cDNA Clone SLX1 (yeast)

• Plasmid: pETDuet-1 6his.SLX4/SLX1
• Parent Plasmid: pETDuet1
• Genbank: SLX4:Z73307 SLX1:Z36097
• Tag: 6His
• Species: Yeast

Sequence of Insert

DNA sequence of insert 1 (SLX4) :
ggatccgGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACA
AAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATA
AAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGggatcc

DNA sequence of insert 2 (SLX1) :
agatctcTCGCAGAAAATACAGCAGCATCAGTTTCCTGATTTTTATTGTTGTTATCTACTACAATCGATTAACAAGAGGCAATCATTTTACGTTGGATCAACTCCTAACCCAGTACGACGTTTGAGGCAGCATAATGGGAAATTGGCTGTTGGAGGGGCATATCGAACCAAACGTGACGGTTCAAGGCCCTGGGAAATGATAAT
GATTGTGCGGGGCTTCCCAAGTAAGATAGCAGCTTTACAGTTTGAACATG
CATGGCAACATGGGTACCAAACGCATTATATTGCCGAAAAAGATCGTGTT
GTAAAGCATAAAGCAGGTGGAAGAACTTTACATCACAAAGTTGCATTAAT
GAAGTTACTTTTAAAGCATGAATTTTTTCAAAGGATGAACTTGATAGTCG
AGGTTTTTAATATAAAGGCTTGGGAGGTTTGGAAACAGGACAAATTTTTT
ATTGAAAGAGACAGATTTCCGATTAATATACAAATAAATGAAAATGCGCT
TGAAGAACCAAAGGAGAAAACTGTGGATGTTCTCATGGATCATAGTGATG
AGAATTTAAAAGTGGTGGAAGCTGTATACACTAAAGTCATTGAAAATGAA
AGAAACATATTCGAAACTTTCGAAAAGAAACTAACTACAGGGGTAGTGCG
ATGTGAAATTTGTGAGAAAGAAATTGACTACACGTCTGAAGAGCAAAATC
TGAAGCCGTTTGTAGCGCTTTGCAATAATAAAGACTGTGGGTGTGTTAAC
CACTTGAAATGTCTTCACAGGTACTTTCTAGATGATGAACAGTTGATGGT
AGGGAGAAGAAATTTGATACCTCGAGGTGGGAAATGTCCGAAATGTGATA
TGTTTTGCGACTGGACGACCCTAGTCAAGTTTTCGACAAGAATGAAGTTA
GCTCACGGGAAGTAGagatct

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (SLX4) : MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLER
IYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*

Amino acid sequence of expressed protein 2 (SLX1) : MADLSQKIQQHQFPDFYCCYLLQSINKRQSFYVGSTPNPVRRLRQHNGKLAVGGAYRTKRDGSRPWEMIMIVRGFPSKIAALQFEHAWQHGYQTHYIAEKDRVVKHKAGGRTLHHKVALMKLLLKHEFFQRMNLIVEVFNIKAWEVWKQDKFFIERDRFPINIQINENALEEPKEKTVDVLMDHSDENLKVVEAVYTKVIENER
NIFETFEKKLTTGVVRCEICEKEIDYTSEEQNLKPFVALCNNKDCGCVNH
LKCLHRYFLDDEQLMVGRRNLIPRGGKCPKCDMFCDWTTLVKFSTRMKLA
HGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector for dual expression of SLX4 with N-terminal 6His tag and SLX1 without tag.