cDNA Clone SLX1

• Plasmid: pEBG6P-1 hSLX1
• Parent Plasmid: pEBG6P
• Expressed: GST-SLX1
• Genbank: NM_024044
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGGGTCCCGCGGGGGTCGCGGCGAGGCCAGGGCGCTTTTTCGGCGTCTACTGCTCTACCTGCCTGAACCCCCGGTACCGGGGCCGCGTCTATGTGGGGTTCACTGTCAACACTGCTCGTCGGGTCCAGCAGCACAATGGGGGCCGCAAAAAAGGCGGGGCCTGGCGGACCAGCGGGCGAGGGCCCTGGGAGATGGTGCTCGTCGTGCACGGCTTCCCGTCCTCCGTGGCCGCCCTTCGGTTTGAGTGGGCTTGGCAGCACCCGCACGCCTCGCGCCGCCTGGCGCACGTGGGGCCTCGC
CTGCGAGGAGAGACAGCCTTCGCTTTCCACCTGCGCGTGCTGGCGCACAT
GCTGCGCGCACCGCCCTGGGCTCGCCTCCCGCTCACGCTGCGCTGGGTGC
GCCCAGACCTCCGCCAGGACCTCTGCCTCCCGCCGCCGCCGCACGTGCCT
CTGGCCTTCGGGCCTCCACCGCCCCAGGCCCCGGCCCCAAGGCGCCGCGC
AGGTCCCTTTGATGACGCGGAGCCTGAGCCAGACCAGGGGGATCCAGGGG
CCTGCTGCTCCCTGTGCGCCCAGACCATCCAGGATGAAGAGGGGCCCTTG
TGTTGCCCCCACCCTGGCTGCCTGCTAAGGGCCCATGTGATCTGCCTGGC
AGAGGAGTTTCTTCAGGAAGAACCAGGGCAGCTTCTGCCCCTAGAGGGCC
AATGCCCTTGCTGTGAGAAGTCACTGCTTTGGGGAGACCTGATCTGGCTG
TGCCAGATGGACACTGAGAAAGAAGTAGAAGACTCAGAATTAGAAGAGGC
ACACTGGACAGACCTGCTGGAGACCTGAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMGPAGVAARPG
RFFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHNGGRKKGGAWRTSGRG
PWEMVLVVHGFPSSVAALRFEWAWQHPHASRRLAHVGPRLRGETAFAFHL
RVLAHMLRAPPWARLPLTLRWVRPDLRQDLCLPPPPHVPLAFGPPPPQAP
APRRRAGPFDDAEPEPDQGDPGACCSLCAQTIQDEEGPLCCPHPGCLLRA
HVICLAEEFLQEEPGQLLPLEGQCPCCEKSLLWGDLIWLCQMDTEKEVED
SELEEAHWTDLLET*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5546
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express hSLX1 with N-terminal GST (contains preScission protease site); N.B. Sequence difference compared with NM_024044 CDS:; c93t; silent in Y31.; ; Estimated MW of expressed protein : 58339 Dalton