGAATTCTGCAAACAGAACTAGTAAC
AAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAAGTACTAGAAC
AAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACC
AACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAG
AGCCACAGTACCAGCCAGGAGAAAATTTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMGCIKSKENKSPA
IKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFG
GSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFK
KGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKM
GRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYK
IRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQ
GLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMM
PEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEG
DGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIA
DFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQT
ELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDP
DERPTFEYIQSFLEDYFTATEPQYQPGENL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5579
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.