cDNA Clone SIK

• Plasmid: pFastBac.HTb SIK 1-E281
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-SIK 1-E281
• Genbank: NM_173354
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAAAAATAATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGCTTCTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTAC
ATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTC
CAACGGGCACCTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGCAAATCC
TGTCGGCCGTGGAGTACTGTCACGACCATCACATCGTCCACCGGGACCTC
AAGACCGAGAACCTCCTGCTGGATGGCAACATGGACATCAAGCTGGCAGA
TTTTGGATTTGGGAATTTCTACAAGTCAGGAGAGCCTCTGTCCACGTGGT
GTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAGGAGTAT
GAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTACGTCCT
GGTCTGCGGTTCTCTCCCCTTCGATGGGCCTAACCTGCCGACGCTGAGAC
AGCGGGTGCTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCTCAAGAC
TGTGAGAGCCTGATCCGCCGCATGCTGGTGGTGGACCCCGCCAGGCGCAT
CACCATCGCCCAGATCCGGCAGCACCGGTGGATGCGGGCTGAGTGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMVIMSEFSADPAGQGQGQQKPL
RVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIY
REVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLS
ENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDIKLADFGFGN
FYKSGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSL
PFDGPNLPTLRQRVLEGRFRIPFFMSQDCESLIRRMLVVDPARRITIAQI
RQHRWMRAE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5385
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 35770 Daltons