cDNA Clone SIK

• Plasmid: pGEX6P-1-HA-SIK 293-353
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA-SIK 293-353
• Genbank: XM_041314
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAAGAGCA
AGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTTCTGCGACTGA
TGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAAC
AAGAGCTATAACCACTTTGCTGCCATTTATTTCTTGTTGGTGGAGCGCCT
GAAATCACATCGGAGCAGTTTCCCATAGggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1357
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express QIK UBA domain with N-terminal GST and HA tags