cDNA Clone PLEKHE1

• Plasmid: pGEX6P-1-PLEKHE1
• Parent Plasmid: pGEX6P1
• Expressed: GST-PLEKHE1
• Genbank: NM_194449
• Tag: GST
• Species: Human

Sequence of Insert

gaattcGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGGAAAACCTCACAGCACGGGTAGCTCTGAACGGATTCAGCTCTCAGGAATGTATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAGACGCCAAGTC
ATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGACAGCTTGAC
CGGAAAGATGCATGTTCTGCCACTAATTGGTGGAAAAGTAGAAGAAGTGA
AAAAGCACCAACACTGTTTAGCATTTAGCTCCTCTGGACCCCAAAGCCAG
ACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAGGTGGCTGCG
ACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGGACCTCTCGT
GTTGTAGCCTGGAACATCTGCCTGCCAACCTCTTCTACAGCCAAGACCTC
ACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACCCTAGCCTTCC
AGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGTTGAAGAGTC
TTAACCTTTCCAATAATCATTTAGGGGACTTCCCTCTGGCAGTCTGCAGT
ATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGCCCTGCGATCAGT
CCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTTTGTTGGATG
GAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATGAAGCAGCTT
AGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACATTCCCGAAGTATT
GGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGAAACTGTGTGG
AGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATTAAACATGTG
GATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGATGAAGTGGACTT
TCTACAGCATGTTACTCAGCTTGACCTACGAGACAATAAGCTTGGTGATC
TAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGTGAAAGGAAT
CAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGCGCTCTATGC
CTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAGTTCCAAATTATC
TGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGTGCCTGAGTGG
GTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCATAATCAAAT
ATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTCTCCGGAAACTAC
TGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGGCTAGAAAGAACC
TCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGAGCTCCCACC
TAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACGCCTCTGCGA
ACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAAGAGACAAACAGT
ATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAGACAAATGTGT
GCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACATGGCCTATA
ACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAACTGGAGGAACTT
GAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCATCCCAACAACGAT
CATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCAACTGCATCG
AGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGTGTGGACCTG
AGCTGTAATGAGCTAAGTGAAGTCACATTACCAGAAAACCTGCCTCCCAA
ACTGCAGGAGCTAGACCTGACTGGAAACCCGCGCCTTGTCCTTGATCACA
AAACCCTGGAACTACTGAATAATATCCGCTGTTTCAAGATTGATCAGCCT
TCTACAGGAGACGCTTCCGGAGCCCCAGCTGTATGGAGTCATGGTTACAC
TGAAGCTTCGGGGGTAAAAAACAAGTTGTGTGTCGCAGCCCTGTCGGTGA
ATAACTTCTGTGACAACCGCGAAGCCCTGTATGGTGTGTTTGACGGAGAC
CGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACTATGAGTGACATTTT
GGCTGAAGAGCTGCAAAAAACAAAAAACGAAGAAGAATACATGGTCAATA
CATTCATTGTCATGCAAAGGAAACTTGGAACTGCTGGGCAGAAGCTTGGT
GGTGCCGCTGTCCTTTGTCATATCAAGCATGACCCTGTGGATCCAGGAGG
ATCCTTCACCTTGACCTCTGCTAATGTGGGCAAGTGCCAAACAGTTCTCT
GTCGAAATGGAAAGCCGCTGCCTCTGTCCAGATCTTACATCATGAGCTGT
GAAGAAGAGCTGAAGAGGATTAAACAGCACAAGGCCATTATCACTGAGGA
TGGCAAGGTGAACGGAGTGACTGAGTCCACGCGCATCCTGGGCTACACCT
TCCTCCATCCCAGTGTGGTGCCTCGCCCCCACGTGCAGTCCGTGCTCCTG
ACTCCCCAGGATGAGTTCTTCATCCTAGGCAGTAAGGGGTTGTGGGACAG
CCTGTCCGTCGAGGAGGCCGTGGAAGCCGTGCGCAACGTGCCCGATGCCC
TGGCTGCTGCCAAGAAGCTGTGTACCCTGGCCCAGAGCTACGGCTGCCAC
GACAGCATCAGCGCTGTGGTGGTGCAGCTCAGTGTCACTGAGGACAGCTT
CTGCTGCTGCGAGCTCAGCGCCGGTGGGGCTGTGCCACCACCCAGTCCTG
GCATCTTTCCTCCCTCAGTGAACATGGTGATCAAGGATCGGCCCTCAGAT
GGGCTGGGCGTGCCGTCCTCCAGCAGCGGCATGGCTTCCGAGATTAGCAG
TGAGCTCTCCACTTCTGAGATGAGCAGCGAGGTGGGGTCAACAGCCTCCG
ATGAGCCCCCGCCCGGAGCCCTAAGCGAGAACAGCCCTGCCTACCCCAGT
GAGCAGCGCTGCATGCTCCACCCCATCTGTCTGTCCAACTCCTTCCAGCG
CCAGCTATCCAGCGCCACGTTCTCTAGCGCCTTCTCCGACAACGGCCTTG
ACAGTGACGATGAGGAGCCCATCGAGGGCGTCTTCACCAACGGCAGCCGG
GTGGAGGTGGAGGTGGACATCCACTGCAGCCGGGCCAAGGAGAAGGAGAA
ACAGCAGCACCTGCTTCAGGTGCCAGCAGAGGCCAGTGATGAGGGCATTG
TCATCAGCGCCAACGAGGATGAGCCAGGTCTGCCCAGGAAGGCAGACTTC
TCTGCCGTTGGGACCATTGGGCGCCGGAGGGCCAATGGCTCTGTTGCGCC
CCAGGAAAGGAGCCACAATGTGATAGAGGTGGCTACAGACGCACCTCTTC
GAAAGCCTGGAGGCTATTTTGCTGCCCCGGCTCAGCCGGATCCTGATGAT
CAGTTTATCATACCCCCGGAGCTGGAAGAGGAGGTCAAAGAAATCATGAA
GCATCACCAGGAGCAACAGCAGCAGCAGCAGCCGCCACCACCCCCTCAGC
TCCAGCCGCAGCTGCCGCGGCACTACCAGCTGGACCAGCTGCCAGATTAT
TACGACACGCCACTATGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDSEIGCLIRFYAGKPH
STGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLT
GKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLR
QVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLP
AARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSV
PAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVL
EKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDF
LQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYA
SSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQI
CELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPP
NLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCV
PLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTI
MNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPK
LQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYT
EASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDIL
AEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGG
SFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITED
GKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDS
LSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSF
CCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISS
ELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQR
QLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEK
QQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAP
QERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMK
HHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence difference compared with NM_194449 CDS:; a585t (silent in P195), present in other PLEKHE1 mRNA sequences.; Estimated MW of expressed protein : 160528 Daltons