cDNA Clone SLX8 (yeast)

• Plasmid: pGEX6P-3-FLAG.SLX8
• Parent Plasmid: pGEX6P3
• Genbank: AAC03214
• Tag: FLAG
• Species: Yeast

Sequence of Insert

ggatccGCCACCATGGACTACAAGGACGACGATGACAAGATGGCAAGAAGACCTGATAACCAAAATCCAGAAGGTGAAAATCTACGAATCAAAAGGGTAAGACTAGAGTCGGTCAGGCAGAACGATGAGGAGGAAGAAAATGAAGTATCGAGAACACAAAATATCGTCACAGATAACCGCCATGACAGCCCAGAGGCCGTAGTCGAAATCATTGGAGAGCGAGCTCTTGAGAATACTTCTGAGGAAGATGGTGATGATGATTTATCTCTCTTTAGAGCACTTGAAGAAGATCCAGGTAGCGACCATAACACCAGTAACAACGATTCTGGGAATCATGACAGGGAAACAATGCATACCGAAGAGCCAGAAGCAAGTTCTGGTAACAATATCACATTAACGAATAATGTTGAAGAATTGCACACAATGGACGTTCTTTCACAAACAGCAAACACTCCGAGTGCTTCCCCGATGCTTGACGCAGCACCGCCGACAACGAAACCTGGAACAAATAGTAAGGAACAAACTGTTGATTTAACTGCAGATGCCATAGACTTAGACGCTGAAGAGCAACAAGTGTTGCAAATATCAGATGACGACTTTCAGGAGGAAACCAAAGAAGCACCCAAAGAGTATGGTGCAGCCAAGGACTATCGCTGCCCGATTTGTTTTGAACCTCCAGAGACAGCCCTCATGACGTTATGTGGGCACGTCTTTTGTTGTCCATGCTTATTTCAAATGGTTAATAGCTCAAGAACATGTAGACAGTTCGGCCATTGCGCTCTTTGTAGAAGTAAAGTGTACTTAAAGGATGTGAGGTTAATTATTCTAAGGAAAAAGCAAGTCAAGAAGAAGGTAAAAAGCTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMDYKDDDDKMARRPDNQ
NPEGENLRIKRVRLESVRQNDEEEENEVSRTQNIVTDNRHDSPEAVVEII
GERALENTSEEDGDDDLSLFRALEEDPGSDHNTSNNDSGNHDRETMHTEE
PEASSGNNITLTNNVEELHTMDVLSQTANTPSASPMLDAAPPTTKPGTNS
KEQTVDLTADAIDLDAEEQQVLQISDDDFQEETKEAPKEYGAAKDYRCPI
CFEPPETALMTLCGHVFCCPCLFQMVNSSRTCRQFGHCALCRSKVYLKDV
RLIILRKKQVKKKVKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5409
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion of FLAG.SLX8.; ; Estimated MW of expressed protein : 58848 Daltons