cDNA Clone SLX1 (yeast)

• Plasmid: pRS416 SLX1PRO.2FLAG
• Parent Plasmid: pRS416
• Genbank: Z36096
• Tag: FLAG
• Species: Yeast

Sequence of Insert

gtcgacGGACCAACGACAAGGACATTACTTTTGCTTAACTCTAAATCTTCGTCAACCTCTCTACCTGCTAAATTAAACTGTCTTTGAAGATTCCTCCAACCAGCCTTTGATTCGCTGTTTCCACTGAAAATTGGTTCATCCCTATCATCCGCGATTTTCTCTCGTTCCATCAATTCTCTCTGCCTTTGTAATTCCCCTTTCTTTTGCTTGTCATTGATTCTTAAATAGTGATTATAAACTGCCACGCTTAAAACCTTCTTACCAATCTCTTGACCAACAATGTACTCATCGAGGAATTTCTTCAAT
GCTTTTGGAGTGGGTATATTCGATAAACGAGACTGTGCCAGTTTCCCTGA
TGCTGTAGCAGCATATCGACCAATTCTACTAGAATAAGCTCTAAAAAAGT
TTTGGCTTGCAGATTTCAACATGAGTGTGAAATGTAATTAGAGTGCCTTG
TCTTCTATGGTTTTGTTTGATGAGCCGTTTGCTTTTCTTTTTAATTTTAA
TATATACCACTGATGACTTATTCCCTTCTATCATCGAAAAAATATTGTCA
AATTAATGTTCTCTTTTTTCAAAAAATATTAAAAAGATAAGAAAGGTACC
ATGTCGCAGAAAATACAGCAGCATCAGTTTCCTGATTTTTATTGTTGTTA
TCTACTACAATCGATTAACAAGAGGCAATCATTTTACGTTGGATCAACTC
CTAACCCAGTACGACGTTTGAGGCAGCATAATGGGAAATTGGCTGTTGGA
GGGGCATATCGAACCAAACGTGACGGTTCAAGGCCCTGGGAAATGATAAT
GATTGTGCGGGGCTTCCCAAGTAAGATAGCAGCTTTACAGTTTGAACATG
CATGGCAACATGGGTACCAAACGCATTATATTGCCGAAAAAGATCGTGTT
GTAAAGCATAAAGCAGGTGGAAGAACTTTACATCACAAAGTTGCATTAAT
GAAGTTACTTTTAAAGCATGAATTTTTTCAAAGGATGAACTTGATAGTCG
AGGTTTTTAATATAAAGGCTTGGGAGGTTTGGAAACAGGACAAATTTTTT
ATTGAAAGAGACAGATTTCCGATTAATATACAAATAAATGAAAATGCGCT
TGAAGAACCAAAGGAGAAAACTGTGGATGTTCTCATGGATCATAGTGATG
AGAATTTAAAAGTGGTGGAAGCTGTATACACTAAAGTCATTGAAAATGAA
AGAAACATATTCGAAACTTTCGAAAAGAAACTAACTACAGGGGTAGTGCG
ATGTGAAATTTGTGAGAAAGAAATTGACTACACGTCTGAAGAGCAAAATC
TGAAGCCGTTTGTAGCGCTTTGCAATAATAAAGACTGTGGGTGTGTTAAC
CACTTGAAATGTCTTCACAGGTACTTTCTAGATGATGAACAGTTGATGGT
AGGGAGAAGAAATTTGATACCTCGAGGTGGGAAATGTCCGAAATGTGATA
TGTTTTGCGACTGGACGACCCTAGTCAAGTTTTCGACAAGAATGAAGTTA
GCTCACGGGAAGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGTAGggatcc

Amino Acid Sequence

MSQKIQQHQFPDFYCCYLLQSINKRQSFYVGSTPNPVRRLRQHNGKLAVG
GAYRTKRDGSRPWEMIMIVRGFPSKIAALQFEHAWQHGYQTHYIAEKDRV
VKHKAGGRTLHHKVALMKLLLKHEFFQRMNLIVEVFNIKAWEVWKQDKFF
IERDRFPINIQINENALEEPKEKTVDVLMDHSDENLKVVEAVYTKVIENE
RNIFETFEKKLTTGVVRCEICEKEIDYTSEEQNLKPFVALCNNKDCGCVN
HLKCLHRYFLDDEQLMVGRRNLIPRGGKCPKCDMFCDWTTLVKFSTRMKL
AHGKDYKDDDDKDYKDDDDK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression construct for expression of SLX1 from its own promoter with C-terminal 2xFLAG tags.